Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minD and minE are separated by 3 nucleotides minE and rnd are separated by 51 nucleotides rnd and fadD are separated by 98 nucleotides
LU632_RS13120: minD - septum site-determining protein MinD, at 2,504,011 to 2,504,823
minD
LU632_RS13125: minE - cell division topological specificity factor MinE, at 2,504,827 to 2,505,096
minE
LU632_RS13130: rnd - ribonuclease D, at 2,505,148 to 2,506,266
rnd
LU632_RS13135: fadD - long-chain-fatty-acid--CoA ligase FadD, at 2,506,365 to 2,508,037
fadD
Position (kb)
2505
2506
2507 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2504.153 kb on + strand, within minD at 2504.154 kb on - strand, within minD at 2504.231 kb on + strand, within minD at 2504.359 kb on - strand, within minD at 2504.430 kb on + strand, within minD at 2504.430 kb on + strand, within minD at 2504.430 kb on + strand, within minD at 2504.431 kb on - strand, within minD at 2504.557 kb on + strand, within minD at 2504.558 kb on - strand, within minD at 2504.587 kb on - strand, within minD at 2504.592 kb on - strand, within minD at 2504.592 kb on - strand, within minD at 2504.606 kb on + strand, within minD at 2504.607 kb on - strand, within minD at 2504.618 kb on + strand, within minD at 2504.718 kb on + strand, within minD at 2504.719 kb on - strand, within minD at 2504.979 kb on + strand, within minE at 2504.984 kb on - strand, within minE at 2505.111 kb on + strand at 2505.128 kb on + strand at 2505.129 kb on - strand at 2505.357 kb on + strand, within rnd at 2505.359 kb on + strand, within rnd at 2505.359 kb on + strand, within rnd at 2505.516 kb on + strand, within rnd at 2505.570 kb on + strand, within rnd at 2505.571 kb on - strand, within rnd at 2505.617 kb on - strand, within rnd at 2505.641 kb on - strand, within rnd at 2505.641 kb on - strand, within rnd at 2505.657 kb on + strand, within rnd at 2506.044 kb on + strand, within rnd at 2506.044 kb on + strand, within rnd at 2506.044 kb on + strand, within rnd at 2506.048 kb on - strand, within rnd at 2506.155 kb on - strand at 2506.157 kb on + strand at 2506.157 kb on + strand at 2506.158 kb on - strand at 2506.161 kb on + strand at 2506.161 kb on + strand at 2506.162 kb on - strand at 2506.252 kb on + strand at 2506.253 kb on - strand at 2506.349 kb on + strand at 2506.353 kb on + strand at 2506.401 kb on + strand at 2506.440 kb on + strand at 2506.510 kb on - strand at 2506.644 kb on + strand, within fadD at 2506.645 kb on - strand, within fadD at 2506.677 kb on + strand, within fadD at 2506.732 kb on - strand, within fadD at 2506.762 kb on + strand, within fadD at 2506.763 kb on - strand, within fadD at 2506.822 kb on - strand, within fadD at 2506.929 kb on + strand, within fadD at 2506.934 kb on + strand, within fadD at 2506.935 kb on - strand, within fadD at 2506.957 kb on - strand, within fadD at 2506.987 kb on + strand, within fadD at 2506.987 kb on + strand, within fadD at 2506.988 kb on - strand, within fadD at 2506.991 kb on + strand, within fadD at 2506.992 kb on - strand, within fadD at 2507.124 kb on + strand, within fadD at 2507.124 kb on + strand, within fadD at 2507.124 kb on + strand, within fadD at 2507.125 kb on - strand, within fadD
