Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apbE and alkB are separated by 21 nucleotides alkB and LU632_RS08200 are separated by 349 nucleotides
LU632_RS08190: apbE - FAD:protein FMN transferase ApbE, at 1,542,902 to 1,543,942
apbE
LU632_RS08195: alkB - DNA oxidative demethylase AlkB, at 1,543,964 to 1,544,607
alkB
LU632_RS08200: LU632_RS08200 - 4'-phosphopantetheinyl transferase superfamily protein, at 1,544,957 to 1,545,733
_RS08200
Position (kb)
1543
1544
1545 Strain fitness (log2 ratio)
-1
0
1
2 at 1543.208 kb on - strand, within apbE at 1543.259 kb on - strand, within apbE at 1543.315 kb on - strand, within apbE at 1543.374 kb on + strand, within apbE at 1543.550 kb on - strand, within apbE at 1543.674 kb on - strand, within apbE at 1543.735 kb on - strand, within apbE at 1543.941 kb on - strand at 1543.963 kb on + strand at 1543.964 kb on - strand at 1543.978 kb on + strand at 1544.112 kb on + strand, within alkB at 1544.305 kb on + strand, within alkB at 1544.306 kb on - strand, within alkB at 1544.306 kb on - strand, within alkB at 1544.333 kb on - strand, within alkB at 1544.347 kb on - strand, within alkB at 1544.421 kb on - strand, within alkB at 1544.421 kb on - strand, within alkB at 1544.442 kb on - strand, within alkB at 1544.485 kb on - strand, within alkB at 1544.513 kb on + strand, within alkB at 1544.578 kb on - strand at 1544.633 kb on + strand at 1544.735 kb on + strand at 1544.735 kb on + strand at 1544.736 kb on - strand at 1544.736 kb on - strand at 1544.807 kb on - strand at 1544.814 kb on - strand at 1544.834 kb on + strand at 1544.834 kb on + strand at 1544.835 kb on - strand at 1544.855 kb on + strand at 1544.855 kb on + strand at 1544.881 kb on + strand at 1544.881 kb on + strand at 1544.913 kb on + strand at 1544.935 kb on + strand at 1544.935 kb on + strand at 1545.007 kb on + strand at 1545.008 kb on - strand at 1545.227 kb on + strand, within LU632_RS08200 at 1545.228 kb on - strand, within LU632_RS08200 at 1545.228 kb on - strand, within LU632_RS08200 at 1545.228 kb on - strand, within LU632_RS08200 at 1545.228 kb on - strand, within LU632_RS08200 at 1545.289 kb on + strand, within LU632_RS08200 at 1545.290 kb on - strand, within LU632_RS08200 at 1545.365 kb on - strand, within LU632_RS08200 at 1545.473 kb on + strand, within LU632_RS08200 at 1545.578 kb on + strand, within LU632_RS08200
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 1,543,208 - apbE LU632_RS08190 0.29 +1.1 1,543,259 - apbE LU632_RS08190 0.34 -0.9 1,543,315 - apbE LU632_RS08190 0.40 +0.1 1,543,374 + apbE LU632_RS08190 0.45 +1.1 1,543,550 - apbE LU632_RS08190 0.62 +0.1 1,543,674 - apbE LU632_RS08190 0.74 -0.9 1,543,735 - apbE LU632_RS08190 0.80 -0.5 1,543,941 - -1.3 1,543,963 + -0.3 1,543,964 - +0.1 1,543,978 + +0.1 1,544,112 + alkB LU632_RS08195 0.23 -0.2 1,544,305 + alkB LU632_RS08195 0.53 -1.5 1,544,306 - alkB LU632_RS08195 0.53 -0.1 1,544,306 - alkB LU632_RS08195 0.53 -0.3 1,544,333 - alkB LU632_RS08195 0.57 +0.2 1,544,347 - alkB LU632_RS08195 0.59 +0.1 1,544,421 - alkB LU632_RS08195 0.71 +0.1 1,544,421 - alkB LU632_RS08195 0.71 +0.6 1,544,442 - alkB LU632_RS08195 0.74 +0.6 1,544,485 - alkB LU632_RS08195 0.81 -1.5 1,544,513 + alkB LU632_RS08195 0.85 +0.2 1,544,578 - -0.3 1,544,633 + +2.1 1,544,735 + +0.3 1,544,735 + -0.0 1,544,736 - +0.3 1,544,736 - -0.0 1,544,807 - +0.2 1,544,814 - -0.0 1,544,834 + +0.1 1,544,834 + +0.0 1,544,835 - -0.0 1,544,855 + -0.2 1,544,855 + -0.6 1,544,881 + -0.1 1,544,881 + +1.1 1,544,913 + +0.1 1,544,935 + -0.9 1,544,935 + +0.2 1,545,007 + -0.4 1,545,008 - -0.1 1,545,227 + LU632_RS08200 0.35 +0.1 1,545,228 - LU632_RS08200 0.35 -0.8 1,545,228 - LU632_RS08200 0.35 -0.3 1,545,228 - LU632_RS08200 0.35 -0.2 1,545,228 - LU632_RS08200 0.35 -0.5 1,545,289 + LU632_RS08200 0.43 -0.2 1,545,290 - LU632_RS08200 0.43 +0.6 1,545,365 - LU632_RS08200 0.53 +0.8 1,545,473 + LU632_RS08200 0.66 +0.1 1,545,578 + LU632_RS08200 0.80 +0.5
Or see this region's nucleotide sequence