Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cmoB and LU632_RS13465 are separated by 554 nucleotides LU632_RS13465 and LU632_RS13470 overlap by 12 nucleotides LU632_RS13470 and LU632_RS13475 overlap by 8 nucleotides LU632_RS13475 and cutC are separated by 574 nucleotides
LU632_RS13460: cmoB - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 2,567,622 to 2,568,590
cmoB
LU632_RS13465: LU632_RS13465 - cytochrome B, at 2,569,145 to 2,569,252
_RS13465
LU632_RS13470: LU632_RS13470 - hypothetical protein, at 2,569,241 to 2,570,014
_RS13470
LU632_RS13475: LU632_RS13475 - hypothetical protein, at 2,570,007 to 2,570,417
_RS13475
LU632_RS13480: cutC - copper homeostasis protein CutC, at 2,570,992 to 2,571,747
cutC
Position (kb)
2569
2570
2571 Strain fitness (log2 ratio)
-1
0
1 at 2568.248 kb on - strand, within cmoB at 2568.345 kb on + strand, within cmoB at 2568.359 kb on + strand, within cmoB at 2568.360 kb on - strand, within cmoB at 2568.392 kb on - strand, within cmoB at 2568.657 kb on + strand at 2568.666 kb on + strand at 2568.680 kb on + strand at 2568.680 kb on + strand at 2568.681 kb on - strand at 2568.681 kb on - strand at 2568.681 kb on - strand at 2568.723 kb on - strand at 2568.729 kb on + strand at 2568.729 kb on + strand at 2568.730 kb on - strand at 2568.887 kb on + strand at 2568.902 kb on - strand at 2568.949 kb on + strand at 2568.953 kb on - strand at 2568.953 kb on - strand at 2568.964 kb on + strand at 2568.965 kb on - strand at 2568.980 kb on - strand at 2569.080 kb on + strand at 2569.147 kb on - strand at 2569.196 kb on + strand, within LU632_RS13465 at 2569.211 kb on + strand, within LU632_RS13465 at 2569.211 kb on + strand, within LU632_RS13465 at 2569.211 kb on + strand, within LU632_RS13465 at 2569.283 kb on - strand at 2569.296 kb on - strand at 2569.802 kb on + strand, within LU632_RS13470 at 2569.803 kb on - strand, within LU632_RS13470 at 2569.804 kb on + strand, within LU632_RS13470 at 2569.805 kb on - strand, within LU632_RS13470 at 2569.805 kb on - strand, within LU632_RS13470 at 2569.847 kb on + strand, within LU632_RS13470 at 2569.847 kb on + strand, within LU632_RS13470 at 2569.885 kb on - strand, within LU632_RS13470 at 2570.060 kb on - strand, within LU632_RS13475 at 2570.060 kb on - strand, within LU632_RS13475 at 2570.328 kb on - strand, within LU632_RS13475 at 2570.419 kb on + strand at 2570.446 kb on + strand at 2570.450 kb on + strand at 2570.451 kb on - strand at 2570.453 kb on - strand at 2570.476 kb on - strand at 2570.565 kb on - strand at 2570.650 kb on + strand at 2570.653 kb on + strand at 2570.654 kb on - strand at 2570.679 kb on + strand at 2570.734 kb on + strand at 2570.734 kb on + strand at 2570.735 kb on - strand at 2570.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,568,248 - cmoB LU632_RS13460 0.65 -0.4 2,568,345 + cmoB LU632_RS13460 0.75 -1.6 2,568,359 + cmoB LU632_RS13460 0.76 -0.3 2,568,360 - cmoB LU632_RS13460 0.76 +0.2 2,568,392 - cmoB LU632_RS13460 0.79 +0.0 2,568,657 + +1.3 2,568,666 + +0.6 2,568,680 + +0.0 2,568,680 + +0.1 2,568,681 - +0.9 2,568,681 - +0.1 2,568,681 - -0.4 2,568,723 - +1.0 2,568,729 + -0.3 2,568,729 + -0.5 2,568,730 - +0.6 2,568,887 + -0.6 2,568,902 - +0.0 2,568,949 + +0.2 2,568,953 - -0.8 2,568,953 - +0.5 2,568,964 + +0.2 2,568,965 - +0.0 2,568,980 - +0.4 2,569,080 + +1.4 2,569,147 - -0.4 2,569,196 + LU632_RS13465 0.47 -0.6 2,569,211 + LU632_RS13465 0.61 -0.8 2,569,211 + LU632_RS13465 0.61 -0.5 2,569,211 + LU632_RS13465 0.61 +0.0 2,569,283 - -0.7 2,569,296 - +0.6 2,569,802 + LU632_RS13470 0.72 +0.3 2,569,803 - LU632_RS13470 0.73 +0.2 2,569,804 + LU632_RS13470 0.73 +0.3 2,569,805 - LU632_RS13470 0.73 +1.0 2,569,805 - LU632_RS13470 0.73 +0.3 2,569,847 + LU632_RS13470 0.78 -0.2 2,569,847 + LU632_RS13470 0.78 +0.2 2,569,885 - LU632_RS13470 0.83 +1.0 2,570,060 - LU632_RS13475 0.13 +0.0 2,570,060 - LU632_RS13475 0.13 -0.2 2,570,328 - LU632_RS13475 0.78 -0.4 2,570,419 + +0.2 2,570,446 + +0.0 2,570,450 + +0.1 2,570,451 - -0.9 2,570,453 - -0.0 2,570,476 - +0.1 2,570,565 - +0.0 2,570,650 + -0.1 2,570,653 + -0.2 2,570,654 - -0.5 2,570,679 + -0.2 2,570,734 + -0.6 2,570,734 + +0.3 2,570,735 - +0.1 2,570,863 - +0.0
Or see this region's nucleotide sequence