Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS04710 and LU632_RS04715 are separated by 350 nucleotides LU632_RS04715 and LU632_RS04720 are separated by 207 nucleotides LU632_RS04720 and LU632_RS04725 overlap by 14 nucleotides
LU632_RS04710: LU632_RS04710 - IS110 family transposase, at 923,362 to 924,359
_RS04710
LU632_RS04715: LU632_RS04715 - IS5 family transposase, at 924,710 to 925,477
_RS04715
LU632_RS04720: LU632_RS04720 - response regulator transcription factor, at 925,685 to 926,320
_RS04720
LU632_RS04725: LU632_RS04725 - transporter substrate-binding domain-containing protein, at 926,307 to 929,459
_RS04725
Position (kb)
924
925
926 Strain fitness (log2 ratio)
-2
-1
0
1 at 923.907 kb on - strand, within LU632_RS04710 at 924.491 kb on - strand at 924.491 kb on - strand at 924.491 kb on - strand at 924.516 kb on - strand at 924.516 kb on - strand at 924.568 kb on + strand at 924.569 kb on - strand at 924.590 kb on + strand at 924.591 kb on - strand at 924.591 kb on - strand at 924.597 kb on + strand at 924.598 kb on - strand at 924.599 kb on + strand at 924.600 kb on - strand at 924.601 kb on + strand at 924.602 kb on - strand at 924.629 kb on + strand at 924.630 kb on - strand at 924.665 kb on + strand at 924.666 kb on - strand at 924.666 kb on - strand at 924.672 kb on + strand at 924.673 kb on - strand at 924.674 kb on + strand at 924.674 kb on + strand at 924.675 kb on - strand at 924.675 kb on - strand at 924.689 kb on + strand at 924.708 kb on + strand at 924.728 kb on + strand at 924.752 kb on - strand at 924.854 kb on - strand, within LU632_RS04715 at 924.854 kb on - strand, within LU632_RS04715 at 924.958 kb on + strand, within LU632_RS04715 at 924.959 kb on - strand, within LU632_RS04715 at 924.963 kb on - strand, within LU632_RS04715 at 925.119 kb on + strand, within LU632_RS04715 at 925.119 kb on - strand, within LU632_RS04715 at 925.120 kb on - strand, within LU632_RS04715 at 925.142 kb on - strand, within LU632_RS04715 at 925.338 kb on - strand, within LU632_RS04715 at 925.391 kb on - strand, within LU632_RS04715 at 925.413 kb on - strand at 925.500 kb on - strand at 925.500 kb on - strand at 925.500 kb on - strand at 925.500 kb on - strand at 925.501 kb on + strand at 925.501 kb on + strand at 925.501 kb on + strand at 925.501 kb on + strand at 925.501 kb on + strand at 925.502 kb on + strand at 925.502 kb on - strand at 925.549 kb on - strand at 925.576 kb on + strand at 925.577 kb on - strand at 925.577 kb on - strand at 925.600 kb on + strand at 925.600 kb on + strand at 925.600 kb on + strand at 925.600 kb on + strand at 925.601 kb on - strand at 925.601 kb on - strand at 925.601 kb on - strand at 925.628 kb on + strand at 925.628 kb on + strand at 925.628 kb on + strand at 925.629 kb on - strand at 925.631 kb on - strand at 925.631 kb on - strand at 925.684 kb on + strand at 925.684 kb on + strand at 925.685 kb on - strand at 925.685 kb on - strand at 925.762 kb on + strand, within LU632_RS04720 at 925.774 kb on + strand, within LU632_RS04720 at 925.781 kb on - strand, within LU632_RS04720 