Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS13160 and LU632_RS13165 are separated by 122 nucleotides LU632_RS13165 and LU632_RS13170 are separated by 37 nucleotides LU632_RS13170 and pabB are separated by 111 nucleotides pabB and LU632_RS13180 overlap by 8 nucleotides LU632_RS13180 and sdaA are separated by 365 nucleotides
LU632_RS13160: LU632_RS13160 - hypothetical protein, at 2,512,262 to 2,512,906
_RS13160
LU632_RS13165: LU632_RS13165 - hypothetical protein, at 2,513,029 to 2,513,232
_RS13165
LU632_RS13170: LU632_RS13170 - YoaH family protein, at 2,513,270 to 2,513,455
_RS13170
LU632_RS13175: pabB - aminodeoxychorismate synthase component 1, at 2,513,567 to 2,514,931
pabB
LU632_RS13180: LU632_RS13180 - CoA pyrophosphatase, at 2,514,924 to 2,515,497
_RS13180
LU632_RS13185: sdaA - L-serine ammonia-lyase, at 2,515,863 to 2,517,227
sdaA
Position (kb)
2513
2514
2515 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2512.674 kb on + strand, within LU632_RS13160 at 2512.674 kb on + strand, within LU632_RS13160 at 2512.674 kb on + strand, within LU632_RS13160 at 2512.674 kb on + strand, within LU632_RS13160 at 2512.675 kb on - strand, within LU632_RS13160 at 2512.675 kb on - strand, within LU632_RS13160 at 2512.703 kb on + strand, within LU632_RS13160 at 2512.716 kb on + strand, within LU632_RS13160 at 2512.716 kb on + strand, within LU632_RS13160 at 2512.716 kb on + strand, within LU632_RS13160 at 2512.717 kb on - strand, within LU632_RS13160 at 2512.717 kb on - strand, within LU632_RS13160 at 2512.811 kb on + strand, within LU632_RS13160 at 2512.812 kb on - strand, within LU632_RS13160 at 2512.839 kb on - strand, within LU632_RS13160 at 2512.922 kb on - strand at 2513.100 kb on + strand, within LU632_RS13165 at 2513.139 kb on + strand, within LU632_RS13165 at 2513.219 kb on - strand at 2513.262 kb on + strand at 2513.321 kb on - strand, within LU632_RS13170 at 2513.450 kb on - strand at 2513.450 kb on - strand at 2513.483 kb on + strand at 2513.483 kb on + strand at 2513.484 kb on - strand at 2513.543 kb on + strand at 2513.544 kb on - strand at 2513.558 kb on + strand at 2513.558 kb on + strand at 2513.567 kb on - strand at 2513.579 kb on + strand at 2513.579 kb on + strand at 2513.580 kb on - strand at 2513.594 kb on + strand at 2513.594 kb on + strand at 2513.596 kb on + strand at 2513.701 kb on + strand at 2513.702 kb on - strand at 2513.702 kb on - strand at 2513.702 kb on - strand at 2513.971 kb on - strand, within pabB at 2513.972 kb on + strand, within pabB at 2513.972 kb on + strand, within pabB at 2513.972 kb on + strand, within pabB at 2513.972 kb on + strand, within pabB at 2513.972 kb on + strand, within pabB at 2513.973 kb on - strand, within pabB at 2514.050 kb on - strand, within pabB at 2514.137 kb on + strand, within pabB at 2514.137 kb on + strand, within pabB at 2514.138 kb on - strand, within pabB at 2514.604 kb on + strand, within pabB at 2514.746 kb on + strand, within pabB at 2514.779 kb on - strand, within pabB at 2514.781 kb on + strand, within pabB at 2514.781 kb on + strand, within pabB at 2514.782 kb on - strand, within pabB at 2514.785 kb on - strand, within pabB at 2514.957 kb on - strand at 2515.040 kb on + strand, within LU632_RS13180 at 2515.212 kb on + strand, within LU632_RS13180 at 2515.212 kb on + strand, within LU632_RS13180 at 2515.212 kb on + strand, within LU632_RS13180 at 2515.589 kb on + strand at 2515.592 kb on + strand at 2515.592 kb on + strand at 2515.593 kb on - strand at 2515.593 kb on - strand at 2515.611 kb on + strand at 2515.612 kb on - strand at 2515.618 kb on + strand at 2515.619 kb on - strand at 2515.624 kb on - strand at 2515.636 kb on - strand at 2515.671 kb on + strand at 2515.679 kb on - strand at 2515.679 kb on - strand at 2515.734 kb on - strand at 2515.811 kb on - strand at 2515.811 kb on - strand at 2515.813 kb on + strand at 2515.814 kb on - strand at 2515.816 kb on + strand at 2515.840 kb on - strand at 2515.869 kb on - strand at 2515.916 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,512,674 + LU632_RS13160 0.64 +0.7 2,512,674 + LU632_RS13160 0.64 +0.2 2,512,674 + LU632_RS13160 0.64 -0.5 2,512,674 + LU632_RS13160 0.64 -0.8 2,512,675 - LU632_RS13160 0.64 +0.3 2,512,675 - LU632_RS13160 0.64 +0.7 2,512,703 + LU632_RS13160 0.68 +1.3 2,512,716 + LU632_RS13160 0.70 -0.3 2,512,716 + LU632_RS13160 0.70 -0.4 2,512,716 + LU632_RS13160 0.70 +0.4 2,512,717 - LU632_RS13160 0.71 +0.4 2,512,717 - LU632_RS13160 0.71 +0.1 2,512,811 + LU632_RS13160 0.85 -1.0 2,512,812 - LU632_RS13160 0.85 +0.7 2,512,839 - LU632_RS13160 0.89 -0.6 2,512,922 - -0.6 2,513,100 + LU632_RS13165 0.35 -0.8 2,513,139 + LU632_RS13165 0.54 +0.0 2,513,219 - +0.2 2,513,262 + -1.0 2,513,321 - LU632_RS13170 0.27 -1.0 2,513,450 - -0.7 2,513,450 - -0.6 2,513,483 + -1.0 2,513,483 + +0.4 2,513,484 - -1.9 2,513,543 + +0.0 2,513,544 - -0.3 2,513,558 + +0.7 2,513,558 + -0.8 2,513,567 - -0.3 2,513,579 + -0.8 2,513,579 + +0.3 2,513,580 - +0.1 2,513,594 + -0.7 2,513,594 + +1.3 2,513,596 + +0.5 2,513,701 + -0.3 2,513,702 - +0.4 2,513,702 - -0.0 2,513,702 - +0.4 2,513,971 - pabB LU632_RS13175 0.30 +0.6 2,513,972 + pabB LU632_RS13175 0.30 +1.0 2,513,972 + pabB LU632_RS13175 0.30 -1.0 2,513,972 + pabB LU632_RS13175 0.30 +0.4 2,513,972 + pabB LU632_RS13175 0.30 -0.7 2,513,972 + pabB LU632_RS13175 0.30 +0.3 2,513,973 - pabB LU632_RS13175 0.30 -0.7 2,514,050 - pabB LU632_RS13175 0.35 +0.5 2,514,137 + pabB LU632_RS13175 0.42 -0.0 2,514,137 + pabB LU632_RS13175 0.42 +1.0 2,514,138 - pabB LU632_RS13175 0.42 +0.1 2,514,604 + pabB LU632_RS13175 0.76 -0.3 2,514,746 + pabB LU632_RS13175 0.86 +0.2 2,514,779 - pabB LU632_RS13175 0.89 +0.4 2,514,781 + pabB LU632_RS13175 0.89 +0.3 2,514,781 + pabB LU632_RS13175 0.89 -0.3 2,514,782 - pabB LU632_RS13175 0.89 -1.4 2,514,785 - pabB LU632_RS13175 0.89 -1.3 2,514,957 - -0.4 2,515,040 + LU632_RS13180 0.20 -0.1 2,515,212 + LU632_RS13180 0.50 -1.6 2,515,212 + LU632_RS13180 0.50 -0.8 2,515,212 + LU632_RS13180 0.50 -0.4 2,515,589 + +0.1 2,515,592 + -0.2 2,515,592 + -0.7 2,515,593 - -0.3 2,515,593 - +0.6 2,515,611 + -0.8 2,515,612 - +0.9 2,515,618 + -0.1 2,515,619 - +0.7 2,515,624 - +0.4 2,515,636 - +0.2 2,515,671 + +0.7 2,515,679 - +2.4 2,515,679 - -0.1 2,515,734 - +0.3 2,515,811 - -0.9 2,515,811 - -0.8 2,515,813 + +1.0 2,515,814 - +0.8 2,515,816 + -0.8 2,515,840 - +0.1 2,515,869 - -0.0 2,515,916 + -1.3
Or see this region's nucleotide sequence