Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hrpB and thpR are separated by 77 nucleotides thpR and sfsA overlap by 4 nucleotides sfsA and dksA are separated by 170 nucleotides
LU632_RS20065: hrpB - ATP-dependent helicase HrpB, at 3,821,505 to 3,823,946
hrpB
LU632_RS20070: thpR - RNA 2',3'-cyclic phosphodiesterase, at 3,824,024 to 3,824,563
thpR
LU632_RS20075: sfsA - DNA/RNA nuclease SfsA, at 3,824,560 to 3,825,264
sfsA
LU632_RS20080: dksA - RNA polymerase-binding protein DksA, at 3,825,435 to 3,825,890
dksA
Position (kb)
3824
3825 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3823.107 kb on - strand, within hrpB at 3823.211 kb on - strand, within hrpB at 3823.623 kb on + strand, within hrpB at 3823.624 kb on - strand, within hrpB at 3823.640 kb on + strand, within hrpB at 3823.788 kb on + strand at 3823.872 kb on - strand at 3823.872 kb on - strand at 3823.953 kb on - strand at 3823.990 kb on + strand at 3823.990 kb on + strand at 3823.991 kb on - strand at 3824.083 kb on + strand, within thpR at 3824.083 kb on + strand, within thpR at 3824.083 kb on + strand, within thpR at 3824.203 kb on - strand, within thpR at 3824.398 kb on + strand, within thpR at 3824.399 kb on - strand, within thpR at 3824.466 kb on - strand, within thpR at 3824.485 kb on - strand, within thpR at 3824.519 kb on + strand at 3824.521 kb on + strand at 3824.521 kb on + strand at 3824.521 kb on + strand at 3824.522 kb on - strand at 3824.667 kb on + strand, within sfsA at 3824.717 kb on - strand, within sfsA at 3824.799 kb on + strand, within sfsA at 3824.803 kb on - strand, within sfsA at 3824.825 kb on + strand, within sfsA at 3824.897 kb on - strand, within sfsA at 3824.956 kb on + strand, within sfsA at 3824.956 kb on + strand, within sfsA at 3824.958 kb on + strand, within sfsA at 3825.123 kb on + strand, within sfsA at 3825.151 kb on + strand, within sfsA at 3825.202 kb on + strand at 3825.204 kb on + strand at 3825.204 kb on + strand at 3825.204 kb on + strand at 3825.234 kb on - strand at 3825.234 kb on - strand at 3825.262 kb on + strand at 3825.263 kb on - strand at 3825.263 kb on - strand at 3825.268 kb on - strand at 3825.268 kb on - strand at 3825.284 kb on + strand at 3825.312 kb on - strand at 3825.344 kb on - strand at 3825.344 kb on - strand at 3825.344 kb on - strand at 3825.344 kb on - strand at 3825.389 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 3,823,107 - hrpB LU632_RS20065 0.66 -0.4 3,823,211 - hrpB LU632_RS20065 0.70 -0.7 3,823,623 + hrpB LU632_RS20065 0.87 +0.3 3,823,624 - hrpB LU632_RS20065 0.87 -0.4 3,823,640 + hrpB LU632_RS20065 0.87 -0.1 3,823,788 + +0.7 3,823,872 - -0.7 3,823,872 - +0.5 3,823,953 - -0.3 3,823,990 + -0.1 3,823,990 + +0.4 3,823,991 - -0.3 3,824,083 + thpR LU632_RS20070 0.11 -0.3 3,824,083 + thpR LU632_RS20070 0.11 +0.2 3,824,083 + thpR LU632_RS20070 0.11 +0.6 3,824,203 - thpR LU632_RS20070 0.33 -0.3 3,824,398 + thpR LU632_RS20070 0.69 +0.2 3,824,399 - thpR LU632_RS20070 0.69 -1.1 3,824,466 - thpR LU632_RS20070 0.82 +0.9 3,824,485 - thpR LU632_RS20070 0.85 -0.5 3,824,519 + +2.5 3,824,521 + -0.3 3,824,521 + -0.1 3,824,521 + -0.0 3,824,522 - -0.7 3,824,667 + sfsA LU632_RS20075 0.15 -0.2 3,824,717 - sfsA LU632_RS20075 0.22 -1.9 3,824,799 + sfsA LU632_RS20075 0.34 +0.0 3,824,803 - sfsA LU632_RS20075 0.34 -0.1 3,824,825 + sfsA LU632_RS20075 0.38 +0.2 3,824,897 - sfsA LU632_RS20075 0.48 -0.1 3,824,956 + sfsA LU632_RS20075 0.56 +0.4 3,824,956 + sfsA LU632_RS20075 0.56 +0.0 3,824,958 + sfsA LU632_RS20075 0.56 -1.7 3,825,123 + sfsA LU632_RS20075 0.80 +0.1 3,825,151 + sfsA LU632_RS20075 0.84 -0.1 3,825,202 + +0.7 3,825,204 + +0.1 3,825,204 + +0.1 3,825,204 + -0.3 3,825,234 - +0.6 3,825,234 - +0.1 3,825,262 + +0.0 3,825,263 - +0.1 3,825,263 - +0.5 3,825,268 - +0.5 3,825,268 - -0.1 3,825,284 + +0.1 3,825,312 - +0.5 3,825,344 - +0.8 3,825,344 - +0.7 3,825,344 - +0.7 3,825,344 - +1.0 3,825,389 + +1.2
Or see this region's nucleotide sequence