Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS20310 and LU632_RS20315 overlap by 27 nucleotides LU632_RS20315 and LU632_RS20320 are separated by 70 nucleotides LU632_RS20320 and LU632_RS20325 overlap by 8 nucleotides LU632_RS20325 and LU632_RS20330 are separated by 119 nucleotides
LU632_RS20310: LU632_RS20310 - helix-turn-helix transcriptional regulator, at 3,865,529 to 3,866,032
_RS20310
LU632_RS20315: LU632_RS20315 - hypothetical protein, at 3,866,006 to 3,866,338
_RS20315
LU632_RS20320: LU632_RS20320 - hypothetical protein, at 3,866,409 to 3,866,753
_RS20320
LU632_RS20325: LU632_RS20325 - hypothetical protein, at 3,866,746 to 3,866,940
_RS20325
LU632_RS20330: LU632_RS20330 - IS6 family transposase, at 3,867,060 to 3,867,768
_RS20330
Position (kb)
3866
3867 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3865.850 kb on - strand, within LU632_RS20310 at 3865.904 kb on - strand, within LU632_RS20310 at 3865.912 kb on + strand, within LU632_RS20310 at 3865.940 kb on + strand, within LU632_RS20310 at 3865.940 kb on + strand, within LU632_RS20310 at 3865.965 kb on + strand, within LU632_RS20310 at 3866.012 kb on + strand at 3866.127 kb on - strand, within LU632_RS20315 at 3866.136 kb on + strand, within LU632_RS20315 at 3866.136 kb on + strand, within LU632_RS20315 at 3866.137 kb on - strand, within LU632_RS20315 at 3866.184 kb on + strand, within LU632_RS20315 at 3866.184 kb on + strand, within LU632_RS20315 at 3866.185 kb on - strand, within LU632_RS20315 at 3866.185 kb on - strand, within LU632_RS20315 at 3866.313 kb on - strand at 3866.553 kb on + strand, within LU632_RS20320 at 3866.754 kb on - strand at 3866.754 kb on - strand at 3866.768 kb on + strand, within LU632_RS20325 at 3866.768 kb on + strand, within LU632_RS20325 at 3866.768 kb on + strand, within LU632_RS20325 at 3866.780 kb on + strand, within LU632_RS20325 at 3866.781 kb on - strand, within LU632_RS20325 at 3866.826 kb on + strand, within LU632_RS20325 at 3866.829 kb on - strand, within LU632_RS20325 at 3866.835 kb on + strand, within LU632_RS20325 at 3866.861 kb on + strand, within LU632_RS20325 at 3867.063 kb on + strand at 3867.090 kb on + strand at 3867.090 kb on + strand at 3867.090 kb on + strand at 3867.090 kb on + strand at 3867.090 kb on + strand at 3867.202 kb on + strand, within LU632_RS20330 at 3867.262 kb on + strand, within LU632_RS20330 at 3867.919 kb on + strand at 3867.920 kb on - strand at 3867.926 kb on - strand at 3867.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 3,865,850 - LU632_RS20310 0.64 -0.7 3,865,904 - LU632_RS20310 0.74 +0.3 3,865,912 + LU632_RS20310 0.76 +0.6 3,865,940 + LU632_RS20310 0.82 -0.3 3,865,940 + LU632_RS20310 0.82 -0.7 3,865,965 + LU632_RS20310 0.87 +0.3 3,866,012 + -1.9 3,866,127 - LU632_RS20315 0.36 -2.3 3,866,136 + LU632_RS20315 0.39 -0.5 3,866,136 + LU632_RS20315 0.39 -0.9 3,866,137 - LU632_RS20315 0.39 -1.4 3,866,184 + LU632_RS20315 0.53 -0.4 3,866,184 + LU632_RS20315 0.53 -0.3 3,866,185 - LU632_RS20315 0.54 +0.5 3,866,185 - LU632_RS20315 0.54 -0.7 3,866,313 - +0.4 3,866,553 + LU632_RS20320 0.42 +1.9 3,866,754 - -0.2 3,866,754 - -0.3 3,866,768 + LU632_RS20325 0.11 -3.0 3,866,768 + LU632_RS20325 0.11 -1.2 3,866,768 + LU632_RS20325 0.11 -1.0 3,866,780 + LU632_RS20325 0.17 -0.9 3,866,781 - LU632_RS20325 0.18 +0.0 3,866,826 + LU632_RS20325 0.41 -0.8 3,866,829 - LU632_RS20325 0.43 -0.9 3,866,835 + LU632_RS20325 0.46 -1.7 3,866,861 + LU632_RS20325 0.59 -1.1 3,867,063 + -0.6 3,867,090 + +0.6 3,867,090 + -0.7 3,867,090 + +0.9 3,867,090 + +0.1 3,867,090 + -1.6 3,867,202 + LU632_RS20330 0.20 +0.4 3,867,262 + LU632_RS20330 0.28 +0.3 3,867,919 + -0.4 3,867,920 - +0.3 3,867,926 - -0.3 3,867,938 - +0.7
Or see this region's nucleotide sequence