Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menB and menC overlap by 1 nucleotides menC and menE overlap by 13 nucleotides menE and LU632_RS08130 are separated by 285 nucleotides
LU632_RS08115: menB - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 1,518,963 to 1,519,820
menB
LU632_RS08120: menC - o-succinylbenzoate synthase, at 1,519,820 to 1,520,791
menC
LU632_RS08125: menE - o-succinylbenzoate--CoA ligase, at 1,520,779 to 1,522,149
menE
LU632_RS08130: LU632_RS08130 - nicotinamide mononucleotide deamidase-related protein YfaY, at 1,522,435 to 1,523,640
_RS08130
Position (kb)
1520
1521
1522
1523 Strain fitness (log2 ratio)
-1
0
1
2 at 1519.845 kb on - strand at 1520.231 kb on + strand, within menC at 1520.231 kb on + strand, within menC at 1520.231 kb on + strand, within menC at 1520.232 kb on - strand, within menC at 1520.232 kb on - strand, within menC at 1520.232 kb on - strand, within menC at 1520.232 kb on - strand, within menC at 1520.368 kb on + strand, within menC at 1520.404 kb on + strand, within menC at 1520.778 kb on + strand at 1520.929 kb on + strand, within menE at 1520.930 kb on - strand, within menE at 1520.949 kb on - strand, within menE at 1520.986 kb on + strand, within menE at 1520.997 kb on - strand, within menE at 1521.099 kb on - strand, within menE at 1521.236 kb on + strand, within menE at 1521.237 kb on - strand, within menE at 1521.237 kb on - strand, within menE at 1521.240 kb on + strand, within menE at 1521.240 kb on + strand, within menE at 1521.287 kb on + strand, within menE at 1521.402 kb on + strand, within menE at 1521.403 kb on - strand, within menE at 1521.432 kb on + strand, within menE at 1521.512 kb on + strand, within menE at 1521.513 kb on - strand, within menE at 1521.516 kb on - strand, within menE at 1521.680 kb on + strand, within menE at 1521.945 kb on - strand, within menE at 1521.945 kb on - strand, within menE at 1522.052 kb on + strand at 1522.290 kb on + strand at 1522.338 kb on + strand at 1522.338 kb on + strand at 1522.395 kb on + strand at 1522.395 kb on + strand at 1522.396 kb on - strand at 1522.399 kb on + strand at 1522.399 kb on + strand at 1522.400 kb on - strand at 1522.400 kb on - strand at 1522.400 kb on - strand at 1522.400 kb on - strand at 1522.400 kb on - strand at 1522.400 kb on - strand at 1522.400 kb on - strand at 1522.400 kb on - strand at 1522.405 kb on + strand at 1522.853 kb on + strand, within LU632_RS08130 at 1522.853 kb on + strand, within LU632_RS08130 at 1522.854 kb on - strand, within LU632_RS08130 at 1522.953 kb on + strand, within LU632_RS08130
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 1,519,845 - +0.1 1,520,231 + menC LU632_RS08120 0.42 -0.5 1,520,231 + menC LU632_RS08120 0.42 +0.7 1,520,231 + menC LU632_RS08120 0.42 +0.1 1,520,232 - menC LU632_RS08120 0.42 -0.3 1,520,232 - menC LU632_RS08120 0.42 +0.4 1,520,232 - menC LU632_RS08120 0.42 -0.1 1,520,232 - menC LU632_RS08120 0.42 +0.1 1,520,368 + menC LU632_RS08120 0.56 +0.3 1,520,404 + menC LU632_RS08120 0.60 -0.1 1,520,778 + +0.2 1,520,929 + menE LU632_RS08125 0.11 -0.1 1,520,930 - menE LU632_RS08125 0.11 +0.3 1,520,949 - menE LU632_RS08125 0.12 -0.8 1,520,986 + menE LU632_RS08125 0.15 -1.0 1,520,997 - menE LU632_RS08125 0.16 -0.2 1,521,099 - menE LU632_RS08125 0.23 +0.2 1,521,236 + menE LU632_RS08125 0.33 +0.1 1,521,237 - menE LU632_RS08125 0.33 +0.6 1,521,237 - menE LU632_RS08125 0.33 +1.1 1,521,240 + menE LU632_RS08125 0.34 +0.4 1,521,240 + menE LU632_RS08125 0.34 +0.9 1,521,287 + menE LU632_RS08125 0.37 -0.0 1,521,402 + menE LU632_RS08125 0.45 -0.0 1,521,403 - menE LU632_RS08125 0.46 +1.1 1,521,432 + menE LU632_RS08125 0.48 +0.1 1,521,512 + menE LU632_RS08125 0.53 -0.0 1,521,513 - menE LU632_RS08125 0.54 +2.6 1,521,516 - menE LU632_RS08125 0.54 -0.3 1,521,680 + menE LU632_RS08125 0.66 -0.9 1,521,945 - menE LU632_RS08125 0.85 +0.1 1,521,945 - menE LU632_RS08125 0.85 +0.1 1,522,052 + +0.3 1,522,290 + +0.6 1,522,338 + +0.1 1,522,338 + -0.5 1,522,395 + -0.4 1,522,395 + -0.9 1,522,396 - -0.5 1,522,399 + +0.3 1,522,399 + +0.2 1,522,400 - +0.6 1,522,400 - +0.7 1,522,400 - +0.7 1,522,400 - +0.3 1,522,400 - -0.3 1,522,400 - +0.4 1,522,400 - +0.1 1,522,400 - -1.1 1,522,405 + -0.5 1,522,853 + LU632_RS08130 0.35 -0.3 1,522,853 + LU632_RS08130 0.35 -0.5 1,522,854 - LU632_RS08130 0.35 -0.8 1,522,953 + LU632_RS08130 0.43 -0.8
Or see this region's nucleotide sequence