Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS16620 and LU632_RS16625 are separated by 70 nucleotides LU632_RS16625 and LU632_RS16630 are separated by 25 nucleotides LU632_RS16630 and LU632_RS16635 are separated by 37 nucleotides LU632_RS16635 and LU632_RS16640 are separated by 62 nucleotides LU632_RS16640 and LU632_RS16645 are separated by 103 nucleotides
LU632_RS16620: LU632_RS16620 - site-specific integrase, at 3,141,738 to 3,142,835
_RS16620
LU632_RS16625: LU632_RS16625 - hypothetical protein, at 3,142,906 to 3,143,220
_RS16625
LU632_RS16630: LU632_RS16630 - type II toxin-antitoxin system HicB family antitoxin, at 3,143,246 to 3,143,656
_RS16630
LU632_RS16635: LU632_RS16635 - glycosyltransferase family 9 protein, at 3,143,694 to 3,144,620
_RS16635
LU632_RS16640: LU632_RS16640 - IS1 family transposase, at 3,144,683 to 3,145,380
_RS16640
LU632_RS16645: LU632_RS16645 - hypothetical protein, at 3,145,484 to 3,145,846
_RS16645
Position (kb)
3143
3144
3145 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3142.742 kb on - strand at 3142.764 kb on - strand at 3142.771 kb on + strand at 3142.771 kb on + strand at 3142.771 kb on + strand at 3142.772 kb on - strand at 3142.772 kb on - strand at 3143.229 kb on - strand at 3143.232 kb on + strand at 3143.306 kb on - strand, within LU632_RS16630 at 3143.308 kb on + strand, within LU632_RS16630 at 3143.308 kb on + strand, within LU632_RS16630 at 3143.380 kb on + strand, within LU632_RS16630 at 3143.380 kb on + strand, within LU632_RS16630 at 3143.380 kb on + strand, within LU632_RS16630 at 3143.380 kb on + strand, within LU632_RS16630 at 3143.381 kb on - strand, within LU632_RS16630 at 3143.557 kb on + strand, within LU632_RS16630 at 3143.630 kb on - strand at 3143.635 kb on + strand at 3143.688 kb on + strand at 3143.689 kb on - strand at 3143.721 kb on + strand at 3143.721 kb on + strand at 3143.722 kb on - strand at 3143.722 kb on - strand at 3143.733 kb on - strand at 3143.783 kb on + strand at 3143.783 kb on + strand at 3143.811 kb on + strand, within LU632_RS16635 at 3143.813 kb on + strand, within LU632_RS16635 at 3143.813 kb on + strand, within LU632_RS16635 at 3143.813 kb on + strand, within LU632_RS16635 at 3143.813 kb on + strand, within LU632_RS16635 at 3143.819 kb on + strand, within LU632_RS16635 at 3143.880 kb on + strand, within LU632_RS16635 at 3143.907 kb on + strand, within LU632_RS16635 at 3143.907 kb on + strand, within LU632_RS16635 at 3143.908 kb on - strand, within LU632_RS16635 at 3143.908 kb on - strand, within LU632_RS16635 at 3143.913 kb on + strand, within LU632_RS16635 at 3143.925 kb on - strand, within LU632_RS16635 at 3143.958 kb on - strand, within LU632_RS16635 at 3143.958 kb on - strand, within LU632_RS16635 at 3144.011 kb on + strand, within LU632_RS16635 at 3144.112 kb on - strand, within LU632_RS16635 at 3144.192 kb on + strand, within LU632_RS16635 at 3144.314 kb on + strand, within LU632_RS16635 at 3144.374 kb on - strand, within LU632_RS16635 at 3144.528 kb on + strand at 3144.529 kb on - strand at 3144.572 kb on + strand at 3145.405 kb on + strand at 3145.483 kb on + strand at 3145.526 kb on + strand, within LU632_RS16645 at 3145.527 kb on - strand, within LU632_RS16645 at 3145.617 kb on + strand, within LU632_RS16645
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 3,142,742 - -0.4 3,142,764 - -0.9 3,142,771 + -0.5 3,142,771 + -0.3 3,142,771 + -3.0 3,142,772 - -0.8 3,142,772 - +0.7 3,143,229 - -1.3 3,143,232 + +0.3 3,143,306 - LU632_RS16630 0.15 +1.3 3,143,308 + LU632_RS16630 0.15 +1.5 3,143,308 + LU632_RS16630 0.15 +1.6 3,143,380 + LU632_RS16630 0.33 -0.2 3,143,380 + LU632_RS16630 0.33 -0.7 3,143,380 + LU632_RS16630 0.33 +0.3 3,143,380 + LU632_RS16630 0.33 -0.3 3,143,381 - LU632_RS16630 0.33 -0.5 3,143,557 + LU632_RS16630 0.76 +0.0 3,143,630 - +0.3 3,143,635 + -0.1 3,143,688 + +1.0 3,143,689 - +0.7 3,143,721 + -0.8 3,143,721 + +2.0 3,143,722 - +0.3 3,143,722 - -0.4 3,143,733 - -0.2 3,143,783 + +0.0 3,143,783 + +0.2 3,143,811 + LU632_RS16635 0.13 -0.5 3,143,813 + LU632_RS16635 0.13 +0.6 3,143,813 + LU632_RS16635 0.13 +0.6 3,143,813 + LU632_RS16635 0.13 -0.4 3,143,813 + LU632_RS16635 0.13 +1.5 3,143,819 + LU632_RS16635 0.13 +0.5 3,143,880 + LU632_RS16635 0.20 -0.0 3,143,907 + LU632_RS16635 0.23 -0.7 3,143,907 + LU632_RS16635 0.23 -0.3 3,143,908 - LU632_RS16635 0.23 -0.5 3,143,908 - LU632_RS16635 0.23 -1.1 3,143,913 + LU632_RS16635 0.24 -0.7 3,143,925 - LU632_RS16635 0.25 +0.5 3,143,958 - LU632_RS16635 0.28 -1.3 3,143,958 - LU632_RS16635 0.28 -0.4 3,144,011 + LU632_RS16635 0.34 -0.7 3,144,112 - LU632_RS16635 0.45 -0.1 3,144,192 + LU632_RS16635 0.54 -0.2 3,144,314 + LU632_RS16635 0.67 +0.4 3,144,374 - LU632_RS16635 0.73 -0.6 3,144,528 + -0.2 3,144,529 - -0.4 3,144,572 + -1.2 3,145,405 + +0.6 3,145,483 + -0.5 3,145,526 + LU632_RS16645 0.12 -0.9 3,145,527 - LU632_RS16645 0.12 -0.1 3,145,617 + LU632_RS16645 0.37 +0.7
Or see this region's nucleotide sequence