at 925.781 kb on - strand, within LU632_RS04720 at 925.886 kb on - strand, within LU632_RS04720 at 925.906 kb on + strand, within LU632_RS04720 at 925.906 kb on + strand, within LU632_RS04720 at 925.942 kb on - strand, within LU632_RS04720 at 925.943 kb on + strand, within LU632_RS04720 at 925.982 kb on - strand, within LU632_RS04720 at 926.032 kb on + strand, within LU632_RS04720 at 926.032 kb on + strand, within LU632_RS04720 at 926.212 kb on + strand, within LU632_RS04720 at 926.213 kb on - strand, within LU632_RS04720 at 926.228 kb on + strand, within LU632_RS04720 at 926.228 kb on + strand, within LU632_RS04720 at 926.228 kb on + strand, within LU632_RS04720 at 926.229 kb on - strand, within LU632_RS04720 at 926.229 kb on - strand, within LU632_RS04720 at 926.230 kb on + strand, within LU632_RS04720 at 926.278 kb on + strand at 926.336 kb on - strand at 926.381 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 923,907 - LU632_RS04710 0.55 -0.6 924,491 - -2.0 924,491 - -0.4 924,491 - -0.0 924,516 - +0.1 924,516 - -0.1 924,568 + +0.0 924,569 - +0.3 924,590 + -0.6 924,591 - +0.6 924,591 - -0.2 924,597 + +0.1 924,598 - -1.8 924,599 + -1.0 924,600 - -0.6 924,601 + -0.3 924,602 - -0.5 924,629 + -0.3 924,630 - +0.1 924,665 + -0.9 924,666 - -0.8 924,666 - -0.4 924,672 + +0.5 924,673 - +0.0 924,674 + +0.7 924,674 + -0.5 924,675 - -0.3 924,675 - -0.2 924,689 + +0.2 924,708 + +0.1 924,728 + -0.1 924,752 - -0.8 924,854 - LU632_RS04715 0.19 +0.2 924,854 - LU632_RS04715 0.19 +0.2 924,958 + LU632_RS04715 0.32 -0.6 924,959 - LU632_RS04715 0.32 -0.4 924,963 - LU632_RS04715 0.33 -0.1 925,119 + LU632_RS04715 0.53 +0.2 925,119 - LU632_RS04715 0.53 -0.3 925,120 - LU632_RS04715 0.53 -0.4 925,142 - LU632_RS04715 0.56 +0.0 925,338 - LU632_RS04715 0.82 -0.9 925,391 - LU632_RS04715 0.89 -0.5 925,413 - +0.6 925,500 - +0.2 925,500 - -0.2 925,500 - +0.6 925,500 - +1.0 925,501 + -0.0 925,501 + +0.7 925,501 + -0.2 925,501 + -0.8 925,501 + +0.4 925,502 + -0.9 925,502 - +0.5 925,549 - -0.5 925,576 + -0.4 925,577 - -0.5 925,577 - -0.4 925,600 + +0.4 925,600 + -0.4 925,600 + -0.3 925,600 + -0.3 925,601 - -0.0 925,601 - -0.4 925,601 - -0.5 925,628 + -0.0 925,628 + -1.0 925,628 + +0.1 925,629 - +0.6 925,631 - -0.3 925,631 - +0.2 925,684 + +0.8 925,684 + +0.2 925,685 - -0.5 925,685 - -0.4 925,762 + LU632_RS04720 0.12 -0.1 925,774 + LU632_RS04720 0.14 -0.0 925,781 - LU632_RS04720 0.15 -0.6 925,781 - LU632_RS04720 0.15 +0.4 925,886 - LU632_RS04720 0.32 -0.1 925,906 + LU632_RS04720 0.35 -0.5 925,906 + LU632_RS04720 0.35 +0.3 925,942 - LU632_RS04720 0.40 +0.5 925,943 + LU632_RS04720 0.41 -0.8 925,982 - LU632_RS04720 0.47 -0.4 926,032 + LU632_RS04720 0.55 +0.3 926,032 + LU632_RS04720 0.55 +0.0 926,212 + LU632_RS04720 0.83 +0.5 926,213 - LU632_RS04720 0.83 +0.4 926,228 + LU632_RS04720 0.85 +0.2 926,228 + LU632_RS04720 0.85 -0.1 926,228 + LU632_RS04720 0.85 +0.8 926,229 - LU632_RS04720 0.86 +0.0 926,229 - LU632_RS04720 0.86 +0.2 926,230 + LU632_RS04720 0.86 -0.4 926,278 + -0.6 926,336 - +0.7 926,381 + -0.1
Or see this region's nucleotide sequence