Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt polA and dsbA are separated by 400 nucleotides dsbA and LU632_RS23880 are separated by 48 nucleotides
LU632_RS23870: polA - DNA polymerase I, at 4,550,499 to 4,553,282
polA
LU632_RS23875: dsbA - thiol:disulfide interchange protein DsbA, at 4,553,683 to 4,554,312
dsbA
LU632_RS23880: LU632_RS23880 - serine/threonine protein kinase, at 4,554,361 to 4,555,353
_RS23880
Position (kb)
4553
4554
4555 Strain fitness (log2 ratio)
-2
-1
0
1 at 4553.354 kb on - strand at 4553.441 kb on - strand at 4553.455 kb on + strand at 4553.639 kb on - strand at 4553.732 kb on + strand at 4553.732 kb on + strand at 4553.733 kb on - strand at 4553.777 kb on + strand, within dsbA at 4553.777 kb on + strand, within dsbA at 4553.889 kb on - strand, within dsbA at 4554.339 kb on - strand at 4554.350 kb on - strand at 4554.525 kb on - strand, within LU632_RS23880 at 4554.527 kb on + strand, within LU632_RS23880 at 4554.528 kb on - strand, within LU632_RS23880 at 4554.540 kb on + strand, within LU632_RS23880 at 4554.540 kb on + strand, within LU632_RS23880 at 4554.547 kb on + strand, within LU632_RS23880 at 4554.574 kb on + strand, within LU632_RS23880 at 4554.574 kb on + strand, within LU632_RS23880 at 4554.575 kb on - strand, within LU632_RS23880 at 4554.590 kb on + strand, within LU632_RS23880 at 4554.599 kb on + strand, within LU632_RS23880 at 4554.610 kb on + strand, within LU632_RS23880 at 4554.611 kb on - strand, within LU632_RS23880 at 4554.611 kb on - strand, within LU632_RS23880 at 4554.611 kb on - strand, within LU632_RS23880 at 4554.613 kb on + strand, within LU632_RS23880 at 4554.614 kb on - strand, within LU632_RS23880 at 4554.628 kb on - strand, within LU632_RS23880 at 4554.628 kb on - strand, within LU632_RS23880 at 4554.767 kb on - strand, within LU632_RS23880 at 4554.799 kb on + strand, within LU632_RS23880 at 4554.800 kb on - strand, within LU632_RS23880 at 4554.813 kb on + strand, within LU632_RS23880 at 4554.813 kb on + strand, within LU632_RS23880 at 4554.813 kb on + strand, within LU632_RS23880 at 4554.814 kb on - strand, within LU632_RS23880 at 4554.860 kb on - strand, within LU632_RS23880 at 4554.899 kb on + strand, within LU632_RS23880 at 4554.900 kb on - strand, within LU632_RS23880 at 4554.979 kb on + strand, within LU632_RS23880 at 4554.998 kb on + strand, within LU632_RS23880 at 4555.003 kb on - strand, within LU632_RS23880 at 4555.014 kb on - strand, within LU632_RS23880 at 4555.200 kb on - strand, within LU632_RS23880 at 4555.245 kb on - strand, within LU632_RS23880
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 4,553,354 - +0.1 4,553,441 - -2.2 4,553,455 + -0.2 4,553,639 - -0.2 4,553,732 + -1.1 4,553,732 + -0.8 4,553,733 - -0.7 4,553,777 + dsbA LU632_RS23875 0.15 -0.4 4,553,777 + dsbA LU632_RS23875 0.15 -1.8 4,553,889 - dsbA LU632_RS23875 0.33 +0.9 4,554,339 - +1.7 4,554,350 - +0.8 4,554,525 - LU632_RS23880 0.17 +0.0 4,554,527 + LU632_RS23880 0.17 +0.3 4,554,528 - LU632_RS23880 0.17 +0.0 4,554,540 + LU632_RS23880 0.18 +1.2 4,554,540 + LU632_RS23880 0.18 -0.7 4,554,547 + LU632_RS23880 0.19 -0.4 4,554,574 + LU632_RS23880 0.21 -0.1 4,554,574 + LU632_RS23880 0.21 -0.1 4,554,575 - LU632_RS23880 0.22 -0.3 4,554,590 + LU632_RS23880 0.23 -1.0 4,554,599 + LU632_RS23880 0.24 +0.4 4,554,610 + LU632_RS23880 0.25 -0.2 4,554,611 - LU632_RS23880 0.25 +0.2 4,554,611 - LU632_RS23880 0.25 +1.3 4,554,611 - LU632_RS23880 0.25 +0.4 4,554,613 + LU632_RS23880 0.25 -1.4 4,554,614 - LU632_RS23880 0.25 +1.0 4,554,628 - LU632_RS23880 0.27 -0.4 4,554,628 - LU632_RS23880 0.27 +0.1 4,554,767 - LU632_RS23880 0.41 -0.6 4,554,799 + LU632_RS23880 0.44 -0.7 4,554,800 - LU632_RS23880 0.44 +0.8 4,554,813 + LU632_RS23880 0.46 -0.2 4,554,813 + LU632_RS23880 0.46 -0.0 4,554,813 + LU632_RS23880 0.46 -0.2 4,554,814 - LU632_RS23880 0.46 +0.2 4,554,860 - LU632_RS23880 0.50 -0.7 4,554,899 + LU632_RS23880 0.54 +0.1 4,554,900 - LU632_RS23880 0.54 +0.4 4,554,979 + LU632_RS23880 0.62 -0.0 4,554,998 + LU632_RS23880 0.64 +0.1 4,555,003 - LU632_RS23880 0.65 +0.0 4,555,014 - LU632_RS23880 0.66 +1.1 4,555,200 - LU632_RS23880 0.84 -0.8 4,555,245 - LU632_RS23880 0.89 +0.5
Or see this region's nucleotide sequence