Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdsC and lptC overlap by 4 nucleotides lptC and lptA overlap by 29 nucleotides lptA and lptB are separated by 10 nucleotides lptB and rpoN are separated by 46 nucleotides
LU632_RS21260: kdsC - 3-deoxy-manno-octulosonate-8-phosphatase KdsC, at 4,059,708 to 4,060,274
kdsC
LU632_RS21265: lptC - LPS export ABC transporter periplasmic protein LptC, at 4,060,271 to 4,060,846
lptC
LU632_RS21270: lptA - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 4,060,818 to 4,061,366
lptA
LU632_RS21275: lptB - LPS export ABC transporter ATP-binding protein, at 4,061,377 to 4,062,102
lptB
LU632_RS21280: rpoN - RNA polymerase factor sigma-54, at 4,062,149 to 4,063,582
rpoN
Position (kb) 4060
4061
4062 Strain fitness (log2 ratio) -2
-1
0
1 at 4060.071 kb on + strand, within kdsC at 4060.071 kb on + strand, within kdsC at 4060.073 kb on + strand, within kdsC at 4060.073 kb on + strand, within kdsC at 4060.073 kb on + strand, within kdsC at 4060.073 kb on + strand, within kdsC at 4060.073 kb on + strand, within kdsC at 4060.073 kb on + strand, within kdsC at 4060.073 kb on + strand, within kdsC at 4060.166 kb on + strand, within kdsC at 4060.170 kb on + strand, within kdsC at 4060.170 kb on + strand, within kdsC at 4060.170 kb on + strand, within kdsC at 4061.364 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 4,060,071 + kdsC LU632_RS21260 0.64 +0.0 4,060,071 + kdsC LU632_RS21260 0.64 +0.9 4,060,073 + kdsC LU632_RS21260 0.64 +0.1 4,060,073 + kdsC LU632_RS21260 0.64 -2.0 4,060,073 + kdsC LU632_RS21260 0.64 +1.2 4,060,073 + kdsC LU632_RS21260 0.64 +0.3 4,060,073 + kdsC LU632_RS21260 0.64 -0.8 4,060,073 + kdsC LU632_RS21260 0.64 +0.0 4,060,073 + kdsC LU632_RS21260 0.64 -1.1 4,060,166 + kdsC LU632_RS21260 0.81 +0.7 4,060,170 + kdsC LU632_RS21260 0.81 -1.1 4,060,170 + kdsC LU632_RS21260 0.81 -0.4 4,060,170 + kdsC LU632_RS21260 0.81 +0.3 4,061,364 + +0.5
Or see this region's nucleotide sequence