Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiD and LU632_RS08595 are separated by 41 nucleotides LU632_RS08595 and LU632_RS08600 are separated by 3 nucleotides LU632_RS08600 and LU632_RS08605 are separated by 125 nucleotides LU632_RS08605 and LU632_RS08610 are separated by 165 nucleotides
LU632_RS08590: thiD - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 1,613,468 to 1,614,268
thiD
LU632_RS08595: LU632_RS08595 - hypothetical protein, at 1,614,310 to 1,614,471
_RS08595
LU632_RS08600: LU632_RS08600 - hypothetical protein, at 1,614,475 to 1,614,687
_RS08600
LU632_RS08605: LU632_RS08605 - hypothetical protein, at 1,614,813 to 1,614,959
_RS08605
LU632_RS08610: LU632_RS08610 - Ig-like domain-containing protein, at 1,615,125 to 1,615,796
_RS08610
Position (kb) 1614
1615 Strain fitness (log2 ratio) -1
0
1
2 at 1613.480 kb on - strand at 1614.076 kb on - strand, within thiD at 1614.165 kb on + strand, within thiD at 1614.166 kb on - strand, within thiD at 1614.166 kb on - strand, within thiD at 1614.681 kb on + strand at 1614.682 kb on - strand at 1614.797 kb on + strand at 1614.797 kb on + strand at 1614.797 kb on + strand at 1614.798 kb on - strand at 1614.798 kb on - strand at 1614.863 kb on + strand, within LU632_RS08605 at 1614.864 kb on - strand, within LU632_RS08605 at 1614.898 kb on + strand, within LU632_RS08605 at 1614.898 kb on + strand, within LU632_RS08605 at 1614.943 kb on - strand, within LU632_RS08605 at 1615.024 kb on - strand at 1615.024 kb on - strand at 1615.033 kb on - strand at 1615.072 kb on + strand at 1615.185 kb on - strand at 1615.249 kb on + strand, within LU632_RS08610 at 1615.250 kb on - strand, within LU632_RS08610 at 1615.250 kb on - strand, within LU632_RS08610 at 1615.250 kb on - strand, within LU632_RS08610 at 1615.250 kb on - strand, within LU632_RS08610 at 1615.298 kb on + strand, within LU632_RS08610 at 1615.299 kb on - strand, within LU632_RS08610 at 1615.299 kb on - strand, within LU632_RS08610 at 1615.299 kb on - strand, within LU632_RS08610 at 1615.341 kb on + strand, within LU632_RS08610 at 1615.342 kb on - strand, within LU632_RS08610 at 1615.349 kb on + strand, within LU632_RS08610 at 1615.385 kb on + strand, within LU632_RS08610 at 1615.397 kb on + strand, within LU632_RS08610 at 1615.398 kb on - strand, within LU632_RS08610 at 1615.398 kb on - strand, within LU632_RS08610 at 1615.399 kb on - strand, within LU632_RS08610 at 1615.402 kb on + strand, within LU632_RS08610 at 1615.403 kb on - strand, within LU632_RS08610 at 1615.462 kb on + strand, within LU632_RS08610 at 1615.604 kb on + strand, within LU632_RS08610 at 1615.604 kb on + strand, within LU632_RS08610 at 1615.604 kb on + strand, within LU632_RS08610 at 1615.605 kb on - strand, within LU632_RS08610 at 1615.605 kb on - strand, within LU632_RS08610 at 1615.631 kb on + strand, within LU632_RS08610 at 1615.631 kb on + strand, within LU632_RS08610 at 1615.632 kb on - strand, within LU632_RS08610
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 1,613,480 - -0.3 1,614,076 - thiD LU632_RS08590 0.76 -0.2 1,614,165 + thiD LU632_RS08590 0.87 +0.3 1,614,166 - thiD LU632_RS08590 0.87 +0.3 1,614,166 - thiD LU632_RS08590 0.87 -0.4 1,614,681 + -0.9 1,614,682 - -0.4 1,614,797 + -0.1 1,614,797 + +0.3 1,614,797 + +0.1 1,614,798 - -0.1 1,614,798 - +0.2 1,614,863 + LU632_RS08605 0.34 -0.7 1,614,864 - LU632_RS08605 0.35 +0.3 1,614,898 + LU632_RS08605 0.58 -0.1 1,614,898 + LU632_RS08605 0.58 +1.2 1,614,943 - LU632_RS08605 0.88 -1.3 1,615,024 - -1.1 1,615,024 - +0.9 1,615,033 - +0.2 1,615,072 + +1.0 1,615,185 - -0.1 1,615,249 + LU632_RS08610 0.18 -0.2 1,615,250 - LU632_RS08610 0.19 -0.6 1,615,250 - LU632_RS08610 0.19 +0.1 1,615,250 - LU632_RS08610 0.19 +0.2 1,615,250 - LU632_RS08610 0.19 +0.3 1,615,298 + LU632_RS08610 0.26 -0.3 1,615,299 - LU632_RS08610 0.26 +0.7 1,615,299 - LU632_RS08610 0.26 +0.1 1,615,299 - LU632_RS08610 0.26 -1.4 1,615,341 + LU632_RS08610 0.32 +0.5 1,615,342 - LU632_RS08610 0.32 -0.9 1,615,349 + LU632_RS08610 0.33 -0.1 1,615,385 + LU632_RS08610 0.39 -0.7 1,615,397 + LU632_RS08610 0.40 +1.9 1,615,398 - LU632_RS08610 0.41 -1.5 1,615,398 - LU632_RS08610 0.41 -0.2 1,615,399 - LU632_RS08610 0.41 +0.1 1,615,402 + LU632_RS08610 0.41 -0.5 1,615,403 - LU632_RS08610 0.41 +0.1 1,615,462 + LU632_RS08610 0.50 -0.0 1,615,604 + LU632_RS08610 0.71 +0.3 1,615,604 + LU632_RS08610 0.71 +0.7 1,615,604 + LU632_RS08610 0.71 -0.3 1,615,605 - LU632_RS08610 0.71 +1.2 1,615,605 - LU632_RS08610 0.71 +0.4 1,615,631 + LU632_RS08610 0.75 -0.1 1,615,631 + LU632_RS08610 0.75 +0.9 1,615,632 - LU632_RS08610 0.75 +0.2
Or see this region's nucleotide sequence