Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliP and fliO overlap by 4 nucleotides fliO and fliN are separated by 1 nucleotides fliN and fliM overlap by 8 nucleotides fliM and fliL are separated by 4 nucleotides fliL and LU632_RS10550 are separated by 152 nucleotides
LU632_RS10525: fliP - flagellar type III secretion system pore protein FliP, at 2,012,328 to 2,013,062
fliP
LU632_RS10530: fliO - flagellar biosynthetic protein FliO, at 2,013,059 to 2,013,454
fliO
LU632_RS10535: fliN - flagellar motor switch protein FliN, at 2,013,456 to 2,013,860
fliN
LU632_RS10540: fliM - flagellar motor switch protein FliM, at 2,013,853 to 2,014,860
fliM
LU632_RS10545: fliL - flagellar basal body-associated protein FliL, at 2,014,865 to 2,015,347
fliL
LU632_RS10550: LU632_RS10550 - flagellar hook-length control protein FliK, at 2,015,500 to 2,016,825
_RS10550
Position (kb)
2013
2014
2015 Strain fitness (log2 ratio)
-1
0
1
2 at 2012.861 kb on + strand, within fliP at 2013.009 kb on + strand at 2013.009 kb on + strand at 2013.009 kb on + strand at 2013.010 kb on - strand at 2013.010 kb on - strand at 2013.034 kb on - strand at 2013.034 kb on - strand at 2013.053 kb on + strand at 2013.054 kb on - strand at 2013.082 kb on + strand at 2013.083 kb on - strand at 2013.086 kb on + strand at 2013.087 kb on - strand at 2013.172 kb on + strand, within fliO at 2013.173 kb on - strand, within fliO at 2013.269 kb on + strand, within fliO at 2013.335 kb on + strand, within fliO at 2013.336 kb on - strand, within fliO at 2013.349 kb on + strand, within fliO at 2013.349 kb on + strand, within fliO at 2013.349 kb on + strand, within fliO at 2013.349 kb on + strand, within fliO at 2013.350 kb on - strand, within fliO at 2013.362 kb on + strand, within fliO at 2013.448 kb on + strand at 2013.449 kb on - strand at 2013.449 kb on - strand at 2013.517 kb on + strand, within fliN at 2013.644 kb on + strand, within fliN at 2013.923 kb on + strand at 2013.924 kb on - strand at 2013.924 kb on - strand at 2013.924 kb on - strand at 2013.956 kb on - strand, within fliM at 2014.012 kb on + strand, within fliM at 2014.012 kb on + strand, within fliM at 2014.012 kb on + strand, within fliM at 2014.013 kb on - strand, within fliM at 2014.013 kb on - strand, within fliM at 2014.013 kb on - strand, within fliM at 2014.047 kb on + strand, within fliM at 2014.048 kb on + strand, within fliM at 2014.048 kb on - strand, within fliM at 2014.127 kb on + strand, within fliM at 2014.127 kb on + strand, within fliM at 2014.128 kb on - strand, within fliM at 2014.131 kb on + strand, within fliM at 2014.131 kb on + strand, within fliM at 2014.261 kb on - strand, within fliM at 2014.325 kb on + strand, within fliM at 2014.326 kb on - strand, within fliM at 2014.326 kb on - strand, within fliM at 2014.326 kb on - strand, within fliM at 2014.367 kb on + strand, within fliM at 2014.561 kb on - strand, within fliM at 2014.596 kb on - strand, within fliM at 2014.662 kb on + strand, within fliM at 2014.663 kb on - strand, within fliM at 2014.726 kb on - strand, within fliM at 2014.867 kb on - strand at 2014.951 kb on + strand, within fliL at 2015.075 kb on - strand, within fliL at 2015.109 kb on + strand, within fliL at 2015.109 kb on + strand, within fliL at 2015.109 kb on + strand, within fliL at 2015.109 kb on + strand, within fliL at 2015.109 kb on + strand, within fliL at 2015.110 kb on - strand, within fliL at 2015.276 kb on + strand, within fliL at 2015.276 kb on + strand, within fliL at 2015.276 kb on + strand, within fliL at 2015.280 kb on - strand, within fliL at 2015.282 kb on + strand, within fliL at 2015.314 kb on + strand at 2015.315 kb on - strand at 2015.347 kb on + strand at 2015.357 kb on - strand at 2015.444 kb on + strand at 2015.445 kb on - strand at 2015.512 kb on - strand at 2015.601 kb on + strand at 2015.607 kb on + strand at 2015.607 kb