Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapA and bamC are separated by 15 nucleotides bamC and LU632_RS08335 are separated by 445 nucleotides
LU632_RS08325: dapA - 4-hydroxy-tetrahydrodipicolinate synthase, at 1,565,861 to 1,566,739
dapA
LU632_RS08330: bamC - outer membrane protein assembly factor BamC, at 1,566,755 to 1,567,789
bamC
LU632_RS08335: LU632_RS08335 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 1,568,235 to 1,568,948
_RS08335
Position (kb)
1566
1567
1568 Strain fitness (log2 ratio)
-2
-1
0
1 at 1565.756 kb on - strand at 1565.761 kb on + strand at 1566.737 kb on + strand at 1566.738 kb on - strand at 1566.761 kb on + strand at 1566.845 kb on + strand at 1566.845 kb on + strand at 1566.847 kb on + strand at 1566.847 kb on + strand at 1566.847 kb on + strand at 1566.848 kb on - strand at 1567.058 kb on - strand, within bamC at 1567.058 kb on - strand, within bamC at 1567.139 kb on + strand, within bamC at 1567.139 kb on + strand, within bamC at 1567.141 kb on + strand, within bamC at 1567.141 kb on + strand, within bamC at 1567.141 kb on + strand, within bamC at 1567.263 kb on - strand, within bamC at 1567.297 kb on - strand, within bamC at 1567.384 kb on + strand, within bamC at 1567.385 kb on - strand, within bamC at 1567.474 kb on + strand, within bamC at 1567.544 kb on - strand, within bamC at 1567.592 kb on + strand, within bamC at 1567.592 kb on + strand, within bamC at 1567.593 kb on - strand, within bamC at 1567.595 kb on - strand, within bamC at 1567.595 kb on - strand, within bamC at 1567.726 kb on + strand at 1567.726 kb on + strand at 1567.727 kb on - strand at 1567.956 kb on + strand at 1568.027 kb on + strand at 1568.027 kb on + strand at 1568.028 kb on - strand at 1568.053 kb on - strand at 1568.077 kb on + strand at 1568.187 kb on - strand at 1568.192 kb on - strand at 1568.192 kb on - strand at 1568.509 kb on + strand, within LU632_RS08335 at 1568.631 kb on - strand, within LU632_RS08335 at 1568.640 kb on + strand, within LU632_RS08335 at 1568.685 kb on - strand, within LU632_RS08335
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 1,565,756 - -0.4 1,565,761 + +0.3 1,566,737 + +0.3 1,566,738 - +0.2 1,566,761 + +1.1 1,566,845 + +0.2 1,566,845 + -1.5 1,566,847 + -0.4 1,566,847 + -0.3 1,566,847 + -0.9 1,566,848 - +1.6 1,567,058 - bamC LU632_RS08330 0.29 +0.1 1,567,058 - bamC LU632_RS08330 0.29 +1.6 1,567,139 + bamC LU632_RS08330 0.37 -0.1 1,567,139 + bamC LU632_RS08330 0.37 -0.6 1,567,141 + bamC LU632_RS08330 0.37 +0.1 1,567,141 + bamC LU632_RS08330 0.37 -2.1 1,567,141 + bamC LU632_RS08330 0.37 -0.5 1,567,263 - bamC LU632_RS08330 0.49 -0.2 1,567,297 - bamC LU632_RS08330 0.52 +0.2 1,567,384 + bamC LU632_RS08330 0.61 -0.9 1,567,385 - bamC LU632_RS08330 0.61 -0.4 1,567,474 + bamC LU632_RS08330 0.69 +0.1 1,567,544 - bamC LU632_RS08330 0.76 +1.5 1,567,592 + bamC LU632_RS08330 0.81 +0.1 1,567,592 + bamC LU632_RS08330 0.81 +1.5 1,567,593 - bamC LU632_RS08330 0.81 +0.5 1,567,595 - bamC LU632_RS08330 0.81 +0.4 1,567,595 - bamC LU632_RS08330 0.81 +1.4 1,567,726 + -0.5 1,567,726 + -0.6 1,567,727 - +0.5 1,567,956 + -0.5 1,568,027 + -0.0 1,568,027 + -0.2 1,568,028 - +1.5 1,568,053 - +0.5 1,568,077 + -0.6 1,568,187 - -0.3 1,568,192 - -0.4 1,568,192 - -0.6 1,568,509 + LU632_RS08335 0.38 -0.4 1,568,631 - LU632_RS08335 0.55 -1.8 1,568,640 + LU632_RS08335 0.57 +0.1 1,568,685 - LU632_RS08335 0.63 -0.9
Or see this region's nucleotide sequence