Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS13275 and LU632_RS13280 are separated by 60 nucleotides LU632_RS13280 and LU632_RS13285 overlap by 39 nucleotides LU632_RS13285 and LU632_RS13290 are separated by 245 nucleotides LU632_RS13290 and copD are separated by 63 nucleotides copD and yobA are separated by 1 nucleotides yobA and LU632_RS13305 are separated by 160 nucleotides LU632_RS13305 and LU632_RS13310 are separated by 84 nucleotides
LU632_RS13275: LU632_RS13275 - DUF1493 family protein, at 2,535,490 to 2,535,837
_RS13275
LU632_RS13280: LU632_RS13280 - DUF2184 domain-containing protein, at 2,535,898 to 2,536,041
_RS13280
LU632_RS13285: LU632_RS13285 - phage integrase, at 2,536,003 to 2,536,140
_RS13285
LU632_RS13290: LU632_RS13290 - YebY family protein, at 2,536,386 to 2,536,736
_RS13290
LU632_RS13295: copD - copper homeostasis membrane protein CopD, at 2,536,800 to 2,537,666
copD
LU632_RS13300: yobA - CopC domain-containing protein YobA, at 2,537,668 to 2,538,039
yobA
LU632_RS13305: LU632_RS13305 - DNA polymerase III subunit theta, at 2,538,200 to 2,538,430
_RS13305
LU632_RS13310: LU632_RS13310 - carbon-nitrogen hydrolase family protein, at 2,538,515 to 2,539,276
_RS13310
Position (kb)
2536
2537
2538 Strain fitness (log2 ratio)
-2
-1
0
1 at 2535.814 kb on - strand at 2535.814 kb on - strand at 2535.870 kb on + strand at 2535.871 kb on - strand at 2535.880 kb on + strand at 2535.913 kb on + strand, within LU632_RS13280 at 2536.078 kb on - strand, within LU632_RS13285 at 2536.126 kb on + strand, within LU632_RS13285 at 2536.305 kb on - strand at 2536.310 kb on + strand at 2536.319 kb on + strand at 2536.324 kb on - strand at 2536.408 kb on - strand at 2536.589 kb on + strand, within LU632_RS13290 at 2536.740 kb on + strand at 2536.767 kb on + strand at 2536.767 kb on + strand at 2536.768 kb on - strand at 2536.874 kb on + strand at 2536.874 kb on + strand at 2536.992 kb on - strand, within copD at 2536.993 kb on + strand, within copD at 2537.029 kb on - strand, within copD at 2537.076 kb on - strand, within copD at 2537.109 kb on + strand, within copD at 2537.158 kb on - strand, within copD at 2537.205 kb on + strand, within copD at 2537.205 kb on + strand, within copD at 2537.268 kb on + strand, within copD at 2537.468 kb on + strand, within copD at 2537.468 kb on + strand, within copD at 2537.469 kb on - strand, within copD at 2537.469 kb on - strand, within copD at 2537.532 kb on - strand, within copD at 2537.532 kb on - strand, within copD at 2537.592 kb on - strand at 2537.592 kb on - strand at 2537.595 kb on - strand at 2537.595 kb on - strand at 2537.602 kb on - strand at 2537.647 kb on + strand at 2537.648 kb on - strand at 2537.648 kb on - strand at 2537.667 kb on - strand at 2537.674 kb on - strand at 2537.687 kb on + strand at 2537.688 kb on - strand at 2537.704 kb on - strand at 2537.742 kb on - strand, within yobA at 2537.787 kb on + strand, within yobA at 2537.875 kb on + strand, within yobA at 2537.875 kb on + strand, within yobA at 2537.875 kb on + strand, within yobA at 2537.875 kb on + strand, within