on + strand at 2015.607 kb on + strand at 2015.608 kb on - strand at 2015.608 kb on - strand at 2015.608 kb on - strand at 2015.623 kb on - strand at 2015.731 kb on + strand, within LU632_RS10550 at 2015.787 kb on + strand, within LU632_RS10550 at 2015.788 kb on - strand, within LU632_RS10550 at 2015.854 kb on + strand, within LU632_RS10550 at 2015.854 kb on + strand, within LU632_RS10550 at 2015.854 kb on + strand, within LU632_RS10550 at 2015.854 kb on + strand, within LU632_RS10550 at 2015.855 kb on - strand, within LU632_RS10550 at 2015.855 kb on - strand, within LU632_RS10550 at 2015.855 kb on - strand, within LU632_RS10550
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,012,861 + fliP LU632_RS10525 0.73 +0.3 2,013,009 + +0.1 2,013,009 + +1.6 2,013,009 + +0.2 2,013,010 - +0.0 2,013,010 - -0.0 2,013,034 - +0.6 2,013,034 - -0.5 2,013,053 + -0.2 2,013,054 - -1.0 2,013,082 + +0.6 2,013,083 - -0.4 2,013,086 + +1.0 2,013,087 - +0.3 2,013,172 + fliO LU632_RS10530 0.29 +0.0 2,013,173 - fliO LU632_RS10530 0.29 -0.0 2,013,269 + fliO LU632_RS10530 0.53 -0.1 2,013,335 + fliO LU632_RS10530 0.70 +0.9 2,013,336 - fliO LU632_RS10530 0.70 -0.2 2,013,349 + fliO LU632_RS10530 0.73 +1.5 2,013,349 + fliO LU632_RS10530 0.73 +0.1 2,013,349 + fliO LU632_RS10530 0.73 +1.8 2,013,349 + fliO LU632_RS10530 0.73 -1.0 2,013,350 - fliO LU632_RS10530 0.73 +0.9 2,013,362 + fliO LU632_RS10530 0.77 -0.3 2,013,448 + -0.1 2,013,449 - -0.2 2,013,449 - -0.4 2,013,517 + fliN LU632_RS10535 0.15 -1.3 2,013,644 + fliN LU632_RS10535 0.46 +0.2 2,013,923 + -1.0 2,013,924 - -0.8 2,013,924 - -0.1 2,013,924 - -0.5 2,013,956 - fliM LU632_RS10540 0.10 -0.8 2,014,012 + fliM LU632_RS10540 0.16 -0.0 2,014,012 + fliM LU632_RS10540 0.16 -1.1 2,014,012 + fliM LU632_RS10540 0.16 +1.9 2,014,013 - fliM LU632_RS10540 0.16 -0.2 2,014,013 - fliM LU632_RS10540 0.16 +0.0 2,014,013 - fliM LU632_RS10540 0.16 -0.1 2,014,047 + fliM LU632_RS10540 0.19 -0.3 2,014,048 + fliM LU632_RS10540 0.19 -0.7 2,014,048 - fliM LU632_RS10540 0.19 -0.9 2,014,127 + fliM LU632_RS10540 0.27 +0.4 2,014,127 + fliM LU632_RS10540 0.27 -0.5 2,014,128 - fliM LU632_RS10540 0.27 -0.2 2,014,131 + fliM LU632_RS10540 0.28 -1.3 2,014,131 + fliM LU632_RS10540 0.28 -0.2 2,014,261 - fliM LU632_RS10540 0.40 +0.7 2,014,325 + fliM LU632_RS10540 0.47 -0.2 2,014,326 - fliM LU632_RS10540 0.47 +0.3 2,014,326 - fliM LU632_RS10540 0.47 -0.4 2,014,326 - fliM LU632_RS10540 0.47 -0.0 2,014,367 + fliM LU632_RS10540 0.51 -0.3 2,014,561 - fliM LU632_RS10540 0.70 -0.5 2,014,596 - fliM LU632_RS10540 0.74 +0.6 2,014,662 + fliM LU632_RS10540 0.80 -0.8 2,014,663 - fliM LU632_RS10540 0.80 -0.5 2,014,726 - fliM LU632_RS10540 0.87 +0.7 2,014,867 - +0.5 2,014,951 + fliL LU632_RS10545 0.18 -0.1 2,015,075 - fliL LU632_RS10545 0.43 -0.5 2,015,109 + fliL LU632_RS10545 0.51 -0.7 2,015,109 + fliL LU632_RS10545 0.51 +0.2 2,015,109 + fliL LU632_RS10545 0.51 +1.8 2,015,109 + fliL LU632_RS10545 0.51 -0.2 2,015,109 + fliL LU632_RS10545 0.51 -0.5 2,015,110 - fliL LU632_RS10545 0.51 +0.1 2,015,276 + fliL LU632_RS10545 0.85 +0.1 2,015,276 + fliL LU632_RS10545 0.85 -1.0 2,015,276 + fliL LU632_RS10545 0.85 -0.2 2,015,280 - fliL LU632_RS10545 0.86 +1.0 2,015,282 + fliL LU632_RS10545 0.86 -0.2 2,015,314 + -0.6 2,015,315 - -0.8 2,015,347 + -1.0 2,015,357 - -0.2 2,015,444 + +1.4 2,015,445 - +0.1 2,015,512 - -0.3 2,015,601 + +1.3 2,015,607 + +1.0 2,015,607 + -0.3 2,015,607 + +0.5 2,015,608 - -1.0 2,015,608 - +0.8 2,015,608 - +1.0 2,015,623 - -0.3 2,015,731 + LU632_RS10550 0.17 -0.0 2,015,787 + LU632_RS10550 0.22 +0.7 2,015,788 - LU632_RS10550 0.22 -0.2 2,015,854 + LU632_RS10550 0.27 -1.3 2,015,854 + LU632_RS10550 0.27 +1.5 2,015,854 + LU632_RS10550 0.27 +1.6 2,015,854 + LU632_RS10550 0.27 +0.1 2,015,855 - LU632_RS10550 0.27 -0.3 2,015,855 - LU632_RS10550 0.27 +0.2 2,015,855 - LU632_RS10550 0.27 -0.3
Or see this region's nucleotide sequence