Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS01330 and LU632_RS01335 are separated by 6 nucleotides LU632_RS01335 and LU632_RS01340 are separated by 570 nucleotides LU632_RS01340 and LU632_RS01345 are separated by 586 nucleotides LU632_RS01345 and LU632_RS01350 are separated by 133 nucleotides
LU632_RS01330: LU632_RS01330 - sugar porter family MFS transporter, at 274,655 to 274,951
_RS01330
LU632_RS01335: LU632_RS01335 - MFS transporter, at 274,958 to 275,260
_RS01335
LU632_RS01340: LU632_RS01340 - hypothetical protein, at 275,831 to 282,493
_RS01340
LU632_RS01345: LU632_RS01345 - transposase, at 283,080 to 283,229
_RS01345
LU632_RS01350: LU632_RS01350 - lactonase family protein, at 283,363 to 284,469
_RS01350
Position (kb)
275
276
277
278
279
280
281
282
283 Strain fitness (log2 ratio)
-1
0
1 at 274.882 kb on + strand, within LU632_RS01330 at 274.883 kb on - strand, within LU632_RS01330 at 274.883 kb on - strand, within LU632_RS01330 at 274.883 kb on - strand, within LU632_RS01330 at 274.883 kb on - strand, within LU632_RS01330 at 274.883 kb on - strand, within LU632_RS01330 at 274.969 kb on + strand at 274.983 kb on - strand at 275.095 kb on + strand, within LU632_RS01335 at 275.095 kb on + strand, within LU632_RS01335 at 275.096 kb on - strand, within LU632_RS01335 at 275.159 kb on + strand, within LU632_RS01335 at 275.176 kb on - strand, within LU632_RS01335 at 275.176 kb on - strand, within LU632_RS01335 at 275.176 kb on - strand, within LU632_RS01335 at 275.221 kb on + strand, within LU632_RS01335 at 275.299 kb on + strand at 275.300 kb on - strand at 275.303 kb on + strand at 275.303 kb on + strand at 275.303 kb on + strand at 275.303 kb on + strand at 275.304 kb on - strand at 275.304 kb on - strand at 275.304 kb on - strand at 275.304 kb on - strand at 275.304 kb on - strand at 275.332 kb on - strand at 275.332 kb on - strand at 275.332 kb on - strand at 275.332 kb on - strand at 275.332 kb on - strand at 275.332 kb on - strand at 275.333 kb on + strand at 275.333 kb on + strand at 275.334 kb on - strand at 275.334 kb on - strand at 275.334 kb on - strand at 275.772 kb on - strand at 276.971 kb on - strand, within LU632_RS01340 at 276.971 kb on - strand, within LU632_RS01340 at 282.992 kb on + strand at 282.993 kb on - strand at 282.996 kb on - strand at 283.001 kb on + strand at 283.214 kb on + strand, within LU632_RS01345 at 283.221 kb on + strand at 283.221 kb on + strand at 283.229 kb on + strand at 283.229 kb on + strand at 283.258 kb on + strand at 283.259 kb on - strand at 283.454 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 274,882 + LU632_RS01330 0.76 -0.4 274,883 - LU632_RS01330 0.77 -0.0 274,883 - LU632_RS01330 0.77 +0.3 274,883 - LU632_RS01330 0.77 -0.1 274,883 - LU632_RS01330 0.77 +0.0 274,883 - LU632_RS01330 0.77 +0.0 274,969 + -0.1 274,983 - -0.3 275,095 + LU632_RS01335 0.45 +1.5 275,095 + LU632_RS01335 0.45 +0.0 275,096 - LU632_RS01335 0.46 -0.2 275,159 + LU632_RS01335 0.66 -0.4 275,176 - LU632_RS01335 0.72 -0.1 275,176 - LU632_RS01335 0.72 +0.1 275,176 - LU632_RS01335 0.72 +0.9 275,221 + LU632_RS01335 0.87 -0.5 275,299 + -0.2 275,300 - +0.2 275,303 + +0.1 275,303 + -0.6 275,303 + -0.1 275,303 + -0.3 275,304 - -1.5 275,304 - -0.3 275,304 - -1.2 275,304 - +0.4 275,304 - -0.6 275,332 - -0.1 275,332 - +0.6 275,332 - -1.1 275,332 - -0.9 275,332 - +0.3 275,332 - -0.1 275,333 + -0.4 275,333 + -0.1 275,334 - -0.2 275,334 - +0.1 275,334 - +0.2 275,772 - +0.5 276,971 - LU632_RS01340 0.17 -0.5 276,971 - LU632_RS01340 0.17 -0.3 282,992 + +0.9 282,993 - +0.0 282,996 - -0.6 283,001 + -0.4 283,214 + LU632_RS01345 0.89 -0.5 283,221 + -0.5 283,221 + -0.3 283,229 + -0.6 283,229 + +0.0 283,258 + +0.0 283,259 - +0.6 283,454 + +0.0
Or see this region's nucleotide sequence