Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpcA and LU632_RS01140 are separated by 43 nucleotides LU632_RS01140 and dpaA overlap by 30 nucleotides dpaA and dinB are separated by 235 nucleotides
LU632_RS01135: lpcA - D-sedoheptulose 7-phosphate isomerase, at 241,854 to 242,432
lpcA
LU632_RS01140: LU632_RS01140 - class II glutamine amidotransferase, at 242,476 to 243,243
_RS01140
LU632_RS01145: dpaA - peptidoglycan meso-diaminopimelic acid protein amidase, at 243,214 to 243,957
dpaA
LU632_RS01150: dinB - DNA polymerase IV, at 244,193 to 245,248
dinB
Position (kb) 243
244 Strain fitness (log2 ratio) -2
-1
0
1 at 242.379 kb on - strand at 242.430 kb on + strand at 242.469 kb on + strand at 242.469 kb on + strand at 242.470 kb on - strand at 242.470 kb on - strand at 242.470 kb on - strand at 242.476 kb on - strand at 242.622 kb on + strand, within LU632_RS01140 at 242.623 kb on - strand, within LU632_RS01140 at 242.623 kb on - strand, within LU632_RS01140 at 242.623 kb on - strand, within LU632_RS01140 at 242.623 kb on - strand, within LU632_RS01140 at 242.719 kb on - strand, within LU632_RS01140 at 242.745 kb on + strand, within LU632_RS01140 at 242.768 kb on + strand, within LU632_RS01140 at 242.769 kb on - strand, within LU632_RS01140 at 242.778 kb on + strand, within LU632_RS01140 at 242.788 kb on + strand, within LU632_RS01140 at 242.788 kb on + strand, within LU632_RS01140 at 242.789 kb on - strand, within LU632_RS01140 at 242.808 kb on - strand, within LU632_RS01140 at 242.808 kb on - strand, within LU632_RS01140 at 242.994 kb on + strand, within LU632_RS01140 at 242.994 kb on + strand, within LU632_RS01140 at 242.994 kb on + strand, within LU632_RS01140 at 242.995 kb on - strand, within LU632_RS01140 at 242.995 kb on - strand, within LU632_RS01140 at 243.022 kb on - strand, within LU632_RS01140 at 243.022 kb on - strand, within LU632_RS01140 at 243.274 kb on - strand at 243.290 kb on - strand, within dpaA at 243.294 kb on - strand, within dpaA at 243.492 kb on - strand, within dpaA at 243.492 kb on - strand, within dpaA at 243.495 kb on + strand, within dpaA at 243.495 kb on + strand, within dpaA at 243.496 kb on - strand, within dpaA at 243.547 kb on - strand, within dpaA at 243.564 kb on - strand, within dpaA at 243.601 kb on - strand, within dpaA at 243.907 kb on + strand at 243.975 kb on + strand at 243.975 kb on + strand at 243.987 kb on + strand at 243.988 kb on - strand at 243.988 kb on - strand at 243.988 kb on - strand at 244.034 kb on - strand at 244.051 kb on - strand at 244.198 kb on + strand at 244.210 kb on + strand at 244.210 kb on + strand at 244.324 kb on + strand, within dinB at 244.352 kb on + strand, within dinB at 244.353 kb on - strand, within dinB at 244.427 kb on + strand, within dinB at 244.428 kb on - strand, within dinB at 244.429 kb on + strand, within dinB at 244.430 kb on - strand, within dinB at 244.448 kb on - strand, within dinB at 244.508 kb on + strand, within dinB at 244.508 kb on + strand, within dinB at 244.509 kb on - strand, within dinB at 244.518 kb on + strand, within dinB at 244.518 kb on + strand, within dinB at 244.519 kb on - strand, within dinB at 244.519 kb on - strand, within dinB at 244.539 kb on - strand, within dinB at 244.580 kb on - strand, within dinB at 244.948 kb on + strand, within dinB
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 242,379 - -1.1 242,430 + -0.3 242,469 + +0.9 242,469 + -2.4 242,470 - +0.2 242,470 - +0.1 242,470 - +1.5 242,476 - +1.2 242,622 + LU632_RS01140 0.19 +0.4 242,623 - LU632_RS01140 0.19 +1.3 242,623 - LU632_RS01140 0.19 -0.2 242,623 - LU632_RS01140 0.19 +0.3 242,623 - LU632_RS01140 0.19 +0.9 242,719 - LU632_RS01140 0.32 -1.4 242,745 + LU632_RS01140 0.35 +0.3 242,768 + LU632_RS01140 0.38 +1.1 242,769 - LU632_RS01140 0.38 -1.4 242,778 + LU632_RS01140 0.39 -1.3 242,788 + LU632_RS01140 0.41 +0.1 242,788 + LU632_RS01140 0.41 -0.4 242,789 - LU632_RS01140 0.41 -0.5 242,808 - LU632_RS01140 0.43 -0.4 242,808 - LU632_RS01140 0.43 +0.0 242,994 + LU632_RS01140 0.67 -0.1 242,994 + LU632_RS01140 0.67 +0.3 242,994 + LU632_RS01140 0.67 -0.6 242,995 - LU632_RS01140 0.68 +0.5 242,995 - LU632_RS01140 0.68 +0.4 243,022 - LU632_RS01140 0.71 -0.5 243,022 - LU632_RS01140 0.71 -0.8 243,274 - -0.4 243,290 - dpaA LU632_RS01145 0.10 -0.2 243,294 - dpaA LU632_RS01145 0.11 +0.4 243,492 - dpaA LU632_RS01145 0.37 +0.0 243,492 - dpaA LU632_RS01145 0.37 +0.1 243,495 + dpaA LU632_RS01145 0.38 +1.2 243,495 + dpaA LU632_RS01145 0.38 +0.6 243,496 - dpaA LU632_RS01145 0.38 -0.9 243,547 - dpaA LU632_RS01145 0.45 -0.2 243,564 - dpaA LU632_RS01145 0.47 -0.9 243,601 - dpaA LU632_RS01145 0.52 -0.6 243,907 + +0.1 243,975 + -0.1 243,975 + -0.2 243,987 + -1.7 243,988 - -0.1 243,988 - -0.1 243,988 - -0.3 244,034 - +0.4 244,051 - +0.5 244,198 + -0.4 244,210 + -0.1 244,210 + -0.1 244,324 + dinB LU632_RS01150 0.12 +0.2 244,352 + dinB LU632_RS01150 0.15 -0.0 244,353 - dinB LU632_RS01150 0.15 -0.3 244,427 + dinB LU632_RS01150 0.22 +0.1 244,428 - dinB LU632_RS01150 0.22 -0.2 244,429 + dinB LU632_RS01150 0.22 +0.1 244,430 - dinB LU632_RS01150 0.22 +0.9 244,448 - dinB LU632_RS01150 0.24 +0.3 244,508 + dinB LU632_RS01150 0.30 +0.3 244,508 + dinB LU632_RS01150 0.30 +0.1 244,509 - dinB LU632_RS01150 0.30 +1.0 244,518 + dinB LU632_RS01150 0.31 +0.3 244,518 + dinB LU632_RS01150 0.31 -0.9 244,519 - dinB LU632_RS01150 0.31 -0.0 244,519 - dinB LU632_RS01150 0.31 +0.1 244,539 - dinB LU632_RS01150 0.33 +0.1 244,580 - dinB LU632_RS01150 0.37 -0.5 244,948 + dinB LU632_RS01150 0.71 +0.5
Or see this region's nucleotide sequence