Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpoB and LU632_RS14770 are separated by 41 nucleotides LU632_RS14770 and hinT are separated by 49 nucleotides hinT and ptsG are separated by 190 nucleotides ptsG and LU632_RS14785 are separated by 257 nucleotides
LU632_RS14765: lpoB - penicillin-binding protein activator LpoB, at 2,807,352 to 2,807,918
lpoB
LU632_RS14770: LU632_RS14770 - YcfL family protein, at 2,807,960 to 2,808,307
_RS14770
LU632_RS14775: hinT - purine nucleoside phosphoramidase, at 2,808,357 to 2,808,707
hinT
LU632_RS14780: ptsG - PTS glucose transporter subunit IIBC, at 2,808,898 to 2,810,331
ptsG
LU632_RS14785: LU632_RS14785 - metal-dependent hydrolase, at 2,810,589 to 2,811,383
_RS14785
Position (kb)
2808
2809
2810
2811 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2807.934 kb on - strand at 2807.934 kb on - strand at 2807.961 kb on + strand at 2807.967 kb on + strand at 2807.986 kb on + strand at 2807.986 kb on + strand at 2807.987 kb on - strand at 2807.987 kb on - strand at 2808.096 kb on + strand, within LU632_RS14770 at 2808.097 kb on - strand, within LU632_RS14770 at 2808.112 kb on - strand, within LU632_RS14770 at 2808.116 kb on - strand, within LU632_RS14770 at 2808.208 kb on + strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.263 kb on - strand, within LU632_RS14770 at 2808.320 kb on + strand at 2808.351 kb on + strand at 2808.449 kb on + strand, within hinT at 2808.573 kb on - strand, within hinT at 2808.653 kb on + strand, within hinT at 2808.654 kb on - strand, within hinT at 2808.678 kb on - strand at 2808.683 kb on + strand at 2808.831 kb on + strand at 2808.831 kb on + strand at 2808.832 kb on - strand at 2808.900 kb on - strand at 2808.914 kb on + strand at 2808.914 kb on + strand at 2808.914 kb on + strand at 2808.915 kb on - strand at 2808.924 kb on + strand at 2809.098 kb on + strand, within ptsG at 2809.189 kb on - strand, within ptsG at 2809.207 kb on + strand, within ptsG at 2809.208 kb on - strand, within ptsG at 2809.226 kb on + strand, within ptsG at 2809.292 kb on - strand, within ptsG at 2809.292 kb on - strand, within ptsG at 2809.354 kb on - strand, within ptsG at 2809.419 kb on + strand, within ptsG at 2809.420 kb on - strand, within ptsG at 2809.529 kb on - strand, within ptsG at 2809.529 kb on - strand, within ptsG at 2809.530 kb on + strand, within ptsG at 2809.530 kb on + strand, within ptsG at 2809.530 kb on + strand, within ptsG at 2809.531 kb on - strand, within ptsG at 2809.531 kb on - strand, within ptsG at 2809.556 kb on + strand, within ptsG at 2809.588 kb on - strand, within ptsG at 2809.613 kb on + strand, within ptsG at 2809.613 kb on + strand, within ptsG at 2809.656 kb on + strand, within ptsG at 2809.695 kb on + strand, within ptsG at 2809.740 kb on - strand, within ptsG at 2809.742 kb on - strand, within ptsG at 2809.742 kb on - strand, within ptsG at 2809.866 