Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgm and seqA are separated by 30 nucleotides seqA and ybfF are separated by 258 nucleotides
LU632_RS16170: pgm - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), at 3,058,150 to 3,059,790
pgm
LU632_RS16175: seqA - replication initiation negative regulator SeqA, at 3,059,821 to 3,060,372
seqA
LU632_RS16180: ybfF - esterase, at 3,060,631 to 3,061,395
ybfF
Position (kb)
3059
3060
3061 Strain fitness (log2 ratio)
-1
0
1 at 3059.812 kb on - strand at 3059.812 kb on - strand at 3059.816 kb on - strand at 3059.823 kb on - strand at 3059.950 kb on - strand, within seqA at 3059.981 kb on - strand, within seqA at 3060.074 kb on - strand, within seqA at 3060.217 kb on - strand, within seqA at 3060.217 kb on - strand, within seqA at 3060.311 kb on - strand, within seqA at 3060.311 kb on - strand, within seqA at 3060.311 kb on - strand, within seqA at 3060.312 kb on + strand, within seqA at 3060.312 kb on + strand, within seqA at 3060.313 kb on - strand, within seqA at 3060.321 kb on + strand at 3060.336 kb on - strand at 3060.336 kb on - strand at 3060.475 kb on - strand at 3060.510 kb on + strand at 3060.511 kb on - strand at 3060.511 kb on - strand at 3060.531 kb on - strand at 3060.531 kb on - strand at 3060.534 kb on - strand at 3060.553 kb on - strand at 3060.557 kb on + strand at 3060.558 kb on - strand at 3060.558 kb on - strand at 3060.589 kb on + strand at 3060.590 kb on - strand at 3060.599 kb on + strand at 3060.599 kb on + strand at 3060.612 kb on - strand at 3060.820 kb on + strand, within ybfF at 3060.971 kb on + strand, within ybfF at 3060.971 kb on + strand, within ybfF at 3060.972 kb on - strand, within ybfF at 3060.972 kb on - strand, within ybfF at 3060.972 kb on - strand, within ybfF at 3060.972 kb on - strand, within ybfF at 3060.988 kb on - strand, within ybfF at 3060.988 kb on - strand, within ybfF at 3061.074 kb on - strand, within ybfF at 3061.231 kb on - strand, within ybfF at 3061.232 kb on + strand, within ybfF at 3061.252 kb on + strand, within ybfF at 3061.312 kb on - strand, within ybfF at 3061.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 3,059,812 - -1.4 3,059,812 - -1.0 3,059,816 - -0.6 3,059,823 - -0.3 3,059,950 - seqA LU632_RS16175 0.23 +0.9 3,059,981 - seqA LU632_RS16175 0.29 -1.1 3,060,074 - seqA LU632_RS16175 0.46 +0.5 3,060,217 - seqA LU632_RS16175 0.72 -1.1 3,060,217 - seqA LU632_RS16175 0.72 -1.5 3,060,311 - seqA LU632_RS16175 0.89 -1.4 3,060,311 - seqA LU632_RS16175 0.89 -1.7 3,060,311 - seqA LU632_RS16175 0.89 -0.1 3,060,312 + seqA LU632_RS16175 0.89 -0.1 3,060,312 + seqA LU632_RS16175 0.89 +0.3 3,060,313 - seqA LU632_RS16175 0.89 -1.1 3,060,321 + -1.4 3,060,336 - -1.7 3,060,336 - -1.1 3,060,475 - +0.4 3,060,510 + -1.7 3,060,511 - -0.1 3,060,511 - +0.0 3,060,531 - -0.4 3,060,531 - +0.1 3,060,534 - -0.8 3,060,553 - -0.2 3,060,557 + +0.1 3,060,558 - +0.7 3,060,558 - -0.7 3,060,589 + +0.2 3,060,590 - -0.1 3,060,599 + +0.5 3,060,599 + +0.7 3,060,612 - +0.7 3,060,820 + ybfF LU632_RS16180 0.25 -0.9 3,060,971 + ybfF LU632_RS16180 0.44 -0.2 3,060,971 + ybfF LU632_RS16180 0.44 -0.7 3,060,972 - ybfF LU632_RS16180 0.45 +0.9 3,060,972 - ybfF LU632_RS16180 0.45 +1.2 3,060,972 - ybfF LU632_RS16180 0.45 +0.2 3,060,972 - ybfF LU632_RS16180 0.45 -0.2 3,060,988 - ybfF LU632_RS16180 0.47 -0.9 3,060,988 - ybfF LU632_RS16180 0.47 -0.9 3,061,074 - ybfF LU632_RS16180 0.58 +0.5 3,061,231 - ybfF LU632_RS16180 0.78 -0.3 3,061,232 + ybfF LU632_RS16180 0.79 +0.2 3,061,252 + ybfF LU632_RS16180 0.81 -0.5 3,061,312 - ybfF LU632_RS16180 0.89 -0.4 3,061,337 + +0.7
Or see this region's nucleotide sequence