yobA at 2537.875 kb on + strand, within yobA at 2537.876 kb on - strand, within yobA at 2537.876 kb on - strand, within yobA at 2537.876 kb on - strand, within yobA at 2537.876 kb on - strand, within yobA at 2538.027 kb on + strand at 2538.102 kb on - strand at 2538.155 kb on + strand at 2538.156 kb on - strand at 2538.156 kb on - strand at 2538.171 kb on - strand at 2538.198 kb on - strand at 2538.198 kb on - strand at 2538.209 kb on - strand at 2538.291 kb on - strand, within LU632_RS13305 at 2538.295 kb on + strand, within LU632_RS13305 at 2538.296 kb on - strand, within LU632_RS13305 at 2538.296 kb on - strand, within LU632_RS13305 at 2538.429 kb on - strand at 2538.447 kb on + strand at 2538.447 kb on + strand at 2538.457 kb on + strand at 2538.457 kb on + strand at 2538.482 kb on + strand at 2538.625 kb on + strand, within LU632_RS13310
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,535,814 - +0.0 2,535,814 - +0.5 2,535,870 + -0.3 2,535,871 - +1.6 2,535,880 + +0.1 2,535,913 + LU632_RS13280 0.10 +0.3 2,536,078 - LU632_RS13285 0.54 +1.0 2,536,126 + LU632_RS13285 0.89 -0.4 2,536,305 - +0.3 2,536,310 + +0.2 2,536,319 + -0.2 2,536,324 - -0.0 2,536,408 - -1.0 2,536,589 + LU632_RS13290 0.58 -1.8 2,536,740 + -0.3 2,536,767 + +0.5 2,536,767 + +0.2 2,536,768 - +0.2 2,536,874 + +0.9 2,536,874 + -0.0 2,536,992 - copD LU632_RS13295 0.22 +0.6 2,536,993 + copD LU632_RS13295 0.22 -0.2 2,537,029 - copD LU632_RS13295 0.26 +0.0 2,537,076 - copD LU632_RS13295 0.32 -2.0 2,537,109 + copD LU632_RS13295 0.36 +0.1 2,537,158 - copD LU632_RS13295 0.41 +0.4 2,537,205 + copD LU632_RS13295 0.47 +1.4 2,537,205 + copD LU632_RS13295 0.47 +1.0 2,537,268 + copD LU632_RS13295 0.54 -0.0 2,537,468 + copD LU632_RS13295 0.77 +0.2 2,537,468 + copD LU632_RS13295 0.77 +0.2 2,537,469 - copD LU632_RS13295 0.77 +0.6 2,537,469 - copD LU632_RS13295 0.77 +0.0 2,537,532 - copD LU632_RS13295 0.84 +0.6 2,537,532 - copD LU632_RS13295 0.84 +0.6 2,537,592 - +0.7 2,537,592 - +0.8 2,537,595 - +1.0 2,537,595 - -0.0 2,537,602 - -0.1 2,537,647 + -0.4 2,537,648 - +0.4 2,537,648 - -0.8 2,537,667 - -0.8 2,537,674 - -2.0 2,537,687 + -0.6 2,537,688 - -0.0 2,537,704 - -0.5 2,537,742 - yobA LU632_RS13300 0.20 -0.2 2,537,787 + yobA LU632_RS13300 0.32 +0.5 2,537,875 + yobA LU632_RS13300 0.56 -0.5 2,537,875 + yobA LU632_RS13300 0.56 -0.2 2,537,875 + yobA LU632_RS13300 0.56 +0.7 2,537,875 + yobA LU632_RS13300 0.56 +1.3 2,537,875 + yobA LU632_RS13300 0.56 -0.6 2,537,876 - yobA LU632_RS13300 0.56 +0.1 2,537,876 - yobA LU632_RS13300 0.56 -0.6 2,537,876 - yobA LU632_RS13300 0.56 -0.4 2,537,876 - yobA LU632_RS13300 0.56 -0.4 2,538,027 + +0.2 2,538,102 - -0.4 2,538,155 + -0.9 2,538,156 - -1.4 2,538,156 - -1.0 2,538,171 - -0.0 2,538,198 - +1.0 2,538,198 - -0.9 2,538,209 - -0.0 2,538,291 - LU632_RS13305 0.39 +0.0 2,538,295 + LU632_RS13305 0.41 -0.1 2,538,296 - LU632_RS13305 0.42 +0.2 2,538,296 - LU632_RS13305 0.42 +1.5 2,538,429 - +0.6 2,538,447 + -0.1 2,538,447 + +0.4 2,538,457 + +0.3 2,538,457 + -0.4 2,538,482 + +0.3 2,538,625 + LU632_RS13310 0.14 +0.6
Or see this region's nucleotide sequence