kb on + strand, within ptsG at 2809.925 kb on + strand, within ptsG at 2809.925 kb on + strand, within ptsG at 2809.976 kb on - strand, within ptsG at 2809.979 kb on - strand, within ptsG at 2810.067 kb on + strand, within ptsG at 2810.332 kb on - strand at 2810.337 kb on + strand at 2810.413 kb on - strand at 2810.522 kb on + strand at 2810.531 kb on + strand at 2810.532 kb on - strand at 2810.557 kb on - strand at 2810.563 kb on - strand at 2810.572 kb on + strand at 2810.572 kb on + strand at 2810.572 kb on + strand at 2810.693 kb on + strand, within LU632_RS14785 at 2810.693 kb on + strand, within LU632_RS14785 at 2810.693 kb on + strand, within LU632_RS14785 at 2810.694 kb on - strand, within LU632_RS14785 at 2810.695 kb on + strand, within LU632_RS14785 at 2810.695 kb on + strand, within LU632_RS14785 at 2810.696 kb on - strand, within LU632_RS14785 at 2810.696 kb on - strand, within LU632_RS14785 at 2810.696 kb on - strand, within LU632_RS14785 at 2810.716 kb on + strand, within LU632_RS14785 at 2810.717 kb on - strand, within LU632_RS14785 at 2810.762 kb on - strand, within LU632_RS14785 at 2810.782 kb on - strand, within LU632_RS14785 at 2810.803 kb on + strand, within LU632_RS14785 at 2810.803 kb on + strand, within LU632_RS14785 at 2810.803 kb on + strand, within LU632_RS14785 at 2810.803 kb on + strand, within LU632_RS14785 at 2810.804 kb on - strand, within LU632_RS14785 at 2810.864 kb on + strand, within LU632_RS14785 at 2810.866 kb on + strand, within LU632_RS14785 at 2810.973 kb on - strand, within LU632_RS14785 at 2811.035 kb on + strand, within LU632_RS14785 at 2811.036 kb on - strand, within LU632_RS14785 at 2811.088 kb on + strand, within LU632_RS14785 at 2811.177 kb on + strand, within LU632_RS14785 at 2811.178 kb on - strand, within LU632_RS14785 at 2811.199 kb on + strand, within LU632_RS14785 at 2811.199 kb on + strand, within LU632_RS14785 at 2811.200 kb on - strand, within LU632_RS14785 at 2811.200 kb on - strand, within LU632_RS14785 at 2811.244 kb on + strand, within LU632_RS14785 at 2811.276 kb on - strand, within LU632_RS14785 at 2811.309 kb on + strand at 2811.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,807,934 - +0.8 2,807,934 - +0.3 2,807,961 + +0.2 2,807,967 + +1.0 2,807,986 + +0.7 2,807,986 + -1.1 2,807,987 - -1.5 2,807,987 - -0.6 2,808,096 + LU632_RS14770 0.39 +1.7 2,808,097 - LU632_RS14770 0.39 +1.0 2,808,112 - LU632_RS14770 0.44 +0.5 2,808,116 - LU632_RS14770 0.45 +0.1 2,808,208 + LU632_RS14770 0.71 -0.5 2,808,209 - LU632_RS14770 0.72 +0.0 2,808,209 - LU632_RS14770 0.72 -0.2 2,808,209 - LU632_RS14770 0.72 +0.0 2,808,209 - LU632_RS14770 0.72 -0.8 2,808,263 - LU632_RS14770 0.87 -0.5 2,808,320 + -1.3 2,808,351 + +2.0 2,808,449 + hinT LU632_RS14775 0.26 -0.8 2,808,573 - hinT LU632_RS14775 0.62 -0.2 2,808,653 + hinT LU632_RS14775 0.84 -0.6 2,808,654 - hinT LU632_RS14775 0.85 -1.0 2,808,678 - -0.4 