Strain Fitness in Erwinia tracheiphila SCR3 around LU632_RS06465

Experiment: MJM9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttruD and surE are separated by 532 nucleotidessurE and LU632_RS06470 overlap by 7 nucleotidesLU632_RS06470 and nlpD are separated by 143 nucleotides LU632_RS06460: truD - tRNA pseudouridine(13) synthase TruD, at 1,241,251 to 1,242,300 truD LU632_RS06465: surE - 5'/3'-nucleotidase SurE, at 1,242,833 to 1,243,594 surE LU632_RS06470: LU632_RS06470 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 1,243,588 to 1,244,214 _RS06470 LU632_RS06475: nlpD - murein hydrolase activator NlpD, at 1,244,358 to 1,245,473 nlpD Position (kb) 1242 1243 1244Strain fitness (log2 ratio) -2 -1 0 1 2at 1241.852 kb on + strand, within truDat 1241.857 kb on - strand, within truDat 1241.998 kb on - strand, within truDat 1241.998 kb on - strand, within truDat 1241.998 kb on - strand, within truDat 1242.159 kb on - strand, within truDat 1242.159 kb on - strand, within truDat 1242.249 kb on - strandat 1242.263 kb on + strandat 1242.360 kb on + strandat 1242.418 kb on + strandat 1242.418 kb on + strandat 1242.418 kb on + strandat 1242.419 kb on - strandat 1242.419 kb on - strandat 1242.459 kb on - strandat 1242.546 kb on + strandat 1242.585 kb on + strandat 1242.586 kb on - strandat 1242.644 kb on + strandat 1242.705 kb on + strandat 1242.716 kb on - strandat 1242.779 kb on + strandat 1242.779 kb on + strandat 1242.779 kb on + strandat 1242.780 kb on - strandat 1242.862 kb on + strandat 1243.063 kb on + strand, within surEat 1243.185 kb on + strand, within surEat 1243.414 kb on + strand, within surEat 1243.592 kb on + strandat 1243.771 kb on + strand, within LU632_RS06470at 1243.771 kb on + strand, within LU632_RS06470at 1243.771 kb on + strand, within LU632_RS06470at 1243.772 kb on - strand, within LU632_RS06470at 1243.773 kb on + strand, within LU632_RS06470at 1243.773 kb on + strand, within LU632_RS06470at 1243.773 kb on + strand, within LU632_RS06470at 1244.011 kb on + strand, within LU632_RS06470at 1244.062 kb on - strand, within LU632_RS06470at 1244.231 kb on + strandat 1244.231 kb on + strandat 1244.279 kb on + strandat 1244.335 kb on + strandat 1244.386 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MJM9
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1,241,852 + truD LU632_RS06460 0.57 +1.6
1,241,857 - truD LU632_RS06460 0.58 +0.5
1,241,998 - truD LU632_RS06460 0.71 -1.1
1,241,998 - truD LU632_RS06460 0.71 -0.1
1,241,998 - truD LU632_RS06460 0.71 +0.3
1,242,159 - truD LU632_RS06460 0.86 +0.5
1,242,159 - truD LU632_RS06460 0.86 -0.9
1,242,249 - -0.6
1,242,263 + +0.1
1,242,360 + +0.2
1,242,418 + -0.6
1,242,418 + +1.5
1,242,418 + +0.7
1,242,419 - +0.3
1,242,419 - -2.6
1,242,459 - +0.9
1,242,546 + -0.0
1,242,585 + +0.3
1,242,586 - -0.2
1,242,644 + +0.1
1,242,705 + -0.9
1,242,716 - -0.5
1,242,779 + +1.1
1,242,779 + -0.4
1,242,779 + +0.4
1,242,780 - -1.2
1,242,862 + -0.1
1,243,063 + surE LU632_RS06465 0.30 -1.3
1,243,185 + surE LU632_RS06465 0.46 -0.1
1,243,414 + surE LU632_RS06465 0.76 -1.4
1,243,592 + -2.1
1,243,771 + LU632_RS06470 0.29 -0.3
1,243,771 + LU632_RS06470 0.29 -0.3
1,243,771 + LU632_RS06470 0.29 -0.1
1,243,772 - LU632_RS06470 0.29 -0.9
1,243,773 + LU632_RS06470 0.30 -0.7
1,243,773 + LU632_RS06470 0.30 -0.1
1,243,773 + LU632_RS06470 0.30 +0.7
1,244,011 + LU632_RS06470 0.67 +0.9
1,244,062 - LU632_RS06470 0.76 +1.9
1,244,231 + +0.5
1,244,231 + -1.1
1,244,279 + -0.5
1,244,335 + -0.3
1,244,386 + -1.1

Or see this region's nucleotide sequence