Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS09335 and idi are separated by 140 nucleotides idi and LU632_RS09345 are separated by 90 nucleotides LU632_RS09345 and LU632_RS09350 are separated by 136 nucleotides LU632_RS09350 and LU632_RS09355 are separated by 81 nucleotides LU632_RS09355 and LU632_RS09360 overlap by 1 nucleotides
LU632_RS09335: LU632_RS09335 - methyl-accepting chemotaxis protein, at 1,766,922 to 1,768,817
_RS09335
LU632_RS09340: idi - isopentenyl-diphosphate Delta-isomerase, at 1,768,958 to 1,769,515
idi
LU632_RS09345: LU632_RS09345 - IS3 family transposase, at 1,769,606 to 1,770,770
_RS09345
LU632_RS09350: LU632_RS09350 - protein YohO, at 1,770,907 to 1,771,011
_RS09350
LU632_RS09355: LU632_RS09355 - DUF3833 domain-containing protein, at 1,771,093 to 1,771,626
_RS09355
LU632_RS09360: LU632_RS09360 - cyclopropane-fatty-acyl-phospholipid synthase family protein, at 1,771,626 to 1,772,861
_RS09360
Position (kb) 1769
1770
1771 Strain fitness (log2 ratio) -2
-1
0
1 at 1768.629 kb on - strand at 1768.629 kb on - strand at 1768.664 kb on + strand at 1768.693 kb on + strand at 1768.693 kb on + strand at 1768.856 kb on - strand at 1768.856 kb on - strand at 1768.929 kb on + strand at 1768.929 kb on + strand at 1768.929 kb on + strand at 1769.066 kb on + strand, within idi at 1769.117 kb on + strand, within idi at 1769.117 kb on + strand, within idi at 1769.118 kb on - strand, within idi at 1769.121 kb on + strand, within idi at 1769.121 kb on + strand, within idi at 1769.121 kb on + strand, within idi at 1769.122 kb on - strand, within idi at 1769.122 kb on - strand, within idi at 1769.122 kb on - strand, within idi at 1769.143 kb on + strand, within idi at 1769.187 kb on - strand, within idi at 1769.309 kb on + strand, within idi at 1769.309 kb on + strand, within idi at 1769.417 kb on + strand, within idi at 1769.418 kb on - strand, within idi at 1770.775 kb on + strand at 1770.837 kb on - strand at 1770.848 kb on + strand at 1770.849 kb on - strand at 1770.849 kb on - strand at 1770.929 kb on + strand, within LU632_RS09350 at 1771.012 kb on - strand at 1771.027 kb on - strand at 1771.052 kb on - strand at 1771.052 kb on - strand at 1771.052 kb on - strand at 1771.229 kb on + strand, within LU632_RS09355 at 1771.230 kb on - strand, within LU632_RS09355 at 1771.230 kb on - strand, within LU632_RS09355 at 1771.250 kb on - strand, within LU632_RS09355 at 1771.559 kb on + strand, within LU632_RS09355 at 1771.560 kb on - strand, within LU632_RS09355 at 1771.560 kb on - strand, within LU632_RS09355 at 1771.560 kb on - strand, within LU632_RS09355 at 1771.564 kb on - strand, within LU632_RS09355 at 1771.564 kb on - strand, within LU632_RS09355 at 1771.699 kb on + strand at 1771.708 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 1,768,629 - +1.0 1,768,629 - -0.3 1,768,664 + -0.3 1,768,693 + +0.1 1,768,693 + -0.6 1,768,856 - -0.4 1,768,856 - +0.2 1,768,929 + -0.2 1,768,929 + -0.4 1,768,929 + +0.0 1,769,066 + idi LU632_RS09340 0.19 -0.7 1,769,117 + idi LU632_RS09340 0.28 -0.0 1,769,117 + idi LU632_RS09340 0.28 -0.5 1,769,118 - idi LU632_RS09340 0.29 -0.5 1,769,121 + idi LU632_RS09340 0.29 +0.9 1,769,121 + idi LU632_RS09340 0.29 -0.6 1,769,121 + idi LU632_RS09340 0.29 +0.2 1,769,122 - idi LU632_RS09340 0.29 +0.5 1,769,122 - idi LU632_RS09340 0.29 -0.3 1,769,122 - idi LU632_RS09340 0.29 -0.4 1,769,143 + idi LU632_RS09340 0.33 +0.3 1,769,187 - idi LU632_RS09340 0.41 -0.3 1,769,309 + idi LU632_RS09340 0.63 +0.5 1,769,309 + idi LU632_RS09340 0.63 -1.3 1,769,417 + idi LU632_RS09340 0.82 -0.4 1,769,418 - idi LU632_RS09340 0.82 +0.7 1,770,775 + +1.5 1,770,837 - -0.5 1,770,848 + -0.0 1,770,849 - -0.3 1,770,849 - -0.3 1,770,929 + LU632_RS09350 0.21 +1.2 1,771,012 - -1.0 1,771,027 - -0.8 1,771,052 - +1.6 1,771,052 - +0.0 1,771,052 - +0.3 1,771,229 + LU632_RS09355 0.25 +0.5 1,771,230 - LU632_RS09355 0.26 -0.2 1,771,230 - LU632_RS09355 0.26 -0.1 1,771,250 - LU632_RS09355 0.29 +0.3 1,771,559 + LU632_RS09355 0.87 +0.0 1,771,560 - LU632_RS09355 0.87 +0.5 1,771,560 - LU632_RS09355 0.87 +0.5 1,771,560 - LU632_RS09355 0.87 -0.5 1,771,564 - LU632_RS09355 0.88 +1.3 1,771,564 - LU632_RS09355 0.88 -2.0 1,771,699 + -0.1 1,771,708 + -0.3
Or see this region's nucleotide sequence