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,504,153 + minD LU632_RS13120 0.17 -0.6 2,504,154 - minD LU632_RS13120 0.18 -0.7 2,504,231 + minD LU632_RS13120 0.27 +0.1 2,504,359 - minD LU632_RS13120 0.43 +0.6 2,504,430 + minD LU632_RS13120 0.52 -0.2 2,504,430 + minD LU632_RS13120 0.52 +0.7 2,504,430 + minD LU632_RS13120 0.52 +1.0 2,504,431 - minD LU632_RS13120 0.52 +0.3 2,504,557 + minD LU632_RS13120 0.67 -0.6 2,504,558 - minD LU632_RS13120 0.67 -0.5 2,504,587 - minD LU632_RS13120 0.71 -0.2 2,504,592 - minD LU632_RS13120 0.71 +0.1 2,504,592 - minD LU632_RS13120 0.71 -0.2 2,504,606 + minD LU632_RS13120 0.73 +0.2 2,504,607 - minD LU632_RS13120 0.73 +0.3 2,504,618 + minD LU632_RS13120 0.75 -1.0 2,504,718 + minD LU632_RS13120 0.87 +0.1 2,504,719 - minD LU632_RS13120 0.87 +2.6 2,504,979 + minE LU632_RS13125 0.56 -0.0 2,504,984 - minE LU632_RS13125 0.58 -1.0 2,505,111 + +0.1 2,505,128 + -0.4 2,505,129 - -0.2 2,505,357 + rnd LU632_RS13130 0.19 -0.5 2,505,359 + rnd LU632_RS13130 0.19 +2.0 2,505,359 + rnd LU632_RS13130 0.19 +0.1 2,505,516 + rnd LU632_RS13130 0.33 -0.4 2,505,570 + rnd LU632_RS13130 0.38 -3.2 2,505,571 - rnd LU632_RS13130 0.38 -0.5 2,505,617 - rnd LU632_RS13130 0.42 -0.9 2,505,641 - rnd LU632_RS13130 0.44 +0.2 2,505,641 - rnd LU632_RS13130 0.44 -0.6 2,505,657 + rnd LU632_RS13130 0.45 +0.8 2,506,044 + rnd LU632_RS13130 0.80 -1.0 2,506,044 + rnd LU632_RS13130 0.80 +2.0 2,506,044 + rnd LU632_RS13130 0.80 +1.0 2,506,048 - rnd LU632_RS13130 0.80 +0.6 2,506,155 - -0.2 2,506,157 + +0.0 2,506,157 + +0.2 2,506,158 - -0.1 2,506,161 + -0.1 2,506,161 + -0.0 2,506,162 - -0.8 2,506,252 + +0.6 2,506,253 - +0.6 2,506,349 + +0.2 2,506,353 + -0.2 2,506,401 + -0.3 2,506,440 + -0.0 2,506,510 - +0.5 2,506,644 + fadD LU632_RS13135 0.17 +0.2 2,506,645 - fadD LU632_RS13135 0.17 -0.4 2,506,677 + fadD LU632_RS13135 0.19 -0.3 2,506,732 - fadD LU632_RS13135 0.22 -0.0 2,506,762 + fadD LU632_RS13135 0.24 -0.0 2,506,763 - fadD LU632_RS13135 0.24 -0.1 2,506,822 - fadD LU632_RS13135 0.27 -1.1 2,506,929 + fadD LU632_RS13135 0.34 +0.4 2,506,934 + fadD LU632_RS13135 0.34 +1.0 2,506,935 - fadD LU632_RS13135 0.34 +0.3 2,506,957 - fadD LU632_RS13135 0.35 +0.2 2,506,987 + fadD LU632_RS13135 0.37 +0.6 2,506,987 + fadD LU632_RS13135 0.37 -0.3 2,506,988 - fadD LU632_RS13135 0.37 +1.0 2,506,991 + fadD LU632_RS13135 0.37 -0.3 2,506,992 - fadD LU632_RS13135 0.37 +0.7 2,507,124 + fadD LU632_RS13135 0.45 -0.2 2,507,124 + fadD LU632_RS13135 0.45 -0.1 2,507,124 + fadD LU632_RS13135 0.45 +1.0 2,507,125 - fadD LU632_RS13135 0.45 +1.5
Or see this region's nucleotide sequence