Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS17000 and LU632_RS17005 are separated by 40 nucleotides LU632_RS17005 and tnpA are separated by 170 nucleotides tnpA and LU632_RS17015 are separated by 143 nucleotides LU632_RS17015 and LU632_RS17020 overlap by 8 nucleotides LU632_RS17020 and smpB are separated by 158 nucleotides
LU632_RS17000: LU632_RS17000 - IS3 family transposase, at 3,206,062 to 3,207,182
_RS17000
LU632_RS17005: LU632_RS17005 - helix-turn-helix domain-containing protein, at 3,207,223 to 3,207,357
_RS17005
LU632_RS17010: tnpA - IS200/IS605 family transposase, at 3,207,528 to 3,207,968
tnpA
LU632_RS17015: LU632_RS17015 - RnfH family protein, at 3,208,112 to 3,208,399
_RS17015
LU632_RS17020: LU632_RS17020 - type II toxin-antitoxin system RatA family toxin, at 3,208,392 to 3,208,826
_RS17020
LU632_RS17025: smpB - SsrA-binding protein SmpB, at 3,208,985 to 3,209,467
smpB
Position (kb)
3208
3209 Strain fitness (log2 ratio)
-2
-1
0
1 at 3207.189 kb on + strand at 3207.189 kb on + strand at 3207.193 kb on + strand at 3207.193 kb on + strand at 3208.088 kb on + strand at 3208.095 kb on + strand at 3208.157 kb on - strand, within LU632_RS17015 at 3208.321 kb on + strand, within LU632_RS17015 at 3208.602 kb on - strand, within LU632_RS17020 at 3208.711 kb on - strand, within LU632_RS17020 at 3208.765 kb on - strand, within LU632_RS17020 at 3208.793 kb on - strand at 3208.827 kb on - strand at 3208.862 kb on - strand at 3208.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 3,207,189 + +0.9 3,207,189 + +0.3 3,207,193 + +0.1 3,207,193 + -0.1 3,208,088 + -1.0 3,208,095 + -0.6 3,208,157 - LU632_RS17015 0.16 -2.0 3,208,321 + LU632_RS17015 0.73 -0.1 3,208,602 - LU632_RS17020 0.48 +1.5 3,208,711 - LU632_RS17020 0.73 -0.9 3,208,765 - LU632_RS17020 0.86 +1.3 3,208,793 - -0.3 3,208,827 - +0.2 3,208,862 - +0.6 3,208,915 - -1.0
Or see this region's nucleotide sequence