Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tusB and tusC are separated by 6 nucleotides tusC and tusD overlap by 1 nucleotides tusD and LU632_RS22710 overlap by 1 nucleotides LU632_RS22710 and fkpA are separated by 196 nucleotides
LU632_RS22695: tusB - sulfurtransferase complex subunit TusB, at 4,322,674 to 4,322,961
tusB
LU632_RS22700: tusC - sulfurtransferase complex subunit TusC, at 4,322,968 to 4,323,327
tusC
LU632_RS22705: tusD - sulfurtransferase complex subunit TusD, at 4,323,327 to 4,323,713
tusD
LU632_RS22710: LU632_RS22710 - transcriptional regulator, at 4,323,713 to 4,324,435
_RS22710
LU632_RS22715: fkpA - FKBP-type peptidyl-prolyl cis-trans isomerase, at 4,324,632 to 4,325,471
fkpA
Position (kb)
4323
4324
4325 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4324.109 kb on - strand, within LU632_RS22710 at 4324.138 kb on - strand, within LU632_RS22710 at 4324.138 kb on - strand, within LU632_RS22710 at 4324.138 kb on - strand, within LU632_RS22710 at 4324.154 kb on - strand, within LU632_RS22710 at 4324.229 kb on - strand, within LU632_RS22710 at 4324.342 kb on - strand, within LU632_RS22710 at 4324.342 kb on - strand, within LU632_RS22710 at 4324.422 kb on - strand at 4324.624 kb on + strand at 4324.624 kb on + strand at 4324.634 kb on - strand at 4324.634 kb on - strand at 4324.802 kb on + strand, within fkpA at 4324.901 kb on + strand, within fkpA at 4324.934 kb on + strand, within fkpA at 4324.936 kb on + strand, within fkpA at 4324.940 kb on + strand, within fkpA at 4324.941 kb on - strand, within fkpA at 4324.944 kb on + strand, within fkpA at 4324.945 kb on - strand, within fkpA at 4325.272 kb on + strand, within fkpA at 4325.272 kb on + strand, within fkpA
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 4,324,109 - LU632_RS22710 0.55 +0.3 4,324,138 - LU632_RS22710 0.59 -0.7 4,324,138 - LU632_RS22710 0.59 -0.3 4,324,138 - LU632_RS22710 0.59 -1.1 4,324,154 - LU632_RS22710 0.61 -0.1 4,324,229 - LU632_RS22710 0.71 +0.9 4,324,342 - LU632_RS22710 0.87 -1.2 4,324,342 - LU632_RS22710 0.87 -1.7 4,324,422 - -2.3 4,324,624 + -0.0 4,324,624 + -0.8 4,324,634 - -0.1 4,324,634 - +0.3 4,324,802 + fkpA LU632_RS22715 0.20 -0.8 4,324,901 + fkpA LU632_RS22715 0.32 +1.3 4,324,934 + fkpA LU632_RS22715 0.36 -0.6 4,324,936 + fkpA LU632_RS22715 0.36 +0.7 4,324,940 + fkpA LU632_RS22715 0.37 -0.4 4,324,941 - fkpA LU632_RS22715 0.37 -0.4 4,324,944 + fkpA LU632_RS22715 0.37 -1.0 4,324,945 - fkpA LU632_RS22715 0.37 -0.9 4,325,272 + fkpA LU632_RS22715 0.76 -0.7 4,325,272 + fkpA LU632_RS22715 0.76 +1.9
Or see this region's nucleotide sequence