Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnpA and LU632_RS12665 are separated by 97 nucleotides LU632_RS12665 and LU632_RS12670 are separated by 10 nucleotides LU632_RS12670 and LU632_RS12675 overlap by 14 nucleotides LU632_RS12675 and LU632_RS12680 are separated by 248 nucleotides LU632_RS12680 and LU632_RS12685 overlap by 80 nucleotides LU632_RS12685 and LU632_RS12690 are separated by 27 nucleotides
LU632_RS12660: tnpA - IS200/IS605 family transposase, at 2,409,943 to 2,410,308
tnpA
LU632_RS12665: LU632_RS12665 - YdbL family protein, at 2,410,406 to 2,410,732
_RS12665
LU632_RS12670: LU632_RS12670 - YnbE family lipoprotein, at 2,410,743 to 2,410,931
_RS12670
LU632_RS12675: LU632_RS12675 - YdbH family protein, at 2,410,918 to 2,413,545
_RS12675
LU632_RS12680: LU632_RS12680 - 2-hydroxyacid dehydrogenase, at 2,413,794 to 2,414,051
_RS12680
LU632_RS12685: LU632_RS12685 - hypothetical protein, at 2,413,972 to 2,414,172
_RS12685
LU632_RS12690: LU632_RS12690 - N-acetylmuramoyl-L-alanine amidase, at 2,414,200 to 2,414,742
_RS12690
Position (kb)
2410
2411
2412
2413
2414 Strain fitness (log2 ratio)
-2
-1
0
1 at 2410.345 kb on + strand at 2410.345 kb on + strand at 2410.449 kb on + strand, within LU632_RS12665 at 2410.449 kb on + strand, within LU632_RS12665 at 2410.449 kb on + strand, within LU632_RS12665 at 2410.450 kb on + strand, within LU632_RS12665 at 2410.669 kb on + strand, within LU632_RS12665 at 2410.669 kb on + strand, within LU632_RS12665 at 2410.720 kb on - strand at 2410.788 kb on - strand, within LU632_RS12670 at 2410.792 kb on - strand, within LU632_RS12670 at 2410.796 kb on + strand, within LU632_RS12670 at 2410.829 kb on + strand, within LU632_RS12670 at 2410.829 kb on + strand, within LU632_RS12670 at 2410.830 kb on - strand, within LU632_RS12670 at 2410.830 kb on - strand, within LU632_RS12670 at 2410.832 kb on + strand, within LU632_RS12670 at 2410.833 kb on - strand, within LU632_RS12670 at 2410.896 kb on + strand, within LU632_RS12670 at 2410.919 kb on + strand at 2410.984 kb on + strand at 2411.046 kb on + strand at 2411.047 kb on - strand at 2411.047 kb on - strand at 2411.165 kb on + strand at 2411.166 kb on - strand at 2411.166 kb on - strand at 2411.202 kb on + strand, within LU632_RS12675 at 2411.203 kb on - strand, within LU632_RS12675 at 2411.203 kb on - strand, within LU632_RS12675 at 2411.265 kb on + strand, within LU632_RS12675 at 2411.266 kb on - strand, within LU632_RS12675 at 2411.281 kb on + strand, within LU632_RS12675 at 2411.329 kb on - strand, within LU632_RS12675 at 2411.359 kb on + strand, within LU632_RS12675 at 2411.382 kb on + strand, within LU632_RS12675 at 2411.382 kb on + strand, within LU632_RS12675 at 2411.383 kb on - strand, within LU632_RS12675 at 2411.525 kb on - strand, within LU632_RS12675 at 2411.550 kb on + strand, within LU632_RS12675 at 2411.563 kb on + strand, within LU632_RS12675 at 2411.606 kb on + strand, within LU632_RS12675 at 2411.606 kb on + strand, within LU632_RS12675 at 2411.606 kb on + strand, within LU632_RS12675 at 2411.673 kb on + strand, within LU632_RS12675 at 2412.023 kb on - strand, within LU632_RS12675 at 2412.085 kb on + strand, within LU632_RS12675 at 2412.085 kb on + strand, within LU632_RS12675 at 2412.355 kb on + strand, within LU632_RS12675 at 2412.356 kb on - strand, within LU632_RS12675 at 2412.356 kb on - strand, within LU632_RS12675 at 2412.407 kb on - strand, within LU632_RS12675 at 2412.424 kb on + strand, within LU632_RS12675 at 2412.424 kb on + strand, within LU632_RS12675 at 2412.424 kb on + strand, within LU632_RS12675 at 2412.425 kb on - strand, within LU632_RS12675 at 2412.425 kb on - strand, within LU632_RS12675 at 2412.615 kb on + strand, within LU632_RS12675 at 2412.645 kb on + strand, within LU632_RS12675 at 2412.645 kb on + strand, within LU632_RS12675 at 2412.649 kb on + strand, within LU632_RS12675 at 2412.649 kb on + strand, within LU632_RS12675 at 2412.650 kb on - strand, within LU632_RS12675 at 2412.660 kb on + strand, within LU632_RS12675 at 2412.661 kb on - strand, within LU632_RS12675 at 2412.745 kb on - strand, within LU632_RS12675 at 2412.747 kb on - strand, within LU632_RS12675 at 2412.747 kb on - strand, within LU632_RS12675 at 2412.747 kb on - strand, within LU632_RS12675 at 2412.783 kb on + strand, within LU632_RS12675 at 2412.811 kb on + strand, within LU632_RS12675 at 2412.811 kb on + strand, within LU632_RS12675 at 2412.901 kb on - strand, within LU632_RS12675 at 2412.909 kb on + strand, within LU632_RS12675 at 2412.992 kb on - strand, within LU632_RS12675 at 2413.024 kb on - strand, within LU632_RS12675 at 2413.026 kb on + strand, within LU632_RS12675 at 2413.026 kb on + strand, within LU632_RS12675 at 2413.053 kb on + strand, within LU632_RS12675 at 2413.150 kb on - strand, within LU632_RS12675 at 2413.150 kb on - strand, within LU632_RS12675 at 2413.181 kb on - strand, within LU632_RS12675 at 2413.185 kb on + strand, within LU632_RS12675 at 2413.185 kb on + strand, within LU632_RS12675 at 2413.185 kb on + strand, within LU632_RS12675 at 2413.186 kb on - strand, within LU632_RS12675 at 2413.186 kb on - strand, within LU632_RS12675 at 2413.219 kb on - strand, within LU632_RS12675 at 2413.376 kb on - strand at 2413.404 kb on + strand at 2413.404 kb on + strand at 2413.405 kb on - strand at 2413.408 kb on + strand at 2413.422 kb on + strand at 2413.422 kb on + strand at 2413.426 kb on - strand at 2413.438 kb on + strand at 2413.438 kb on + strand at 2413.439 kb on - strand at 2413.439 kb on - strand at 2413.477 kb on + strand at 2413.482 kb on - strand at 2413.576 kb on + strand at 2413.618 kb on + strand at 2413.681 kb on + strand at 2413.688 kb on - strand at 2413.876 kb on + strand, within LU632_RS12680 at 2413.880 kb on + strand, within LU632_RS12680 at 2413.880 kb on + strand, within LU632_RS12680 at 2413.881 kb on - strand, within LU632_RS12680 at 2413.895 kb on + strand, within LU632_RS12680 at 2413.911 kb on + strand, within LU632_RS12680 at 2413.968 kb on - strand, within LU632_RS12680 at 2414.065 kb on - strand, within LU632_RS12685 at 2414.093 kb on - strand, within LU632_RS12685 at 2414.152 kb on - strand, within LU632_RS12685 at 2414.291 kb on + strand, within LU632_RS12690 at 2414.292 kb on - strand, within LU632_RS12690 at 2414.343 kb on + strand, within LU632_RS12690 at 2414.485 kb on - strand, within LU632_RS12690
