Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnpA and LU632_RS13025 are separated by 85 nucleotides LU632_RS13025 and LU632_RS13030 are separated by 100 nucleotides LU632_RS13030 and LU632_RS13035 are separated by 1 nucleotides LU632_RS13035 and LU632_RS13040 are separated by 449 nucleotides
LU632_RS13020: tnpA - IS200/IS605 family transposase, at 2,484,898 to 2,485,302
tnpA
LU632_RS13025: LU632_RS13025 - transposase, at 2,485,388 to 2,485,681
_RS13025
LU632_RS13030: LU632_RS13030 - helix-turn-helix domain-containing protein, at 2,485,782 to 2,486,195
_RS13030
LU632_RS13035: LU632_RS13035 - transposase, at 2,486,197 to 2,486,754
_RS13035
LU632_RS13040: LU632_RS13040 - potassium/proton antiporter, at 2,487,204 to 2,488,931
_RS13040
Position (kb)
2486
2487 Strain fitness (log2 ratio)
-1
0
1 at 2485.375 kb on - strand at 2486.192 kb on + strand at 2486.831 kb on - strand at 2486.981 kb on + strand at 2487.058 kb on + strand at 2487.119 kb on - strand at 2487.222 kb on + strand at 2487.223 kb on - strand at 2487.223 kb on - strand at 2487.376 kb on - strand at 2487.387 kb on + strand, within LU632_RS13040 at 2487.422 kb on - strand, within LU632_RS13040 at 2487.557 kb on + strand, within LU632_RS13040 at 2487.557 kb on + strand, within LU632_RS13040 at 2487.559 kb on + strand, within LU632_RS13040 at 2487.560 kb on - strand, within LU632_RS13040 at 2487.560 kb on - strand, within LU632_RS13040 at 2487.725 kb on + strand, within LU632_RS13040 at 2487.725 kb on + strand, within LU632_RS13040
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,485,375 - -0.2 2,486,192 + -1.0 2,486,831 - +1.0 2,486,981 + -0.2 2,487,058 + -0.4 2,487,119 - +0.0 2,487,222 + +0.4 2,487,223 - -0.0 2,487,223 - -0.4 2,487,376 - -1.1 2,487,387 + LU632_RS13040 0.11 -0.6 2,487,422 - LU632_RS13040 0.13 -0.0 2,487,557 + LU632_RS13040 0.20 +0.5 2,487,557 + LU632_RS13040 0.20 +0.1 2,487,559 + LU632_RS13040 0.21 -0.7 2,487,560 - LU632_RS13040 0.21 -0.3 2,487,560 - LU632_RS13040 0.21 -0.5 2,487,725 + LU632_RS13040 0.30 +1.2 2,487,725 + LU632_RS13040 0.30 -0.1
Or see this region's nucleotide sequence