Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS04670 and LU632_RS04675 overlap by 4 nucleotides LU632_RS04675 and LU632_RS04680 are separated by 248 nucleotides LU632_RS04680 and LU632_RS04685 are separated by 34 nucleotides LU632_RS04685 and LU632_RS04690 are separated by 6 nucleotides
LU632_RS04670: LU632_RS04670 - conjugative transfer ATPase, at 914,918 to 916,131
_RS04670
LU632_RS04675: LU632_RS04675 - hypothetical protein, at 916,128 to 916,532
_RS04675
LU632_RS04680: LU632_RS04680 - IS481 family transposase, at 916,781 to 917,689
_RS04680
LU632_RS04685: LU632_RS04685 - universal stress protein, at 917,724 to 918,089
_RS04685
LU632_RS04690: LU632_RS04690 - IclR family transcriptional regulator, at 918,096 to 918,878
_RS04690
Position (kb)
916
917
918 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 916.721 kb on + strand at 916.722 kb on - strand at 917.744 kb on + strand at 917.811 kb on + strand, within LU632_RS04685 at 917.811 kb on + strand, within LU632_RS04685 at 917.938 kb on - strand, within LU632_RS04685 at 917.938 kb on - strand, within LU632_RS04685 at 917.985 kb on + strand, within LU632_RS04685 at 917.985 kb on + strand, within LU632_RS04685 at 917.985 kb on + strand, within LU632_RS04685 at 917.985 kb on + strand, within LU632_RS04685 at 917.986 kb on - strand, within LU632_RS04685 at 917.986 kb on - strand, within LU632_RS04685 at 917.992 kb on - strand, within LU632_RS04685 at 918.120 kb on + strand at 918.207 kb on + strand, within LU632_RS04690 at 918.231 kb on + strand, within LU632_RS04690 at 918.353 kb on + strand, within LU632_RS04690 at 918.354 kb on - strand, within LU632_RS04690 at 918.508 kb on + strand, within LU632_RS04690 at 918.508 kb on + strand, within LU632_RS04690 at 918.508 kb on + strand, within LU632_RS04690 at 918.508 kb on + strand, within LU632_RS04690 at 918.509 kb on - strand, within LU632_RS04690 at 918.509 kb on - strand, within LU632_RS04690 at 918.640 kb on - strand, within LU632_RS04690
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 916,721 + -0.0 916,722 - -0.8 917,744 + +0.6 917,811 + LU632_RS04685 0.24 -1.5 917,811 + LU632_RS04685 0.24 -0.9 917,938 - LU632_RS04685 0.58 +1.5 917,938 - LU632_RS04685 0.58 -1.2 917,985 + LU632_RS04685 0.71 +0.2 917,985 + LU632_RS04685 0.71 +0.5 917,985 + LU632_RS04685 0.71 -0.6 917,985 + LU632_RS04685 0.71 -0.4 917,986 - LU632_RS04685 0.72 -0.4 917,986 - LU632_RS04685 0.72 +0.6 917,992 - LU632_RS04685 0.73 +0.2 918,120 + -0.6 918,207 + LU632_RS04690 0.14 -0.8 918,231 + LU632_RS04690 0.17 +0.4 918,353 + LU632_RS04690 0.33 -1.0 918,354 - LU632_RS04690 0.33 -0.7 918,508 + LU632_RS04690 0.53 -0.4 918,508 + LU632_RS04690 0.53 -0.0 918,508 + LU632_RS04690 0.53 -3.2 918,508 + LU632_RS04690 0.53 +3.0 918,509 - LU632_RS04690 0.53 -0.6 918,509 - LU632_RS04690 0.53 -0.5 918,640 - LU632_RS04690 0.69 +0.2
Or see this region's nucleotide sequence