2,808,683 + -1.0 2,808,831 + +0.6 2,808,831 + +0.5 2,808,832 - -1.5 2,808,900 - +0.1 2,808,914 + +0.5 2,808,914 + +1.0 2,808,914 + +0.0 2,808,915 - -0.9 2,808,924 + +0.3 2,809,098 + ptsG LU632_RS14780 0.14 +0.6 2,809,189 - ptsG LU632_RS14780 0.20 -0.4 2,809,207 + ptsG LU632_RS14780 0.22 +0.0 2,809,208 - ptsG LU632_RS14780 0.22 -0.3 2,809,226 + ptsG LU632_RS14780 0.23 -0.0 2,809,292 - ptsG LU632_RS14780 0.27 +1.0 2,809,292 - ptsG LU632_RS14780 0.27 -0.5 2,809,354 - ptsG LU632_RS14780 0.32 -0.2 2,809,419 + ptsG LU632_RS14780 0.36 +0.5 2,809,420 - ptsG LU632_RS14780 0.36 -0.1 2,809,529 - ptsG LU632_RS14780 0.44 +1.8 2,809,529 - ptsG LU632_RS14780 0.44 +0.5 2,809,530 + ptsG LU632_RS14780 0.44 +0.0 2,809,530 + ptsG LU632_RS14780 0.44 -0.4 2,809,530 + ptsG LU632_RS14780 0.44 +0.1 2,809,531 - ptsG LU632_RS14780 0.44 +0.1 2,809,531 - ptsG LU632_RS14780 0.44 -0.9 2,809,556 + ptsG LU632_RS14780 0.46 -0.5 2,809,588 - ptsG LU632_RS14780 0.48 -0.4 2,809,613 + ptsG LU632_RS14780 0.50 -0.1 2,809,613 + ptsG LU632_RS14780 0.50 -0.3 2,809,656 + ptsG LU632_RS14780 0.53 -0.3 2,809,695 + ptsG LU632_RS14780 0.56 -0.5 2,809,740 - ptsG LU632_RS14780 0.59 +0.5 2,809,742 - ptsG LU632_RS14780 0.59 +0.6 2,809,742 - ptsG LU632_RS14780 0.59 +0.1 2,809,866 + ptsG LU632_RS14780 0.68 -0.5 2,809,925 + ptsG LU632_RS14780 0.72 -0.3 2,809,925 + ptsG LU632_RS14780 0.72 +0.7 2,809,976 - ptsG LU632_RS14780 0.75 +0.5 2,809,979 - ptsG LU632_RS14780 0.75 -2.1 2,810,067 + ptsG LU632_RS14780 0.82 -0.0 2,810,332 - -0.5 2,810,337 + +0.5 2,810,413 - +0.6 2,810,522 + -0.6 2,810,531 + +0.4 2,810,532 - +0.0 2,810,557 - +0.1 2,810,563 - -0.5 2,810,572 + -0.7 2,810,572 + +0.0 2,810,572 + -0.3 2,810,693 + LU632_RS14785 0.13 -0.7 2,810,693 + LU632_RS14785 0.13 +0.2 2,810,693 + LU632_RS14785 0.13 +0.0 2,810,694 - LU632_RS14785 0.13 -0.4 2,810,695 + LU632_RS14785 0.13 -0.8 2,810,695 + LU632_RS14785 0.13 +0.4 2,810,696 - LU632_RS14785 0.13 +0.8 2,810,696 - LU632_RS14785 0.13 -0.1 2,810,696 - LU632_RS14785 0.13 -1.2 2,810,716 + LU632_RS14785 0.16 -0.2 2,810,717 - LU632_RS14785 0.16 -0.1 2,810,762 - LU632_RS14785 0.22 -0.2 2,810,782 - LU632_RS14785 0.24 +0.3 2,810,803 + LU632_RS14785 0.27 -0.0 2,810,803 + LU632_RS14785 0.27 -0.0 2,810,803 + LU632_RS14785 0.27 +0.7 2,810,803 + LU632_RS14785 0.27 +2.0 2,810,804 - LU632_RS14785 0.27 -1.5 2,810,864 + LU632_RS14785 0.35 +0.5 2,810,866 + LU632_RS14785 0.35 +0.0 2,810,973 - LU632_RS14785 0.48 -0.9 2,811,035 + LU632_RS14785 0.56 -0.1 2,811,036 - LU632_RS14785 0.56 -0.6 2,811,088 + LU632_RS14785 0.63 -0.7 2,811,177 + LU632_RS14785 0.74 -0.7 2,811,178 - LU632_RS14785 0.74 +0.4 2,811,199 + LU632_RS14785 0.77 +0.6 2,811,199 + LU632_RS14785 0.77 -0.3 2,811,200 - LU632_RS14785 0.77 -0.1 2,811,200 - LU632_RS14785 0.77 -0.9 2,811,244 + LU632_RS14785 0.82 -0.5 2,811,276 - LU632_RS14785 0.86 +0.3 2,811,309 + +0.3 2,811,310 - +0.6
Or see this region's nucleotide sequence