Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS11585 and LU632_RS11590 are separated by 506 nucleotides LU632_RS11590 and LU632_RS11595 are separated by 147 nucleotides LU632_RS11595 and LU632_RS11600 are separated by 31 nucleotides LU632_RS11600 and LU632_RS11605 are separated by 122 nucleotides
LU632_RS11585: LU632_RS11585 - IS3 family transposase, at 2,208,664 to 2,209,784
_RS11585
LU632_RS11590: LU632_RS11590 - hypothetical protein, at 2,210,291 to 2,210,479
_RS11590
LU632_RS11595: LU632_RS11595 - hypothetical protein, at 2,210,627 to 2,211,037
_RS11595
LU632_RS11600: LU632_RS11600 - carbohydrate binding domain-containing protein, at 2,211,069 to 2,211,482
_RS11600
LU632_RS11605: LU632_RS11605 - Rpn family recombination-promoting nuclease/putative transposase, at 2,211,605 to 2,212,141
_RS11605
Position (kb)
2210
2211
2212 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2209.791 kb on + strand at 2209.791 kb on + strand at 2209.791 kb on + strand at 2209.791 kb on + strand at 2209.795 kb on + strand at 2209.931 kb on + strand at 2209.931 kb on + strand at 2209.932 kb on - strand at 2210.017 kb on + strand at 2210.792 kb on - strand, within LU632_RS11595 at 2210.824 kb on + strand, within LU632_RS11595 at 2210.937 kb on + strand, within LU632_RS11595 at 2211.318 kb on - strand, within LU632_RS11600 at 2211.342 kb on - strand, within LU632_RS11600 at 2211.433 kb on + strand, within LU632_RS11600 at 2211.453 kb on - strand at 2211.455 kb on + strand at 2211.456 kb on - strand at 2211.456 kb on - strand at 2211.588 kb on + strand at 2211.592 kb on + strand at 2211.592 kb on + strand at 2211.883 kb on + strand, within LU632_RS11605 at 2211.884 kb on - strand, within LU632_RS11605 at 2211.896 kb on + strand, within LU632_RS11605 at 2211.897 kb on - strand, within LU632_RS11605 at 2211.898 kb on + strand, within LU632_RS11605 at 2211.898 kb on + strand, within LU632_RS11605 at 2211.898 kb on + strand, within LU632_RS11605 at 2211.899 kb on - strand, within LU632_RS11605 at 2211.900 kb on + strand, within LU632_RS11605 at 2211.901 kb on - strand, within LU632_RS11605 at 2211.997 kb on + strand, within LU632_RS11605 at 2211.998 kb on - strand, within LU632_RS11605
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,209,791 + +0.0 2,209,791 + -2.0 2,209,791 + +0.7 2,209,791 + +0.6 2,209,795 + +1.0 2,209,931 + +1.9 2,209,931 + +0.4 2,209,932 - +0.8 2,210,017 + -0.4 2,210,792 - LU632_RS11595 0.40 +0.2 2,210,824 + LU632_RS11595 0.48 +0.0 2,210,937 + LU632_RS11595 0.75 +0.8 2,211,318 - LU632_RS11600 0.60 +0.3 2,211,342 - LU632_RS11600 0.66 -1.8 2,211,433 + LU632_RS11600 0.88 +0.6 2,211,453 - -0.6 2,211,455 + -1.2 2,211,456 - -1.4 2,211,456 - -0.3 2,211,588 + +0.3 2,211,592 + +0.3 2,211,592 + +1.0 2,211,883 + LU632_RS11605 0.52 -0.2 2,211,884 - LU632_RS11605 0.52 -1.1 2,211,896 + LU632_RS11605 0.54 -2.3 2,211,897 - LU632_RS11605 0.54 +0.2 2,211,898 + LU632_RS11605 0.55 +1.4 2,211,898 + LU632_RS11605 0.55 +0.2 2,211,898 + LU632_RS11605 0.55 +0.5 2,211,899 - LU632_RS11605 0.55 +0.0 2,211,900 + LU632_RS11605 0.55 -0.3 2,211,901 - LU632_RS11605 0.55 -1.3 2,211,997 + LU632_RS11605 0.73 +0.0 2,211,998 - LU632_RS11605 0.73 -1.0
Or see this region's nucleotide sequence