Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS13445 and LU632_RS13450 are separated by 122 nucleotides LU632_RS13450 and cmoA are separated by 138 nucleotides cmoA and cmoB overlap by 4 nucleotides
LU632_RS13445: LU632_RS13445 - DUF72 domain-containing protein, at 2,565,512 to 2,566,336
_RS13445
LU632_RS13450: LU632_RS13450 - MAPEG family protein, at 2,566,459 to 2,566,746
_RS13450
LU632_RS13455: cmoA - carboxy-S-adenosyl-L-methionine synthase CmoA, at 2,566,885 to 2,567,625
cmoA
LU632_RS13460: cmoB - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 2,567,622 to 2,568,590
cmoB
Position (kb)
2566
2567 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2565.633 kb on - strand, within LU632_RS13445 at 2565.809 kb on + strand, within LU632_RS13445 at 2565.809 kb on + strand, within LU632_RS13445 at 2566.252 kb on - strand, within LU632_RS13445 at 2566.340 kb on + strand at 2566.343 kb on - strand at 2566.343 kb on - strand at 2566.373 kb on + strand at 2566.373 kb on + strand at 2566.374 kb on - strand at 2566.437 kb on + strand at 2566.443 kb on + strand at 2566.454 kb on - strand at 2566.454 kb on - strand at 2566.480 kb on - strand at 2566.489 kb on + strand, within LU632_RS13450 at 2566.489 kb on + strand, within LU632_RS13450 at 2566.490 kb on - strand, within LU632_RS13450 at 2566.490 kb on - strand, within LU632_RS13450 at 2566.519 kb on + strand, within LU632_RS13450 at 2566.675 kb on - strand, within LU632_RS13450 at 2566.675 kb on - strand, within LU632_RS13450 at 2566.707 kb on + strand, within LU632_RS13450 at 2566.707 kb on + strand, within LU632_RS13450 at 2566.708 kb on - strand, within LU632_RS13450 at 2566.720 kb on + strand at 2566.720 kb on + strand at 2566.721 kb on - strand at 2566.721 kb on - strand at 2566.781 kb on + strand at 2566.781 kb on + strand at 2566.782 kb on - strand at 2566.782 kb on - strand at 2566.884 kb on + strand at 2566.884 kb on + strand at 2566.885 kb on - strand at 2566.885 kb on - strand at 2566.885 kb on - strand at 2566.885 kb on - strand at 2566.885 kb on - strand at 2567.005 kb on - strand, within cmoA at 2567.005 kb on - strand, within cmoA at 2567.010 kb on + strand, within cmoA at 2567.065 kb on + strand, within cmoA at 2567.072 kb on + strand, within cmoA at 2567.331 kb on + strand, within cmoA at 2567.332 kb on - strand, within cmoA at 2567.332 kb on - strand, within cmoA at 2567.554 kb on - strand at 2567.643 kb on + strand at 2567.725 kb on + strand, within cmoB at 2567.726 kb on - strand, within cmoB
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,565,633 - LU632_RS13445 0.15 -0.0 2,565,809 + LU632_RS13445 0.36 -0.0 2,565,809 + LU632_RS13445 0.36 +1.8 2,566,252 - LU632_RS13445 0.90 -0.1 2,566,340 + -0.8 2,566,343 - -0.0 2,566,343 - -0.6 2,566,373 + +0.7 2,566,373 + -0.0 2,566,374 - +0.0 2,566,437 + +0.4 2,566,443 + +0.5 2,566,454 - +0.2 2,566,454 - +0.1 2,566,480 - -1.1 2,566,489 + LU632_RS13450 0.10 +0.2 2,566,489 + LU632_RS13450 0.10 +0.0 2,566,490 - LU632_RS13450 0.11 +0.4 2,566,490 - LU632_RS13450 0.11 -0.9 2,566,519 + LU632_RS13450 0.21 -0.4 2,566,675 - LU632_RS13450 0.75 +0.6 2,566,675 - LU632_RS13450 0.75 -0.1 2,566,707 + LU632_RS13450 0.86 -0.8 2,566,707 + LU632_RS13450 0.86 +1.0 2,566,708 - LU632_RS13450 0.86 +1.3 2,566,720 + -1.0 2,566,720 + -0.0 2,566,721 - -0.0 2,566,721 - -0.7 2,566,781 + +0.2 2,566,781 + -2.0 2,566,782 - +1.0 2,566,782 - -0.1 2,566,884 + -1.4 2,566,884 + -1.4 2,566,885 - -0.4 2,566,885 - -0.7 2,566,885 - +0.4 2,566,885 - -0.6 2,566,885 - -0.4 2,567,005 - cmoA LU632_RS13455 0.16 -0.0 2,567,005 - cmoA LU632_RS13455 0.16 +1.0 2,567,010 + cmoA LU632_RS13455 0.17 -0.4 2,567,065 + cmoA LU632_RS13455 0.24 -0.7 2,567,072 + cmoA LU632_RS13455 0.25 -1.2 2,567,331 + cmoA LU632_RS13455 0.60 +1.6 2,567,332 - cmoA LU632_RS13455 0.60 -1.5 2,567,332 - cmoA LU632_RS13455 0.60 -0.0 2,567,554 - -0.3 2,567,643 + -0.8 2,567,725 + cmoB LU632_RS13460 0.11 +0.6 2,567,726 - cmoB LU632_RS13460 0.11 +0.2
Or see this region's nucleotide sequence