Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pykF and LU632_RS11150 are separated by 23 nucleotides LU632_RS11150 and LU632_RS11155 are separated by 161 nucleotides LU632_RS11155 and LU632_RS11160 are separated by 9 nucleotides LU632_RS11160 and LU632_RS11165 are separated by 4 nucleotides LU632_RS11165 and LU632_RS11170 are separated by 148 nucleotides
LU632_RS11145: pykF - pyruvate kinase PykF, at 2,133,674 to 2,135,086
pykF
LU632_RS11150: LU632_RS11150 - hypothetical protein, at 2,135,110 to 2,135,280
_RS11150
LU632_RS11155: LU632_RS11155 - tRNA-Val, at 2,135,442 to 2,135,518
_RS11155
LU632_RS11160: LU632_RS11160 - tRNA-Val, at 2,135,528 to 2,135,604
_RS11160
LU632_RS11165: LU632_RS11165 - tRNA-Val, at 2,135,609 to 2,135,685
_RS11165
LU632_RS11170: LU632_RS11170 - MATE family efflux transporter, at 2,135,834 to 2,137,207
_RS11170
Position (kb)
2135
2136 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2134.613 kb on + strand, within pykF at 2134.763 kb on - strand, within pykF at 2134.814 kb on - strand, within pykF at 2134.814 kb on - strand, within pykF at 2134.942 kb on + strand, within pykF at 2135.006 kb on - strand at 2135.095 kb on - strand at 2135.101 kb on + strand at 2135.240 kb on - strand, within LU632_RS11150 at 2135.324 kb on + strand at 2135.374 kb on + strand at 2135.452 kb on + strand, within LU632_RS11155 at 2135.510 kb on + strand, within LU632_RS11155 at 2135.882 kb on - strand at 2135.890 kb on + strand at 2135.933 kb on - strand at 2135.997 kb on + strand, within LU632_RS11170 at 2136.025 kb on + strand, within LU632_RS11170 at 2136.037 kb on + strand, within LU632_RS11170 at 2136.050 kb on - strand, within LU632_RS11170 at 2136.178 kb on - strand, within LU632_RS11170 at 2136.381 kb on - strand, within LU632_RS11170 at 2136.506 kb on + strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.507 kb on - strand, within LU632_RS11170 at 2136.526 kb on + strand, within LU632_RS11170 at 2136.526 kb on + strand, within LU632_RS11170
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 2,134,613 + pykF LU632_RS11145 0.66 +0.4 2,134,763 - pykF LU632_RS11145 0.77 -1.2 2,134,814 - pykF LU632_RS11145 0.81 -0.0 2,134,814 - pykF LU632_RS11145 0.81 -0.8 2,134,942 + pykF LU632_RS11145 0.90 -0.3 2,135,006 - -2.7 2,135,095 - -0.8 2,135,101 + -1.3 2,135,240 - LU632_RS11150 0.76 -1.8 2,135,324 + -0.0 2,135,374 + -0.7 2,135,452 + LU632_RS11155 0.13 +0.8 2,135,510 + LU632_RS11155 0.88 -0.6 2,135,882 - -0.2 2,135,890 + -0.1 2,135,933 - -0.5 2,135,997 + LU632_RS11170 0.12 +0.3 2,136,025 + LU632_RS11170 0.14 -0.2 2,136,037 + LU632_RS11170 0.15 +2.0 2,136,050 - LU632_RS11170 0.16 -0.6 2,136,178 - LU632_RS11170 0.25 -0.7 2,136,381 - LU632_RS11170 0.40 -0.0 2,136,506 + LU632_RS11170 0.49 -0.6 2,136,507 - LU632_RS11170 0.49 -2.1 2,136,507 - LU632_RS11170 0.49 +1.0 2,136,507 - LU632_RS11170 0.49 -0.1 2,136,507 - LU632_RS11170 0.49 +0.3 2,136,526 + LU632_RS11170 0.50 -1.3 2,136,526 + LU632_RS11170 0.50 +0.1
Or see this region's nucleotide sequence