Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ascB and LU632_RS12235 are separated by 192 nucleotides LU632_RS12235 and LU632_RS12240 are separated by 39 nucleotides LU632_RS12240 and LU632_RS12245 overlap by 82 nucleotides
LU632_RS12230: ascB - 6-phospho-beta-glucosidase, at 2,321,952 to 2,323,345
ascB
LU632_RS12235: LU632_RS12235 - IS256 family transposase, at 2,323,538 to 2,324,575
_RS12235
LU632_RS12240: LU632_RS12240 - IS1 family transposase, at 2,324,615 to 2,325,118
_RS12240
LU632_RS12245: LU632_RS12245 - IS1-like element transposase, at 2,325,037 to 2,325,312
_RS12245
Position (kb)
2323
2324
2325 Strain fitness (log2 ratio)
-2
-1
0
1 at 2322.626 kb on + strand, within ascB at 2322.626 kb on + strand, within ascB at 2322.626 kb on + strand, within ascB at 2322.626 kb on + strand, within ascB at 2322.626 kb on + strand, within ascB at 2322.627 kb on - strand, within ascB at 2322.627 kb on - strand, within ascB at 2322.627 kb on - strand, within ascB at 2322.627 kb on - strand, within ascB at 2322.649 kb on - strand, within ascB at 2322.662 kb on + strand, within ascB at 2322.751 kb on - strand, within ascB at 2322.752 kb on + strand, within ascB at 2322.753 kb on - strand, within ascB at 2322.753 kb on - strand, within ascB at 2322.753 kb on - strand, within ascB at 2322.781 kb on + strand, within ascB at 2322.781 kb on + strand, within ascB at 2322.782 kb on - strand, within ascB at 2322.782 kb on - strand, within ascB at 2322.853 kb on - strand, within ascB at 2322.914 kb on + strand, within ascB at 2322.915 kb on - strand, within ascB at 2322.923 kb on + strand, within ascB at 2323.000 kb on - strand, within ascB at 2323.079 kb on + strand, within ascB at 2323.166 kb on + strand, within ascB at 2323.166 kb on + strand, within ascB at 2323.166 kb on + strand, within ascB at 2323.166 kb on + strand, within ascB at 2323.166 kb on + strand, within ascB at 2323.167 kb on - strand, within ascB at 2323.236 kb on + strand at 2323.236 kb on + strand at 2323.236 kb on + strand at 2323.237 kb on - strand at 2323.284 kb on - strand at 2323.361 kb on - strand at 2323.361 kb on - strand at 2323.379 kb on + strand at 2323.380 kb on - strand at 2323.453 kb on + strand at 2324.581 kb on - strand at 2325.506 kb on + strand at 2325.516 kb on + strand at 2325.516 kb on - strand at 2325.551 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,322,626 + ascB LU632_RS12230 0.48 -0.6 2,322,626 + ascB LU632_RS12230 0.48 +0.3 2,322,626 + ascB LU632_RS12230 0.48 +0.6 2,322,626 + ascB LU632_RS12230 0.48 -0.2 2,322,626 + ascB LU632_RS12230 0.48 +0.6 2,322,627 - ascB LU632_RS12230 0.48 -0.4 2,322,627 - ascB LU632_RS12230 0.48 -1.1 2,322,627 - ascB LU632_RS12230 0.48 +1.1 2,322,627 - ascB LU632_RS12230 0.48 +0.4 2,322,649 - ascB LU632_RS12230 0.50 +0.0 2,322,662 + ascB LU632_RS12230 0.51 +0.6 2,322,751 - ascB LU632_RS12230 0.57 +0.4 2,322,752 + ascB LU632_RS12230 0.57 -0.3 2,322,753 - ascB LU632_RS12230 0.57 -0.6 2,322,753 - ascB LU632_RS12230 0.57 -0.5 2,322,753 - ascB LU632_RS12230 0.57 +0.2 2,322,781 + ascB LU632_RS12230 0.59 +1.0 2,322,781 + ascB LU632_RS12230 0.59 -0.4 2,322,782 - ascB LU632_RS12230 0.60 -0.3 2,322,782 - ascB LU632_RS12230 0.60 +0.1 2,322,853 - ascB LU632_RS12230 0.65 -0.6 2,322,914 + ascB LU632_RS12230 0.69 +0.0 2,322,915 - ascB LU632_RS12230 0.69 -2.0 2,322,923 + ascB LU632_RS12230 0.70 -0.6 2,323,000 - ascB LU632_RS12230 0.75 -0.4 2,323,079 + ascB LU632_RS12230 0.81 +0.2 2,323,166 + ascB LU632_RS12230 0.87 -0.0 2,323,166 + ascB LU632_RS12230 0.87 -0.9 2,323,166 + ascB LU632_RS12230 0.87 -0.5 2,323,166 + ascB LU632_RS12230 0.87 +0.5 2,323,166 + ascB LU632_RS12230 0.87 -0.8 2,323,167 - ascB LU632_RS12230 0.87 -0.4 2,323,236 + +0.2 2,323,236 + +1.6 2,323,236 + -0.2 2,323,237 - -0.5 2,323,284 - -0.3 2,323,361 - -0.3 2,323,361 - -0.3 2,323,379 + +0.7 2,323,380 - -0.1 2,323,453 + +0.2 2,324,581 - -0.1 2,325,506 + -0.4 2,325,516 + -0.5 2,325,516 - -0.5 2,325,551 + +0.2
Or see this region's nucleotide sequence