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,410,345 + +1.0 2,410,345 + -0.5 2,410,449 + LU632_RS12665 0.13 +0.4 2,410,449 + LU632_RS12665 0.13 -0.7 2,410,449 + LU632_RS12665 0.13 +0.8 2,410,450 + LU632_RS12665 0.13 -0.4 2,410,669 + LU632_RS12665 0.80 +1.0 2,410,669 + LU632_RS12665 0.80 -1.6 2,410,720 - -0.9 2,410,788 - LU632_RS12670 0.24 +0.6 2,410,792 - LU632_RS12670 0.26 +0.6 2,410,796 + LU632_RS12670 0.28 -0.1 2,410,829 + LU632_RS12670 0.46 +0.2 2,410,829 + LU632_RS12670 0.46 +0.0 2,410,830 - LU632_RS12670 0.46 -0.3 2,410,830 - LU632_RS12670 0.46 +0.2 2,410,832 + LU632_RS12670 0.47 +0.4 2,410,833 - LU632_RS12670 0.48 +0.0 2,410,896 + LU632_RS12670 0.81 +1.0 2,410,919 + +1.6 2,410,984 + -0.4 2,411,046 + -0.9 2,411,047 - +0.0 2,411,047 - +0.1 2,411,165 + +0.4 2,411,166 - -0.0 2,411,166 - +0.2 2,411,202 + LU632_RS12675 0.11 -0.7 2,411,203 - LU632_RS12675 0.11 -0.2 2,411,203 - LU632_RS12675 0.11 +0.6 2,411,265 + LU632_RS12675 0.13 +0.5 2,411,266 - LU632_RS12675 0.13 -0.8 2,411,281 + LU632_RS12675 0.14 +0.0 2,411,329 - LU632_RS12675 0.16 +0.2 2,411,359 + LU632_RS12675 0.17 -1.0 2,411,382 + LU632_RS12675 0.18 -1.0 2,411,382 + LU632_RS12675 0.18 +0.3 2,411,383 - LU632_RS12675 0.18 +0.6 2,411,525 - LU632_RS12675 0.23 +1.0 2,411,550 + LU632_RS12675 0.24 +0.3 2,411,563 + LU632_RS12675 0.25 +0.2 2,411,606 + LU632_RS12675 0.26 +1.0 2,411,606 + LU632_RS12675 0.26 +1.6 2,411,606 + LU632_RS12675 0.26 -0.0 2,411,673 + LU632_RS12675 0.29 +0.3 2,412,023 - LU632_RS12675 0.42 -0.0 2,412,085 + LU632_RS12675 0.44 +0.9 2,412,085 + LU632_RS12675 0.44 -0.4 2,412,355 + LU632_RS12675 0.55 -0.2 2,412,356 - LU632_RS12675 0.55 +0.1 2,412,356 - LU632_RS12675 0.55 +0.0 2,412,407 - LU632_RS12675 0.57 +0.6 2,412,424 + LU632_RS12675 0.57 +0.5 2,412,424 + LU632_RS12675 0.57 -0.5 2,412,424 + LU632_RS12675 0.57 +1.3 2,412,425 - LU632_RS12675 0.57 +0.1 2,412,425 - LU632_RS12675 0.57 +0.8 2,412,615 + LU632_RS12675 0.65 +0.4 2,412,645 + LU632_RS12675 0.66 +1.0 2,412,645 + LU632_RS12675 0.66 -1.3 2,412,649 + LU632_RS12675 0.66 +1.0 2,412,649 + LU632_RS12675 0.66 +0.5 2,412,650 - LU632_RS12675 0.66 +0.4 2,412,660 + LU632_RS12675 0.66 -0.1 2,412,661 - LU632_RS12675 0.66 -1.0 2,412,745 - LU632_RS12675 0.70 +0.3 2,412,747 - LU632_RS12675 0.70 +0.3 2,412,747 - LU632_RS12675 0.70 -0.3 2,412,747 - LU632_RS12675 0.70 -0.8 2,412,783 + LU632_RS12675 0.71 -0.2 2,412,811 + LU632_RS12675 0.72 -2.0 2,412,811 + LU632_RS12675 0.72 -1.0 2,412,901 - LU632_RS12675 0.75 +0.1 2,412,909 + LU632_RS12675 0.76 -1.6 2,412,992 - LU632_RS12675 0.79 -0.5 2,413,024 - LU632_RS12675 0.80 +1.0 2,413,026 + LU632_RS12675 0.80 +0.4 2,413,026 + LU632_RS12675 0.80 -0.1 2,413,053 + LU632_RS12675 0.81 -0.1 2,413,150 - LU632_RS12675 0.85 +0.1 2,413,150 - LU632_RS12675 0.85 +1.0 2,413,181 - LU632_RS12675 0.86 +0.1 2,413,185 + LU632_RS12675 0.86 -0.1 2,413,185 + LU632_RS12675 0.86 +0.2 2,413,185 + LU632_RS12675 0.86 -0.0 2,413,186 - LU632_RS12675 0.86 -1.7 2,413,186 - LU632_RS12675 0.86 +0.0 2,413,219 - LU632_RS12675 0.88 -0.5 2,413,376 - -0.6 2,413,404 + +1.0 2,413,404 + -0.9 2,413,405 - +0.3 2,413,408 + -0.4 2,413,422 + -0.1 2,413,422 + +0.2 2,413,426 - +1.0 2,413,438 + -0.5 2,413,438 + +0.5 2,413,439 - -0.8 2,413,439 - +0.9 2,413,477 + -0.1 2,413,482 - -0.2 2,413,576 + +1.2 2,413,618 + +0.3 2,413,681 + -0.5 2,413,688 - +0.2 2,413,876 + LU632_RS12680 0.32 +0.5 2,413,880 + LU632_RS12680 0.33 +0.4 2,413,880 + LU632_RS12680 0.33 +0.0 2,413,881 - LU632_RS12680 0.34 -0.5 2,413,895 + LU632_RS12680 0.39 +0.1 2,413,911 + LU632_RS12680 0.45 -0.2 2,413,968 - LU632_RS12680 0.67 +0.4 2,414,065 - LU632_RS12685 0.46 +0.8 2,414,093 - LU632_RS12685 0.60 +0.0 2,414,152 - LU632_RS12685 0.90 +0.0 2,414,291 + LU632_RS12690 0.17 -0.2 2,414,292 - LU632_RS12690 0.17 +0.2 2,414,343 + LU632_RS12690 0.26 -0.1 2,414,485 - LU632_RS12690 0.52 +0.3
Or see this region's nucleotide sequence