Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dusB and fis overlap by 19 nucleotides fis and LU632_RS21520 are separated by 158 nucleotides
LU632_RS21510: dusB - tRNA dihydrouridine synthase DusB, at 4,115,016 to 4,116,024
dusB
LU632_RS21515: fis - DNA-binding transcriptional regulator Fis, at 4,116,006 to 4,116,302
fis
LU632_RS21520: LU632_RS21520 - IS91 family transposase, at 4,116,461 to 4,117,684
_RS21520
Position (kb)
4116
4117 Strain fitness (log2 ratio)
-1
0
1 at 4115.023 kb on + strand at 4115.120 kb on + strand, within dusB at 4115.120 kb on + strand, within dusB at 4115.121 kb on - strand, within dusB at 4115.270 kb on + strand, within dusB at 4115.271 kb on - strand, within dusB at 4115.322 kb on - strand, within dusB at 4115.337 kb on - strand, within dusB at 4115.452 kb on + strand, within dusB at 4115.452 kb on + strand, within dusB at 4115.452 kb on + strand, within dusB at 4115.452 kb on + strand, within dusB at 4115.452 kb on + strand, within dusB at 4115.452 kb on + strand, within dusB at 4115.452 kb on + strand, within dusB at 4115.453 kb on - strand, within dusB at 4115.462 kb on + strand, within dusB at 4115.470 kb on + strand, within dusB at 4115.475 kb on - strand, within dusB at 4115.528 kb on + strand, within dusB at 4115.529 kb on - strand, within dusB at 4115.659 kb on - strand, within dusB at 4115.780 kb on + strand, within dusB at 4115.780 kb on + strand, within dusB at 4115.800 kb on - strand, within dusB at 4115.803 kb on + strand, within dusB at 4115.823 kb on + strand, within dusB at 4115.872 kb on - strand, within dusB at 4115.961 kb on + strand at 4115.961 kb on + strand at 4115.962 kb on - strand at 4116.006 kb on - strand at 4116.006 kb on - strand at 4116.006 kb on - strand at 4116.022 kb on + strand at 4116.035 kb on - strand at 4116.054 kb on - strand, within fis at 4116.117 kb on + strand, within fis at 4116.131 kb on - strand, within fis at 4116.131 kb on - strand, within fis at 4116.156 kb on + strand, within fis at 4116.157 kb on - strand, within fis at 4116.157 kb on - strand, within fis at 4116.157 kb on - strand, within fis at 4116.197 kb on + strand, within fis at 4116.210 kb on + strand, within fis at 4116.212 kb on + strand, within fis at 4116.212 kb on + strand, within fis at 4116.212 kb on + strand, within fis
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 4,115,023 + +0.9 4,115,120 + dusB LU632_RS21510 0.10 -1.0 4,115,120 + dusB LU632_RS21510 0.10 -0.2 4,115,121 - dusB LU632_RS21510 0.10 -0.4 4,115,270 + dusB LU632_RS21510 0.25 -0.4 4,115,271 - dusB LU632_RS21510 0.25 -0.7 4,115,322 - dusB LU632_RS21510 0.30 +0.9 4,115,337 - dusB LU632_RS21510 0.32 -0.2 4,115,452 + dusB LU632_RS21510 0.43 -0.6 4,115,452 + dusB LU632_RS21510 0.43 -0.4 4,115,452 + dusB LU632_RS21510 0.43 +0.3 4,115,452 + dusB LU632_RS21510 0.43 -0.3 4,115,452 + dusB LU632_RS21510 0.43 -0.3 4,115,452 + dusB LU632_RS21510 0.43 -0.4 4,115,452 + dusB LU632_RS21510 0.43 -0.7 4,115,453 - dusB LU632_RS21510 0.43 -0.2 4,115,462 + dusB LU632_RS21510 0.44 +0.6 4,115,470 + dusB LU632_RS21510 0.45 -1.7 4,115,475 - dusB LU632_RS21510 0.45 -1.1 4,115,528 + dusB LU632_RS21510 0.51 -0.1 4,115,529 - dusB LU632_RS21510 0.51 -1.1 4,115,659 - dusB LU632_RS21510 0.64 +0.1 4,115,780 + dusB LU632_RS21510 0.76 +0.9 4,115,780 + dusB LU632_RS21510 0.76 -1.0 4,115,800 - dusB LU632_RS21510 0.78 -0.1 4,115,803 + dusB LU632_RS21510 0.78 -0.7 4,115,823 + dusB LU632_RS21510 0.80 -1.7 4,115,872 - dusB LU632_RS21510 0.85 -0.0 4,115,961 + +0.4 4,115,961 + +0.7 4,115,962 - -0.4 4,116,006 - -1.7 4,116,006 - -0.4 4,116,006 - -0.4 4,116,022 + -1.7 4,116,035 - +0.2 4,116,054 - fis LU632_RS21515 0.16 -1.1 4,116,117 + fis LU632_RS21515 0.37 -0.4 4,116,131 - fis LU632_RS21515 0.42 -0.4 4,116,131 - fis LU632_RS21515 0.42 -0.5 4,116,156 + fis LU632_RS21515 0.51 -0.0 4,116,157 - fis LU632_RS21515 0.51 -1.2 4,116,157 - fis LU632_RS21515 0.51 +0.1 4,116,157 - fis LU632_RS21515 0.51 -1.0 4,116,197 + fis LU632_RS21515 0.64 -0.1 4,116,210 + fis LU632_RS21515 0.69 -1.0 4,116,212 + fis LU632_RS21515 0.69 -0.7 4,116,212 + fis LU632_RS21515 0.69 -0.7 4,116,212 + fis LU632_RS21515 0.69 -0.2
Or see this region's nucleotide sequence