Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS12295 and bioD are separated by 47 nucleotides bioD and LU632_RS12305 are separated by 237 nucleotides LU632_RS12305 and LU632_RS12310 are separated by 128 nucleotides LU632_RS12310 and LU632_RS12315 are separated by 70 nucleotides
LU632_RS12295: LU632_RS12295 - ABC transporter ATP-binding protein, at 2,335,976 to 2,337,115
_RS12295
LU632_RS12300: bioD - dethiobiotin synthase, at 2,337,163 to 2,337,834
bioD
LU632_RS12305: LU632_RS12305 - ROK family transcriptional regulator, at 2,338,072 to 2,339,289
_RS12305
LU632_RS12310: LU632_RS12310 - hypothetical protein, at 2,339,418 to 2,339,585
_RS12310
LU632_RS12315: LU632_RS12315 - MFS transporter, at 2,339,656 to 2,340,771
_RS12315
Position (kb)
2338
2339
2340 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2337.072 kb on - strand at 2337.113 kb on + strand at 2337.114 kb on - strand at 2337.160 kb on + strand at 2337.194 kb on + strand at 2337.227 kb on - strand at 2337.267 kb on + strand, within bioD at 2337.282 kb on + strand, within bioD at 2337.297 kb on + strand, within bioD at 2337.298 kb on - strand, within bioD at 2337.312 kb on + strand, within bioD at 2337.313 kb on - strand, within bioD at 2337.313 kb on - strand, within bioD at 2337.409 kb on + strand, within bioD at 2337.550 kb on + strand, within bioD at 2337.550 kb on + strand, within bioD at 2337.659 kb on - strand, within bioD at 2337.664 kb on + strand, within bioD at 2337.676 kb on - strand, within bioD at 2337.821 kb on - strand at 2337.821 kb on - strand at 2337.904 kb on - strand at 2337.954 kb on - strand at 2338.109 kb on + strand at 2338.110 kb on - strand at 2338.124 kb on - strand at 2338.320 kb on + strand, within LU632_RS12305 at 2338.332 kb on + strand, within LU632_RS12305 at 2338.332 kb on + strand, within LU632_RS12305 at 2338.332 kb on + strand, within LU632_RS12305 at 2338.332 kb on + strand, within LU632_RS12305 at 2338.332 kb on + strand, within LU632_RS12305 at 2338.332 kb on + strand, within LU632_RS12305 at 2338.332 kb on + strand, within LU632_RS12305 at 2338.333 kb on - strand, within LU632_RS12305 at 2338.333 kb on - strand, within LU632_RS12305 at 2338.333 kb on - strand, within LU632_RS12305 at 2338.333 kb on - strand, within LU632_RS12305 at 2338.333 kb on - strand, within LU632_RS12305 at 2338.333 kb on - strand, within LU632_RS12305 at 2338.334 kb on + strand, within LU632_RS12305 at 2338.334 kb on + strand, within LU632_RS12305 at 2338.334 kb on + strand, within LU632_RS12305 at 2338.354 kb on - strand, within LU632_RS12305 at 2338.393 kb on + strand, within LU632_RS12305 at 2338.393 kb on + strand, within LU632_RS12305 at 2338.394 kb on - strand, within LU632_RS12305 at 2338.472 kb on - strand, within LU632_RS12305 at 2338.473 kb on + strand, within LU632_RS12305 at 2338.521 kb on - strand, within LU632_RS12305 at 2338.721 kb on + strand, within LU632_RS12305 at 2338.765 kb on + strand, within LU632_RS12305 at 2338.765 kb on + strand, within LU632_RS12305 at 2338.765 kb on + strand, within LU632_RS12305 at 2338.766 kb on - strand, within LU632_RS12305 at 2338.766 kb on - strand, within LU632_RS12305 at 2338.767 kb on + strand, within LU632_RS12305 at 2338.767 kb on + strand, within LU632_RS12305 at 2338.768 kb on - strand, within LU632_RS12305 at 2338.768 kb on - strand, within LU632_RS12305 at 2338.768 kb on - strand, within LU632_RS12305 at 2338.768 kb on - strand, within LU632_RS12305 at 2338.905 kb on + strand, within LU632_RS12305 at 2338.906 kb on - strand, within LU632_RS12305 at 2339.004 kb on + strand, within LU632_RS12305 at 2339.207 kb on + strand at 2339.303 kb on + strand at 2339.369 kb on + strand at 2339.370 kb on - strand at 2339.400 kb on - strand at 2339.400 kb on - strand at 2339.400 kb on - strand at 2339.401 kb on + strand at 2339.401 kb on + strand at 2339.402 kb on - strand at 2339.452 kb on - strand, within