Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS04265 and ulaD overlap by 4 nucleotides ulaD and LU632_RS04275 are separated by 2 nucleotides LU632_RS04275 and araD overlap by 4 nucleotides araD and LU632_RS04285 are separated by 89 nucleotides
LU632_RS04265: LU632_RS04265 - carbohydrate kinase, at 848,197 to 849,681
_RS04265
LU632_RS04270: ulaD - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD, at 849,678 to 850,340
ulaD
LU632_RS04275: LU632_RS04275 - L-ribulose-5-phosphate 3-epimerase, at 850,343 to 851,200
_RS04275
LU632_RS04280: araD - L-ribulose-5-phosphate 4-epimerase, at 851,197 to 851,895
araD
LU632_RS04285: LU632_RS04285 - IS5 family transposase, at 851,985 to 852,801
_RS04285
Position (kb) 850
851
852 Strain fitness (log2 ratio) -2
-1
0
1 at 849.379 kb on - strand, within LU632_RS04265 at 849.673 kb on - strand at 849.876 kb on - strand, within ulaD at 850.090 kb on - strand, within ulaD at 850.235 kb on - strand, within ulaD at 850.241 kb on - strand, within ulaD at 850.241 kb on - strand, within ulaD at 850.241 kb on - strand, within ulaD at 850.268 kb on + strand, within ulaD at 850.325 kb on + strand at 850.337 kb on - strand at 850.338 kb on + strand at 850.338 kb on + strand at 850.338 kb on + strand at 850.339 kb on - strand at 850.339 kb on - strand at 850.339 kb on - strand at 850.339 kb on - strand at 850.365 kb on + strand at 850.365 kb on + strand at 850.366 kb on - strand at 850.366 kb on - strand at 850.368 kb on - strand at 850.368 kb on - strand at 850.368 kb on - strand at 850.455 kb on + strand, within LU632_RS04275 at 850.548 kb on + strand, within LU632_RS04275 at 850.592 kb on - strand, within LU632_RS04275 at 850.622 kb on + strand, within LU632_RS04275 at 850.701 kb on - strand, within LU632_RS04275 at 850.701 kb on - strand, within LU632_RS04275 at 850.703 kb on + strand, within LU632_RS04275 at 850.819 kb on + strand, within LU632_RS04275 at 850.834 kb on + strand, within LU632_RS04275 at 850.834 kb on + strand, within LU632_RS04275 at 850.881 kb on + strand, within LU632_RS04275 at 850.882 kb on - strand, within LU632_RS04275 at 850.882 kb on - strand, within LU632_RS04275 at 851.256 kb on + strand at 851.256 kb on + strand at 851.257 kb on - strand at 851.257 kb on - strand at 851.341 kb on + strand, within araD at 851.364 kb on + strand, within araD at 851.364 kb on + strand, within araD at 851.365 kb on - strand, within araD at 851.365 kb on - strand, within araD at 851.399 kb on + strand, within araD at 851.485 kb on - strand, within araD at 851.485 kb on - strand, within araD at 851.621 kb on - strand, within araD at 851.626 kb on + strand, within araD at 851.627 kb on - strand, within araD at 851.627 kb on - strand, within araD at 851.647 kb on - strand, within araD at 851.648 kb on + strand, within araD at 851.649 kb on - strand, within araD at 851.649 kb on - strand, within araD at 851.670 kb on + strand, within araD at 851.730 kb on + strand, within araD at 851.762 kb on + strand, within araD at 851.763 kb on - strand, within araD at 851.782 kb on + strand, within araD at 851.782 kb on + strand, within araD at 851.782 kb on + strand, within araD at 851.783 kb on - strand, within araD at 851.783 kb on - strand, within araD at 851.783 kb on - strand, within araD at 851.783 kb on - strand, within araD at 851.783 kb on - strand, within araD at 851.855 kb on - strand at 851.882 kb on - strand at 851.896 kb on + strand at 851.899 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 849,379 - LU632_RS04265 0.80 +1.3 849,673 - +0.3 849,876 - ulaD LU632_RS04270 0.30 +0.2 850,090 - ulaD LU632_RS04270 0.62 -0.1 850,235 - ulaD LU632_RS04270 0.84 +0.3 850,241 - ulaD LU632_RS04270 0.85 +0.1 850,241 - ulaD LU632_RS04270 0.85 +0.3 850,241 - ulaD LU632_RS04270 0.85 +1.3 850,268 + ulaD LU632_RS04270 0.89 -0.3 850,325 + +0.1 850,337 - +0.2 850,338 + +0.4 850,338 + +0.2 850,338 + +0.4 850,339 - -1.1 850,339 - +1.5 850,339 - -0.1 850,339 - +0.1 850,365 + -1.3 850,365 + +0.4 850,366 - +0.6 850,366 - -0.8 850,368 - -0.2 850,368 - -0.1 850,368 - -0.1 850,455 + LU632_RS04275 0.13 +0.9 850,548 + LU632_RS04275 0.24 -1.4 850,592 - LU632_RS04275 0.29 -0.2 850,622 + LU632_RS04275 0.33 -0.2 850,701 - LU632_RS04275 0.42 -0.4 850,701 - LU632_RS04275 0.42 -0.5 850,703 + LU632_RS04275 0.42 -0.1 850,819 + LU632_RS04275 0.55 +0.4 850,834 + LU632_RS04275 0.57 -0.1 850,834 + LU632_RS04275 0.57 +1.2 850,881 + LU632_RS04275 0.63 -0.1 850,882 - LU632_RS04275 0.63 +0.2 850,882 - LU632_RS04275 0.63 +0.3 851,256 + +0.3 851,256 + +0.1 851,257 - +0.7 851,257 - -0.2 851,341 + araD LU632_RS04280 0.21 -1.9 851,364 + araD LU632_RS04280 0.24 -0.1 851,364 + araD LU632_RS04280 0.24 +0.7 851,365 - araD LU632_RS04280 0.24 +0.4 851,365 - araD LU632_RS04280 0.24 +0.8 851,399 + araD LU632_RS04280 0.29 +0.5 851,485 - araD LU632_RS04280 0.41 +1.3 851,485 - araD LU632_RS04280 0.41 -0.6 851,621 - araD LU632_RS04280 0.61 +1.1 851,626 + araD LU632_RS04280 0.61 -0.4 851,627 - araD LU632_RS04280 0.62 +0.7 851,627 - araD LU632_RS04280 0.62 +0.8 851,647 - araD LU632_RS04280 0.64 +0.6 851,648 + araD LU632_RS04280 0.65 -0.2 851,649 - araD LU632_RS04280 0.65 +0.2 851,649 - araD LU632_RS04280 0.65 +0.4 851,670 + araD LU632_RS04280 0.68 +0.1 851,730 + araD LU632_RS04280 0.76 +0.4 851,762 + araD LU632_RS04280 0.81 -0.3 851,763 - araD LU632_RS04280 0.81 +0.1 851,782 + araD LU632_RS04280 0.84 -0.1 851,782 + araD LU632_RS04280 0.84 +0.8 851,782 + araD LU632_RS04280 0.84 -1.3 851,783 - araD LU632_RS04280 0.84 -0.1 851,783 - araD LU632_RS04280 0.84 -0.8 851,783 - araD LU632_RS04280 0.84 +0.0 851,783 - araD LU632_RS04280 0.84 -0.1 851,783 - araD LU632_RS04280 0.84 -0.4 851,855 - -1.6 851,882 - -1.2 851,896 + -0.1 851,899 + +0.9
Or see this region's nucleotide sequence