Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uspE and pntB are separated by 105 nucleotides pntB and pntA are separated by 10 nucleotides
LU632_RS11425: uspE - universal stress protein UspE, at 2,179,474 to 2,180,421
uspE
LU632_RS11430: pntB - Re/Si-specific NAD(P)(+) transhydrogenase subunit beta, at 2,180,527 to 2,181,915
pntB
LU632_RS11435: pntA - Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha, at 2,181,926 to 2,183,458
pntA
Position (kb)
2180
2181
2182 Strain fitness (log2 ratio)
-2
-1
0
1 at 2179.575 kb on + strand, within uspE at 2179.576 kb on - strand, within uspE at 2179.677 kb on + strand, within uspE at 2179.735 kb on - strand, within uspE at 2179.735 kb on - strand, within uspE at 2179.788 kb on - strand, within uspE at 2180.034 kb on - strand, within uspE at 2180.091 kb on + strand, within uspE at 2180.123 kb on - strand, within uspE at 2180.207 kb on + strand, within uspE at 2180.368 kb on - strand at 2180.528 kb on + strand at 2180.530 kb on + strand at 2180.531 kb on - strand at 2180.531 kb on - strand at 2180.740 kb on - strand, within pntB at 2180.845 kb on + strand, within pntB at 2180.845 kb on + strand, within pntB at 2180.853 kb on + strand, within pntB at 2181.114 kb on + strand, within pntB at 2181.142 kb on - strand, within pntB at 2181.142 kb on - strand, within pntB at 2181.142 kb on - strand, within pntB at 2181.143 kb on - strand, within pntB at 2181.147 kb on - strand, within pntB at 2181.147 kb on - strand, within pntB at 2181.147 kb on - strand, within pntB at 2181.147 kb on - strand, within pntB at 2181.253 kb on - strand, within pntB at 2181.328 kb on - strand, within pntB at 2181.387 kb on + strand, within pntB at 2181.388 kb on - strand, within pntB at 2181.427 kb on - strand, within pntB at 2181.600 kb on - strand, within pntB at 2181.887 kb on + strand at 2181.887 kb on + strand at 2181.887 kb on + strand at 2181.887 kb on + strand at 2181.887 kb on + strand at 2181.888 kb on - strand at 2181.898 kb on + strand at 2181.902 kb on - strand at 2182.140 kb on + strand, within pntA at 2182.141 kb on - strand, within pntA at 2182.141 kb on - strand, within pntA at 2182.151 kb on + strand, within pntA at 2182.169 kb on + strand, within pntA at 2182.442 kb on - strand, within pntA at 2182.515 kb on - strand, within pntA at 2182.713 kb on - strand, within pntA
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,179,575 + uspE LU632_RS11425 0.11 +1.4 2,179,576 - uspE LU632_RS11425 0.11 -2.2 2,179,677 + uspE LU632_RS11425 0.21 -0.4 2,179,735 - uspE LU632_RS11425 0.28 -0.4 2,179,735 - uspE LU632_RS11425 0.28 -1.2 2,179,788 - uspE LU632_RS11425 0.33 +1.4 2,180,034 - uspE LU632_RS11425 0.59 -0.2 2,180,091 + uspE LU632_RS11425 0.65 +0.2 2,180,123 - uspE LU632_RS11425 0.68 +0.2 2,180,207 + uspE LU632_RS11425 0.77 -1.0 2,180,368 - -1.6 2,180,528 + -0.5 2,180,530 + -0.4 2,180,531 - -2.0 2,180,531 - +1.0 2,180,740 - pntB LU632_RS11430 0.15 -0.3 2,180,845 + pntB LU632_RS11430 0.23 -0.5 2,180,845 + pntB LU632_RS11430 0.23 +0.4 2,180,853 + pntB LU632_RS11430 0.23 +0.7 2,181,114 + pntB LU632_RS11430 0.42 -0.3 2,181,142 - pntB LU632_RS11430 0.44 -0.0 2,181,142 - pntB LU632_RS11430 0.44 +1.5 2,181,142 - pntB LU632_RS11430 0.44 +1.2 2,181,143 - pntB LU632_RS11430 0.44 -1.0 2,181,147 - pntB LU632_RS11430 0.45 -0.1 2,181,147 - pntB LU632_RS11430 0.45 +0.4 2,181,147 - pntB LU632_RS11430 0.45 -0.2 2,181,147 - pntB LU632_RS11430 0.45 +0.1 2,181,253 - pntB LU632_RS11430 0.52 +0.5 2,181,328 - pntB LU632_RS11430 0.58 -0.4 2,181,387 + pntB LU632_RS11430 0.62 -0.0 2,181,388 - pntB LU632_RS11430 0.62 +0.3 2,181,427 - pntB LU632_RS11430 0.65 -1.6 2,181,600 - pntB LU632_RS11430 0.77 -0.2 2,181,887 + -0.3 2,181,887 + -0.6 2,181,887 + +0.4 2,181,887 + -0.6 2,181,887 + -0.0 2,181,888 - -0.2 2,181,898 + -0.0 2,181,902 - +0.5 2,182,140 + pntA LU632_RS11435 0.14 +0.3 2,182,141 - pntA LU632_RS11435 0.14 -0.3 2,182,141 - pntA LU632_RS11435 0.14 -0.6 2,182,151 + pntA LU632_RS11435 0.15 +1.2 2,182,169 + pntA LU632_RS11435 0.16 +0.6 2,182,442 - pntA LU632_RS11435 0.34 +0.3 2,182,515 - pntA LU632_RS11435 0.38 -1.2 2,182,713 - pntA LU632_RS11435 0.51 +0.5
Or see this region's nucleotide sequence