Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaB and moaC are separated by 3 nucleotides moaC and moaD overlap by 8 nucleotides moaD and moaE are separated by 40 nucleotides moaE and LU632_RS09200 are separated by 276 nucleotides
LU632_RS09180: moaB - molybdenum cofactor biosynthesis protein B, at 1,730,136 to 1,730,651
moaB
LU632_RS09185: moaC - cyclic pyranopterin monophosphate synthase MoaC, at 1,730,655 to 1,731,140
moaC
LU632_RS09190: moaD - molybdopterin synthase sulfur carrier subunit, at 1,731,133 to 1,731,378
moaD
LU632_RS09195: moaE - molybdopterin synthase catalytic subunit MoaE, at 1,731,419 to 1,731,832
moaE
LU632_RS09200: LU632_RS09200 - DUF1615 domain-containing protein, at 1,732,109 to 1,733,221
_RS09200
Position (kb)
1731
1732 Strain fitness (log2 ratio)
-2
-1
0
1 at 1730.159 kb on + strand at 1730.172 kb on - strand at 1730.217 kb on - strand, within moaB at 1730.410 kb on + strand, within moaB at 1730.431 kb on + strand, within moaB at 1730.431 kb on + strand, within moaB at 1730.431 kb on + strand, within moaB at 1730.432 kb on - strand, within moaB at 1730.432 kb on - strand, within moaB at 1730.432 kb on - strand, within moaB at 1730.432 kb on - strand, within moaB at 1730.553 kb on - strand, within moaB at 1730.697 kb on - strand at 1730.697 kb on - strand at 1730.890 kb on + strand, within moaC at 1731.033 kb on + strand, within moaC at 1731.033 kb on + strand, within moaC at 1731.034 kb on - strand, within moaC at 1731.038 kb on + strand, within moaC at 1731.039 kb on - strand, within moaC at 1731.039 kb on - strand, within moaC at 1731.039 kb on - strand, within moaC at 1731.039 kb on - strand, within moaC at 1731.093 kb on - strand at 1731.376 kb on + strand at 1731.376 kb on + strand at 1731.376 kb on + strand at 1731.377 kb on - strand at 1731.539 kb on + strand, within moaE at 1731.830 kb on + strand at 1731.830 kb on + strand at 1732.059 kb on + strand at 1732.156 kb on - strand at 1732.171 kb on + strand at 1732.172 kb on - strand at 1732.186 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 1,730,159 + +1.3 1,730,172 - +1.2 1,730,217 - moaB LU632_RS09180 0.16 +0.8 1,730,410 + moaB LU632_RS09180 0.53 +0.5 1,730,431 + moaB LU632_RS09180 0.57 -0.8 1,730,431 + moaB LU632_RS09180 0.57 -0.7 1,730,431 + moaB LU632_RS09180 0.57 -0.3 1,730,432 - moaB LU632_RS09180 0.57 +0.2 1,730,432 - moaB LU632_RS09180 0.57 -1.1 1,730,432 - moaB LU632_RS09180 0.57 -0.5 1,730,432 - moaB LU632_RS09180 0.57 -0.3 1,730,553 - moaB LU632_RS09180 0.81 +0.6 1,730,697 - -0.1 1,730,697 - -0.2 1,730,890 + moaC LU632_RS09185 0.48 -0.7 1,731,033 + moaC LU632_RS09185 0.78 -0.6 1,731,033 + moaC LU632_RS09185 0.78 -0.4 1,731,034 - moaC LU632_RS09185 0.78 -0.6 1,731,038 + moaC LU632_RS09185 0.79 +0.4 1,731,039 - moaC LU632_RS09185 0.79 -2.7 1,731,039 - moaC LU632_RS09185 0.79 +0.9 1,731,039 - moaC LU632_RS09185 0.79 -0.4 1,731,039 - moaC LU632_RS09185 0.79 -1.1 1,731,093 - -0.3 1,731,376 + -0.2 1,731,376 + -0.4 1,731,376 + -0.6 1,731,377 - +0.7 1,731,539 + moaE LU632_RS09195 0.29 +0.3 1,731,830 + +0.7 1,731,830 + +1.3 1,732,059 + +0.4 1,732,156 - +0.3 1,732,171 + +0.0 1,732,172 - -1.4 1,732,186 + +0.3
Or see this region's nucleotide sequence