Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purU and LU632_RS12945 are separated by 171 nucleotides LU632_RS12945 and xthA are separated by 270 nucleotides xthA and LU632_RS12955 are separated by 34 nucleotides LU632_RS12955 and LU632_RS12960 overlap by 1 nucleotides
LU632_RS12940: purU - formyltetrahydrofolate deformylase, at 2,467,481 to 2,468,329
purU
LU632_RS12945: LU632_RS12945 - tRNA-Tyr, at 2,468,501 to 2,468,585
_RS12945
LU632_RS12950: xthA - exodeoxyribonuclease III, at 2,468,856 to 2,469,662
xthA
LU632_RS12955: LU632_RS12955 - YnjH family protein, at 2,469,697 to 2,470,038
_RS12955
LU632_RS12960: LU632_RS12960 - DNA topoisomerase III, at 2,470,038 to 2,471,948
_RS12960
Position (kb)
2468
2469
2470 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2467.896 kb on + strand, within purU at 2467.939 kb on + strand, within purU at 2467.973 kb on + strand, within purU at 2467.974 kb on - strand, within purU at 2467.991 kb on + strand, within purU at 2467.991 kb on + strand, within purU at 2467.991 kb on + strand, within purU at 2467.991 kb on + strand, within purU at 2467.992 kb on - strand, within purU at 2467.994 kb on - strand, within purU at 2467.994 kb on - strand, within purU at 2468.020 kb on - strand, within purU at 2468.090 kb on + strand, within purU at 2468.102 kb on + strand, within purU at 2468.103 kb on - strand, within purU at 2468.103 kb on - strand, within purU at 2468.141 kb on + strand, within purU at 2468.176 kb on + strand, within purU at 2468.203 kb on + strand, within purU at 2468.204 kb on - strand, within purU at 2468.204 kb on - strand, within purU at 2468.207 kb on + strand, within purU at 2468.207 kb on + strand, within purU at 2468.208 kb on - strand, within purU at 2468.221 kb on + strand, within purU at 2468.221 kb on + strand, within purU at 2468.222 kb on - strand, within purU at 2468.303 kb on + strand at 2468.327 kb on + strand at 2468.414 kb on - strand at 2468.651 kb on + strand at 2468.651 kb on + strand at 2468.651 kb on + strand at 2468.724 kb on + strand at 2468.725 kb on - strand at 2468.749 kb on + strand at 2468.749 kb on + strand at 2468.749 kb on + strand at 2468.752 kb on - strand at 2468.769 kb on + strand at 2468.769 kb on + strand at 2468.975 kb on + strand, within xthA at 2468.975 kb on + strand, within xthA at 2468.975 kb on + strand, within xthA at 2468.975 kb on + strand, within xthA at 2468.975 kb on + strand, within xthA at 2468.975 kb on + strand, within xthA at 2468.976 kb on - strand, within xthA at 2468.976 kb on - strand, within xthA at 2468.976 kb on - strand, within xthA at 2468.976 kb on - strand, within xthA at 2469.021 kb on + strand, within xthA at 2469.022 kb on - strand, within xthA at 2469.042 kb on + strand, within xthA at 2469.181 kb on - strand, within xthA at 2469.259 kb on - strand, within xthA at 2469.292 kb on + strand, within xthA at 2469.293 kb on - strand, within xthA at 2469.296 kb on + strand, within xthA at 2469.296 kb on + strand, within xthA at 2469.314 kb on + strand, within xthA at 2469.315 kb on - strand, within xthA at 2469.315 kb on - strand, within