Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS14225 and LU632_RS14230 overlap by 4 nucleotides LU632_RS14230 and LU632_RS14235 are separated by 60 nucleotides LU632_RS14235 and LU632_RS14240 overlap by 4 nucleotides
LU632_RS14225: LU632_RS14225 - replication protein P, at 2,726,867 to 2,727,628
_RS14225
LU632_RS14230: LU632_RS14230 - hypothetical protein, at 2,727,625 to 2,727,867
_RS14230
LU632_RS14235: LU632_RS14235 - helix-turn-helix domain-containing protein, at 2,727,928 to 2,728,304
_RS14235
LU632_RS14240: LU632_RS14240 - phage antirepressor KilAC domain-containing protein, at 2,728,301 to 2,729,002
_RS14240
Position (kb)
2727
2728 Strain fitness (log2 ratio)
-1
0
1 at 2726.859 kb on - strand at 2726.907 kb on + strand at 2726.910 kb on - strand at 2726.910 kb on - strand at 2726.910 kb on - strand at 2726.910 kb on - strand at 2726.910 kb on - strand at 2726.910 kb on - strand at 2726.910 kb on - strand at 2727.169 kb on - strand, within LU632_RS14225 at 2728.243 kb on - strand, within LU632_RS14235 at 2728.584 kb on + strand, within LU632_RS14240 at 2728.584 kb on + strand, within LU632_RS14240 at 2728.835 kb on + strand, within LU632_RS14240 at 2728.835 kb on + strand, within LU632_RS14240 at 2728.848 kb on + strand, within LU632_RS14240
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,726,859 - -1.0 2,726,907 + -0.2 2,726,910 - -1.4 2,726,910 - -0.7 2,726,910 - -0.0 2,726,910 - -0.5 2,726,910 - -0.8 2,726,910 - +0.9 2,726,910 - +0.2 2,727,169 - LU632_RS14225 0.40 -0.6 2,728,243 - LU632_RS14235 0.84 -0.2 2,728,584 + LU632_RS14240 0.40 +0.5 2,728,584 + LU632_RS14240 0.40 +0.1 2,728,835 + LU632_RS14240 0.76 +0.6 2,728,835 + LU632_RS14240 0.76 +0.8 2,728,848 + LU632_RS14240 0.78 +0.7
Or see this region's nucleotide sequence