Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS10055 and LU632_RS10060 are separated by 78 nucleotides LU632_RS10060 and LU632_RS10065 are separated by 3 nucleotides LU632_RS10065 and LU632_RS10070 are separated by 36 nucleotides LU632_RS10070 and LU632_RS10075 are separated by 196 nucleotides
LU632_RS10055: LU632_RS10055 - IS481 family transposase, at 1,925,440 to 1,926,480
_RS10055
LU632_RS10060: LU632_RS10060 - DUF2057 family protein, at 1,926,559 to 1,926,921
_RS10060
LU632_RS10065: LU632_RS10065 - IS256 family transposase, at 1,926,925 to 1,928,133
_RS10065
LU632_RS10070: LU632_RS10070 - DUF2057 family protein, at 1,928,170 to 1,928,358
_RS10070
LU632_RS10075: LU632_RS10075 - IS1 family transposase, at 1,928,555 to 1,929,252
_RS10075
Position (kb) 1926
1927
1928
1929 Strain fitness (log2 ratio) -1
0
1 at 1926.628 kb on - strand, within LU632_RS10060 at 1926.628 kb on - strand, within LU632_RS10060 at 1926.669 kb on + strand, within LU632_RS10060 at 1926.729 kb on + strand, within LU632_RS10060 at 1926.763 kb on - strand, within LU632_RS10060 at 1926.785 kb on - strand, within LU632_RS10060 at 1926.862 kb on - strand, within LU632_RS10060 at 1928.146 kb on + strand at 1928.156 kb on + strand at 1928.224 kb on + strand, within LU632_RS10070 at 1928.225 kb on - strand, within LU632_RS10070 at 1928.228 kb on - strand, within LU632_RS10070 at 1928.263 kb on + strand, within LU632_RS10070 at 1928.268 kb on + strand, within LU632_RS10070 at 1928.329 kb on - strand, within LU632_RS10070 at 1928.555 kb on - strand at 1928.555 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 1,926,628 - LU632_RS10060 0.19 -0.5 1,926,628 - LU632_RS10060 0.19 +0.4 1,926,669 + LU632_RS10060 0.30 -0.2 1,926,729 + LU632_RS10060 0.47 -0.9 1,926,763 - LU632_RS10060 0.56 -0.3 1,926,785 - LU632_RS10060 0.62 +0.4 1,926,862 - LU632_RS10060 0.83 -0.6 1,928,146 + -1.0 1,928,156 + -0.5 1,928,224 + LU632_RS10070 0.29 +0.2 1,928,225 - LU632_RS10070 0.29 -0.2 1,928,228 - LU632_RS10070 0.31 -0.3 1,928,263 + LU632_RS10070 0.49 +0.0 1,928,268 + LU632_RS10070 0.52 -0.1 1,928,329 - LU632_RS10070 0.84 -0.4 1,928,555 - -0.1 1,928,555 - +1.5
Or see this region's nucleotide sequence