xthA at 2469.446 kb on + strand, within xthA at 2469.446 kb on + strand, within xthA at 2469.447 kb on - strand, within xthA at 2469.506 kb on + strand, within xthA at 2469.506 kb on + strand, within xthA at 2469.597 kb on + strand at 2469.598 kb on - strand at 2469.682 kb on - strand at 2469.699 kb on - strand at 2469.738 kb on - strand, within LU632_RS12955 at 2469.763 kb on + strand, within LU632_RS12955 at 2469.769 kb on + strand, within LU632_RS12955 at 2469.769 kb on + strand, within LU632_RS12955 at 2469.770 kb on - strand, within LU632_RS12955 at 2469.801 kb on + strand, within LU632_RS12955 at 2469.802 kb on - strand, within LU632_RS12955 at 2469.804 kb on - strand, within LU632_RS12955 at 2469.819 kb on - strand, within LU632_RS12955 at 2469.888 kb on + strand, within LU632_RS12955 at 2469.889 kb on - strand, within LU632_RS12955 at 2469.889 kb on - strand, within LU632_RS12955 at 2469.889 kb on - strand, within LU632_RS12955 at 2469.889 kb on - strand, within LU632_RS12955 at 2469.892 kb on + strand, within LU632_RS12955 at 2469.892 kb on + strand, within LU632_RS12955 at 2469.892 kb on + strand, within LU632_RS12955 at 2469.892 kb on + strand, within LU632_RS12955 at 2469.892 kb on + strand, within LU632_RS12955 at 2469.893 kb on - strand, within LU632_RS12955 at 2469.893 kb on - strand, within LU632_RS12955 at 2469.917 kb on + strand, within LU632_RS12955 at 2469.939 kb on + strand, within LU632_RS12955 at 2469.966 kb on - strand, within LU632_RS12955 at 2470.024 kb on - strand at 2470.029 kb on + strand at 2470.039 kb on + strand at 2470.040 kb on - strand at 2470.064 kb on + strand at 2470.065 kb on - strand at 2470.065 kb on - strand at 2470.175 kb on + strand at 2470.187 kb on - strand at 2470.190 kb on + strand at 2470.190 kb on + strand at 2470.307 kb on + strand, within LU632_RS12960 at 2470.307 kb on + strand, within LU632_RS12960 at 2470.308 kb on - strand, within LU632_RS12960 at 2470.310 kb on - strand, within LU632_RS12960 at 2470.322 kb on + strand, within LU632_RS12960 at 2470.443 kb on - strand, within LU632_RS12960 at 2470.458 kb on + strand, within LU632_RS12960 at 2470.459 kb on - strand, within LU632_RS12960 at 2470.461 kb on + strand, within LU632_RS12960
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,467,896 + purU LU632_RS12940 0.49 -0.6 2,467,939 + purU LU632_RS12940 0.54 -0.1 2,467,973 + purU LU632_RS12940 0.58 +1.8 2,467,974 - purU LU632_RS12940 0.58 -0.3 2,467,991 + purU LU632_RS12940 0.60 +0.3 2,467,991 + purU LU632_RS12940 0.60 -0.7 2,467,991 + purU LU632_RS12940 0.60 -1.0 2,467,991 + purU LU632_RS12940 0.60 +0.8 2,467,992 - purU LU632_RS12940 0.60 -0.6 2,467,994 - purU LU632_RS12940 0.60 -0.0 2,467,994 - purU LU632_RS12940 0.60 -0.2 2,468,020 - purU LU632_RS12940 0.63 -0.2 2,468,090 + purU LU632_RS12940 0.72 +0.3 2,468,102 + purU LU632_RS12940 0.73 +0.3 2,468,103 - purU LU632_RS12940 0.73 -0.2 2,468,103 - purU LU632_RS12940 0.73 +0.1 2,468,141 + purU LU632_RS12940 0.78 -0.1 2,468,176 + purU LU632_RS12940 0.82 -0.1 2,468,203 + purU LU632_RS12940 0.85 +0.8 2,468,204 - purU LU632_RS12940 0.85 +0.8 2,468,204 - purU LU632_RS12940 0.85 +1.0 2,468,207 + purU LU632_RS12940 0.86 +0.1 2,468,207 + purU LU632_RS12940 0.86 -0.1 2,468,208 - purU LU632_RS12940 0.86 +0.7 2,468,221 + purU LU632_RS12940 0.87 +0.2 2,468,221 + purU LU632_RS12940 0.87 +0.3 2,468,222 - purU LU632_RS12940 0.87 +0.7 2,468,303 + -0.6 2,468,327 + +0.1 2,468,414 - -0.1 2,468,651 + -1.0 2,468,651 + -0.8 2,468,651 + -0.3 2,468,724 + -1.6 2,468,725 - +0.1 2,468,749 + +0.6 2,468,749 + +0.2 2,468,749 + +0.3 2,468,752 - +0.4 2,468,769 + +0.0 2,468,769 + +1.0 2,468,975 + xthA LU632_RS12950 0.15 +0.9 2,468,975 + xthA LU632_RS12950 0.15 +0.2 2,468,975 + xthA LU632_RS12950 0.15 +1.0 2,468,975 + xthA LU632_RS12950 0.15 -0.2 2,468,975 + xthA LU632_RS12950 0.15 +0.6 2,468,975 + xthA LU632_RS12950 0.15 -1.3 2,468,976 - xthA LU632_RS12950 0.15 -0.3 2,468,976 - xthA LU632_RS12950 0.15 -1.0 2,468,976 - xthA LU632_RS12950 0.15 -0.1 2,468,976 - xthA LU632_RS12950 0.15 -0.8 2,469,021 + xthA LU632_RS12950 0.20 +0.7 2,469,022 - xthA LU632_RS12950 0.21 -2.0 2,469,042 + xthA LU632_RS12950 0.23 -0.5 2,469,181 - xthA LU632_RS12950 0.40 -0.4 2,469,259 - xthA LU632_RS12950 0.50 -0.8 2,469,292 + xthA LU632_RS12950 0.54 -0.1 2,469,293 - xthA LU632_RS12950 0.54 -0.2 2,469,296 + xthA LU632_RS12950 0.55 -0.7 2,469,296 + xthA LU632_RS12950 0.55 -0.2 2,469,314 + xthA LU632_RS12950 0.57 +0.2 2,469,315 - xthA LU632_RS12950 0.57 -0.5 2,469,315 - xthA LU632_RS12950 0.57 -0.7 2,469,446 + xthA LU632_RS12950 0.73 -1.3 2,469,446 + xthA LU632_RS12950 0.73 -0.7 2,469,447 - xthA LU632_RS12950 0.73 -0.6 2,469,506 + xthA LU632_RS12950 0.81 -1.6 2,469,506 + xthA LU632_RS12950 0.81 +0.8 2,469,597 + -1.6 2,469,598 - -0.5 2,469,682 - +1.0 2,469,699 - +1.0 2,469,738 - LU632_RS12955 0.12 -0.1 2,469,763 + LU632_RS12955 0.19 -0.1 2,469,769 + LU632_RS12955 0.21 -1.0 2,469,769 + LU632_RS12955 0.21 -1.3 2,469,770 - LU632_RS12955 0.21 +0.1 2,469,801 + LU632_RS12955 0.30 -1.3 2,469,802 - LU632_RS12955 0.31 -0.8 2,469,804 - LU632_RS12955 0.31 +0.4 2,469,819 - LU632_RS12955 0.36 +0.4 2,469,888 + LU632_RS12955 0.56 +0.0 2,469,889 - LU632_RS12955 0.56 -0.1 2,469,889 - LU632_RS12955 0.56 +0.1 2,469,889 - LU632_RS12955 0.56 +0.7 2,469,889 - LU632_RS12955 0.56 -0.3 2,469,892 + LU632_RS12955 0.57 +0.3 2,469,892 + LU632_RS12955 0.57 -0.3 2,469,892 + LU632_RS12955 0.57 -0.8 2,469,892 + LU632_RS12955 0.57 -0.3 2,469,892 + LU632_RS12955 0.57 -2.0 2,469,893 - LU632_RS12955 0.57 +0.7 2,469,893 - LU632_RS12955 0.57 +0.5 2,469,917 + LU632_RS12955 0.64 +0.0 2,469,939 + LU632_RS12955 0.71 +2.6 2,469,966 - LU632_RS12955 0.79 -0.3 2,470,024 - +0.0 2,470,029 + -0.3 2,470,039 + -0.2 2,470,040 - -0.2 2,470,064 + -0.2 2,470,065 - -0.1 2,470,065 - +1.0 2,470,175 + -0.7 2,470,187 - -1.0 2,470,190 + -1.0 2,470,190 + -0.2 2,470,307 + LU632_RS12960 0.14 -0.6 2,470,307 + LU632_RS12960 0.14 +1.0 2,470,308 - LU632_RS12960 0.14 +1.0 2,470,310 - LU632_RS12960 0.14 -0.5 2,470,322 + LU632_RS12960 0.15 -0.2 2,470,443 - LU632_RS12960 0.21 +0.0 2,470,458 + LU632_RS12960 0.22 -0.2 2,470,459 - LU632_RS12960 0.22 +1.0 2,470,461 + LU632_RS12960 0.22 +0.4
Or see this region's nucleotide sequence