LU632_RS12310 at 2339.546 kb on + strand, within LU632_RS12310 at 2339.708 kb on + strand at 2339.764 kb on + strand at 2339.805 kb on + strand, within LU632_RS12315 at 2339.949 kb on - strand, within LU632_RS12315 at 2340.098 kb on + strand, within LU632_RS12315 at 2340.098 kb on + strand, within LU632_RS12315 at 2340.098 kb on + strand, within LU632_RS12315 at 2340.102 kb on + strand, within LU632_RS12315 at 2340.103 kb on - strand, within LU632_RS12315 at 2340.103 kb on - strand, within LU632_RS12315 at 2340.103 kb on - strand, within LU632_RS12315 at 2340.103 kb on - strand, within LU632_RS12315 at 2340.103 kb on - strand, within LU632_RS12315 at 2340.103 kb on - strand, within LU632_RS12315 at 2340.167 kb on - strand, within LU632_RS12315 at 2340.167 kb on - strand, within LU632_RS12315 at 2340.232 kb on + strand, within LU632_RS12315
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,337,072 - -0.0 2,337,113 + +1.0 2,337,114 - -0.4 2,337,160 + -0.5 2,337,194 + -0.2 2,337,227 - +0.5 2,337,267 + bioD LU632_RS12300 0.15 -0.6 2,337,282 + bioD LU632_RS12300 0.18 +0.9 2,337,297 + bioD LU632_RS12300 0.20 +0.1 2,337,298 - bioD LU632_RS12300 0.20 -1.3 2,337,312 + bioD LU632_RS12300 0.22 +0.2 2,337,313 - bioD LU632_RS12300 0.22 +0.4 2,337,313 - bioD LU632_RS12300 0.22 +0.0 2,337,409 + bioD LU632_RS12300 0.37 -0.0 2,337,550 + bioD LU632_RS12300 0.58 -0.6 2,337,550 + bioD LU632_RS12300 0.58 +0.1 2,337,659 - bioD LU632_RS12300 0.74 -0.3 2,337,664 + bioD LU632_RS12300 0.75 +0.3 2,337,676 - bioD LU632_RS12300 0.76 -0.6 2,337,821 - -0.3 2,337,821 - -1.3 2,337,904 - +0.3 2,337,954 - +0.0 2,338,109 + +1.6 2,338,110 - +0.2 2,338,124 - +0.0 2,338,320 + LU632_RS12305 0.20 +0.2 2,338,332 + LU632_RS12305 0.21 +0.4 2,338,332 + LU632_RS12305 0.21 +0.2 2,338,332 + LU632_RS12305 0.21 +0.4 2,338,332 + LU632_RS12305 0.21 +1.6 2,338,332 + LU632_RS12305 0.21 +0.1 2,338,332 + LU632_RS12305 0.21 +1.4 2,338,332 + LU632_RS12305 0.21 -0.7 2,338,333 - LU632_RS12305 0.21 +1.0 2,338,333 - LU632_RS12305 0.21 -0.3 2,338,333 - LU632_RS12305 0.21 +0.1 2,338,333 - LU632_RS12305 0.21 +0.2 2,338,333 - LU632_RS12305 0.21 -0.1 2,338,333 - LU632_RS12305 0.21 +0.1 2,338,334 + LU632_RS12305 0.22 -0.9 2,338,334 + LU632_RS12305 0.22 +0.0 2,338,334 + LU632_RS12305 0.22 +0.7 2,338,354 - LU632_RS12305 0.23 +1.3 2,338,393 + LU632_RS12305 0.26 -0.2 2,338,393 + LU632_RS12305 0.26 +0.1 2,338,394 - LU632_RS12305 0.26 -0.3 2,338,472 - LU632_RS12305 0.33 +0.4 2,338,473 + LU632_RS12305 0.33 +0.2 2,338,521 - LU632_RS12305 0.37 +1.0 2,338,721 + LU632_RS12305 0.53 +0.1 2,338,765 + LU632_RS12305 0.57 +0.1 2,338,765 + LU632_RS12305 0.57 +0.2 2,338,765 + LU632_RS12305 0.57 +0.8 2,338,766 - LU632_RS12305 0.57 +0.0 2,338,766 - LU632_RS12305 0.57 +0.3 2,338,767 + LU632_RS12305 0.57 +1.0 2,338,767 + LU632_RS12305 0.57 +0.3 2,338,768 - LU632_RS12305 0.57 +0.4 2,338,768 - LU632_RS12305 0.57 +0.4 2,338,768 - LU632_RS12305 0.57 -0.5 2,338,768 - LU632_RS12305 0.57 +0.0 2,338,905 + LU632_RS12305 0.68 -0.6 2,338,906 - LU632_RS12305 0.68 -0.1 2,339,004 + LU632_RS12305 0.77 -1.0 2,339,207 + -1.8 2,339,303 + +0.2 2,339,369 + +0.7 2,339,370 - +1.9 2,339,400 - +0.3 2,339,400 - +1.0 2,339,400 - -0.3 2,339,401 + +0.1 2,339,401 + -0.2 2,339,402 - +0.0 2,339,452 - LU632_RS12310 0.20 -0.0 2,339,546 + LU632_RS12310 0.76 -0.5 2,339,708 + +1.6 2,339,764 + +1.4 2,339,805 + LU632_RS12315 0.13 -0.1 2,339,949 - LU632_RS12315 0.26 -0.2 2,340,098 + LU632_RS12315 0.40 +0.3 2,340,098 + LU632_RS12315 0.40 -0.6 2,340,098 + LU632_RS12315 0.40 -0.7 2,340,102 + LU632_RS12315 0.40 -1.0 2,340,103 - LU632_RS12315 0.40 +0.5 2,340,103 - LU632_RS12315 0.40 +1.0 2,340,103 - LU632_RS12315 0.40 +0.3 2,340,103 - LU632_RS12315 0.40 -0.6 2,340,103 - LU632_RS12315 0.40 -0.7 2,340,103 - LU632_RS12315 0.40 -0.1 2,340,167 - LU632_RS12315 0.46 +0.6 2,340,167 - LU632_RS12315 0.46 -0.3 2,340,232 + LU632_RS12315 0.52 +0.5
Or see this region's nucleotide sequence