Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS10280 and LU632_RS10285 are separated by 6 nucleotides LU632_RS10285 and LU632_RS10290 are separated by 347 nucleotides LU632_RS10290 and LU632_RS10295 are separated by 274 nucleotides
LU632_RS10280: LU632_RS10280 - YceK/YidQ family lipoprotein, at 1,965,213 to 1,965,452
_RS10280
LU632_RS10285: LU632_RS10285 - MysB family protein, at 1,965,459 to 1,965,857
_RS10285
LU632_RS10290: LU632_RS10290 - DUF1440 domain-containing protein, at 1,966,205 to 1,966,762
_RS10290
LU632_RS10295: LU632_RS10295 - LpxL/LpxP family Kdo(2)-lipid IV(A) lauroyl/palmitoleoyl acyltransferasee, at 1,967,037 to 1,967,957
_RS10295
Position (kb)
1966
1967 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1965.262 kb on + strand, within LU632_RS10280 at 1965.262 kb on + strand, within LU632_RS10280 at 1965.262 kb on + strand, within LU632_RS10280 at 1965.262 kb on + strand, within LU632_RS10280 at 1965.263 kb on - strand, within LU632_RS10280 at 1965.263 kb on - strand, within LU632_RS10280 at 1965.281 kb on + strand, within LU632_RS10280 at 1965.282 kb on - strand, within LU632_RS10280 at 1965.324 kb on + strand, within LU632_RS10280 at 1965.377 kb on + strand, within LU632_RS10280 at 1965.453 kb on + strand at 1965.454 kb on - strand at 1965.454 kb on - strand at 1965.457 kb on + strand at 1965.457 kb on + strand at 1965.458 kb on - strand at 1965.466 kb on + strand at 1965.466 kb on + strand at 1965.466 kb on + strand at 1965.467 kb on - strand at 1965.483 kb on - strand at 1965.578 kb on + strand, within LU632_RS10285 at 1965.579 kb on - strand, within LU632_RS10285 at 1965.716 kb on + strand, within LU632_RS10285 at 1965.716 kb on + strand, within LU632_RS10285 at 1965.716 kb on + strand, within LU632_RS10285 at 1965.717 kb on - strand, within LU632_RS10285 at 1965.717 kb on - strand, within LU632_RS10285 at 1965.717 kb on - strand, within LU632_RS10285 at 1965.717 kb on - strand, within LU632_RS10285 at 1965.717 kb on - strand, within LU632_RS10285 at 1965.717 kb on - strand, within LU632_RS10285 at 1965.740 kb on - strand, within LU632_RS10285 at 1965.949 kb on + strand at 1966.130 kb on - strand at 1966.198 kb on + strand at 1966.198 kb on + strand at 1966.199 kb on - strand at 1966.199 kb on - strand at 1966.199 kb on - strand at 1966.202 kb on + strand at 1966.203 kb on - strand at 1966.203 kb on - strand at 1966.203 kb on - strand at 1966.203 kb on - strand at 1966.203 kb on - strand at 1966.286 kb on - strand, within LU632_RS10290 at 1966.316 kb on + strand, within LU632_RS10290 at 1966.316 kb on + strand, within LU632_RS10290 at 1966.316 kb on + strand, within LU632_RS10290 at 1966.421 kb on - strand, within LU632_RS10290 at 1966.453 kb on - strand, within LU632_RS10290 at 1966.462 kb on + strand, within LU632_RS10290 at 1966.492 kb on + strand, within LU632_RS10290 at 1966.524 kb on + strand, within LU632_RS10290 at 1966.524 kb on + strand, within LU632_RS10290 at 1966.524 kb on + strand, within LU632_RS10290 at 1966.525 kb on - strand, within LU632_RS10290 at 1966.629 kb on - strand, within LU632_RS10290 at 1966.683 kb on - strand, within LU632_RS10290 at 1966.726 kb on + strand at 1966.729 kb on + strand at 1966.729 kb on + strand at 1966.730 kb on - strand at 1966.730 kb on - strand at 1966.730 kb on - strand at 1966.730 kb on - strand at 1966.730 kb on - strand at 1966.740 kb on + strand at 1966.742 kb on + strand at 1966.742 kb on + strand at 1966.748 kb on + strand at 1966.960 kb on + strand