Strain Fitness in Erwinia tracheiphila SCR3 around LU632_RS16175

Experiment: MJM9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpgm and seqA are separated by 30 nucleotidesseqA and ybfF are separated by 258 nucleotides LU632_RS16170: pgm - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), at 3,058,150 to 3,059,790 pgm LU632_RS16175: seqA - replication initiation negative regulator SeqA, at 3,059,821 to 3,060,372 seqA LU632_RS16180: ybfF - esterase, at 3,060,631 to 3,061,395 ybfF Position (kb) 3059 3060 3061Strain fitness (log2 ratio) -1 0 1at 3059.812 kb on - strandat 3059.812 kb on - strandat 3059.816 kb on - strandat 3059.823 kb on - strandat 3059.950 kb on - strand, within seqAat 3059.981 kb on - strand, within seqAat 3060.074 kb on - strand, within seqAat 3060.217 kb on - strand, within seqAat 3060.217 kb on - strand, within seqAat 3060.311 kb on - strand, within seqAat 3060.311 kb on - strand, within seqAat 3060.311 kb on - strand, within seqAat 3060.312 kb on + strand, within seqAat 3060.312 kb on + strand, within seqAat 3060.313 kb on - strand, within seqAat 3060.321 kb on + strandat 3060.336 kb on - strandat 3060.336 kb on - strandat 3060.475 kb on - strandat 3060.510 kb on + strandat 3060.511 kb on - strandat 3060.511 kb on - strandat 3060.531 kb on - strandat 3060.531 kb on - strandat 3060.534 kb on - strandat 3060.553 kb on - strandat 3060.557 kb on + strandat 3060.558 kb on - strandat 3060.558 kb on - strandat 3060.589 kb on + strandat 3060.590 kb on - strandat 3060.599 kb on + strandat 3060.599 kb on + strandat 3060.612 kb on - strandat 3060.820 kb on + strand, within ybfFat 3060.971 kb on + strand, within ybfFat 3060.971 kb on + strand, within ybfFat 3060.972 kb on - strand, within ybfFat 3060.972 kb on - strand, within ybfFat 3060.972 kb on - strand, within ybfFat 3060.972 kb on - strand, within ybfFat 3060.988 kb on - strand, within ybfFat 3060.988 kb on - strand, within ybfFat 3061.074 kb on - strand, within ybfFat 3061.231 kb on - strand, within ybfFat 3061.232 kb on + strand, within ybfFat 3061.252 kb on + strand, within ybfFat 3061.312 kb on - strand, within ybfFat 3061.337 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MJM9
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3,059,812 - -1.4
3,059,812 - -1.0
3,059,816 - -0.6
3,059,823 - -0.3
3,059,950 - seqA LU632_RS16175 0.23 +0.9
3,059,981 - seqA LU632_RS16175 0.29 -1.1
3,060,074 - seqA LU632_RS16175 0.46 +0.5
3,060,217 - seqA LU632_RS16175 0.72 -1.1
3,060,217 - seqA LU632_RS16175 0.72 -1.5
3,060,311 - seqA LU632_RS16175 0.89 -1.4
3,060,311 - seqA LU632_RS16175 0.89 -1.7
3,060,311 - seqA LU632_RS16175 0.89 -0.1
3,060,312 + seqA LU632_RS16175 0.89 -0.1
3,060,312 + seqA LU632_RS16175 0.89 +0.3
3,060,313 - seqA LU632_RS16175 0.89 -1.1
3,060,321 + -1.4
3,060,336 - -1.7
3,060,336 - -1.1
3,060,475 - +0.4
3,060,510 + -1.7
3,060,511 - -0.1
3,060,511 - +0.0
3,060,531 - -0.4
3,060,531 - +0.1
3,060,534 - -0.8
3,060,553 - -0.2
3,060,557 + +0.1
3,060,558 - +0.7
3,060,558 - -0.7
3,060,589 + +0.2
3,060,590 - -0.1
3,060,599 + +0.5
3,060,599 + +0.7
3,060,612 - +0.7
3,060,820 + ybfF LU632_RS16180 0.25 -0.9
3,060,971 + ybfF LU632_RS16180 0.44 -0.2
3,060,971 + ybfF LU632_RS16180 0.44 -0.7
3,060,972 - ybfF LU632_RS16180 0.45 +0.9
3,060,972 - ybfF LU632_RS16180 0.45 +1.2
3,060,972 - ybfF LU632_RS16180 0.45 +0.2
3,060,972 - ybfF LU632_RS16180 0.45 -0.2
3,060,988 - ybfF LU632_RS16180 0.47 -0.9
3,060,988 - ybfF LU632_RS16180 0.47 -0.9
3,061,074 - ybfF LU632_RS16180 0.58 +0.5
3,061,231 - ybfF LU632_RS16180 0.78 -0.3
3,061,232 + ybfF LU632_RS16180 0.79 +0.2
3,061,252 + ybfF LU632_RS16180 0.81 -0.5
3,061,312 - ybfF LU632_RS16180 0.89 -0.4
3,061,337 + +0.7

Or see this region's nucleotide sequence