at 1966.963 kb on - strand at 1967.093 kb on + strand at 1967.093 kb on + strand at 1967.093 kb on + strand at 1967.093 kb on + strand at 1967.093 kb on + strand at 1967.093 kb on + strand at 1967.093 kb on + strand at 1967.093 kb on + strand at 1967.093 kb on + strand at 1967.094 kb on - strand at 1967.094 kb on - strand at 1967.094 kb on - strand at 1967.094 kb on - strand at 1967.094 kb on - strand at 1967.094 kb on - strand at 1967.095 kb on + strand at 1967.095 kb on + strand at 1967.095 kb on + strand at 1967.095 kb on + strand at 1967.096 kb on - strand at 1967.096 kb on - strand at 1967.096 kb on - strand at 1967.096 kb on - strand at 1967.135 kb on + strand, within LU632_RS10295 at 1967.375 kb on + strand, within LU632_RS10295 at 1967.412 kb on + strand, within LU632_RS10295 at 1967.420 kb on + strand, within LU632_RS10295 at 1967.613 kb on - strand, within LU632_RS10295
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 1,965,262 + LU632_RS10280 0.20 +0.2 1,965,262 + LU632_RS10280 0.20 -0.8 1,965,262 + LU632_RS10280 0.20 -0.6 1,965,262 + LU632_RS10280 0.20 +0.7 1,965,263 - LU632_RS10280 0.21 +0.4 1,965,263 - LU632_RS10280 0.21 +0.0 1,965,281 + LU632_RS10280 0.28 +0.3 1,965,282 - LU632_RS10280 0.29 +0.4 1,965,324 + LU632_RS10280 0.46 -0.5 1,965,377 + LU632_RS10280 0.68 -0.2 1,965,453 + +0.0 1,965,454 - -1.0 1,965,454 - +0.7 1,965,457 + -1.1 1,965,457 + +1.0 1,965,458 - -0.5 1,965,466 + -0.3 1,965,466 + -0.4 1,965,466 + +1.2 1,965,467 - -0.3 1,965,483 - +0.6 1,965,578 + LU632_RS10285 0.30 +0.2 1,965,579 - LU632_RS10285 0.30 -1.1 1,965,716 + LU632_RS10285 0.64 +0.9 1,965,716 + LU632_RS10285 0.64 +1.0 1,965,716 + LU632_RS10285 0.64 +0.6 1,965,717 - LU632_RS10285 0.65 +0.1 1,965,717 - LU632_RS10285 0.65 -0.3 1,965,717 - LU632_RS10285 0.65 +0.6 1,965,717 - LU632_RS10285 0.65 +0.6 1,965,717 - LU632_RS10285 0.65 +0.3 1,965,717 - LU632_RS10285 0.65 +0.6 1,965,740 - LU632_RS10285 0.70 +0.2 1,965,949 + +0.4 1,966,130 - -0.2 1,966,198 + -0.3 1,966,198 + +0.6 1,966,199 - -0.3 1,966,199 - -0.3 1,966,199 - +0.2 1,966,202 + -0.0 1,966,203 - +0.2 1,966,203 - -0.4 1,966,203 - +0.4 1,966,203 - +0.3 1,966,203 - +0.2 1,966,286 - LU632_RS10290 0.15 +0.6 1,966,316 + LU632_RS10290 0.20 +0.5 1,966,316 + LU632_RS10290 0.20 +0.1 1,966,316 + LU632_RS10290 0.20 -0.1 1,966,421 - LU632_RS10290 0.39 +0.3 1,966,453 - LU632_RS10290 0.44 -0.2 1,966,462 + LU632_RS10290 0.46 +1.0 1,966,492 + LU632_RS10290 0.51 +0.0 1,966,524 + LU632_RS10290 0.57 -0.9 1,966,524 + LU632_RS10290 0.57 +0.3 1,966,524 + LU632_RS10290 0.57 +1.4 1,966,525 - LU632_RS10290 0.57 +0.4 1,966,629 - LU632_RS10290 0.76 -1.3 1,966,683 - LU632_RS10290 0.86 +0.0 1,966,726 + +0.2 1,966,729 + -0.1 1,966,729 + +1.8 1,966,730 - +0.4 1,966,730 - -1.3 1,966,730 - -0.7 1,966,730 - -0.7 1,966,730 - -1.0 1,966,740 + +2.0 1,966,742 + -0.1 1,966,742 + +0.6 1,966,748 + +0.3 1,966,960 + +0.4 1,966,963 - -0.3 1,967,093 + +0.0 1,967,093 + +0.0 1,967,093 + +0.2 1,967,093 + +0.4 1,967,093 + +0.0 1,967,093 + -1.0 1,967,093 + -1.1 1,967,093 + -1.4 1,967,093 + -0.4 1,967,094 - +0.1 1,967,094 - +0.6 1,967,094 - -0.2 1,967,094 - +0.6 1,967,094 - -1.5 1,967,094 - -1.1 1,967,095 + -0.3 1,967,095 + -0.4 1,967,095 + -2.8 1,967,095 + -0.5 1,967,096 - -0.4 1,967,096 - +0.8 1,967,096 - -1.4 1,967,096 - -1.2 1,967,135 + LU632_RS10295 0.11 -0.7 1,967,375 + LU632_RS10295 0.37 -1.2 1,967,412 + LU632_RS10295 0.41 -2.1 1,967,420 + LU632_RS10295 0.42 +0.0 1,967,613 - LU632_RS10295 0.63 -0.8
Or see this region's nucleotide sequence