Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt truD and surE are separated by 532 nucleotides surE and LU632_RS06470 overlap by 7 nucleotides LU632_RS06470 and nlpD are separated by 143 nucleotides
LU632_RS06460: truD - tRNA pseudouridine(13) synthase TruD, at 1,241,251 to 1,242,300
truD
LU632_RS06465: surE - 5'/3'-nucleotidase SurE, at 1,242,833 to 1,243,594
surE
LU632_RS06470: LU632_RS06470 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 1,243,588 to 1,244,214
_RS06470
LU632_RS06475: nlpD - murein hydrolase activator NlpD, at 1,244,358 to 1,245,473
nlpD
Position (kb)
1242
1243
1244 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1241.852 kb on + strand, within truD at 1241.857 kb on - strand, within truD at 1241.998 kb on - strand, within truD at 1241.998 kb on - strand, within truD at 1241.998 kb on - strand, within truD at 1242.159 kb on - strand, within truD at 1242.159 kb on - strand, within truD at 1242.249 kb on - strand at 1242.263 kb on + strand at 1242.360 kb on + strand at 1242.418 kb on + strand at 1242.418 kb on + strand at 1242.418 kb on + strand at 1242.419 kb on - strand at 1242.419 kb on - strand at 1242.459 kb on - strand at 1242.546 kb on + strand at 1242.585 kb on + strand at 1242.586 kb on - strand at 1242.644 kb on + strand at 1242.705 kb on + strand at 1242.716 kb on - strand at 1242.779 kb on + strand at 1242.779 kb on + strand at 1242.779 kb on + strand at 1242.780 kb on - strand at 1242.862 kb on + strand at 1243.063 kb on + strand, within surE at 1243.185 kb on + strand, within surE at 1243.414 kb on + strand, within surE at 1243.592 kb on + strand at 1243.771 kb on + strand, within LU632_RS06470 at 1243.771 kb on + strand, within LU632_RS06470 at 1243.771 kb on + strand, within LU632_RS06470 at 1243.772 kb on - strand, within LU632_RS06470 at 1243.773 kb on + strand, within LU632_RS06470 at 1243.773 kb on + strand, within LU632_RS06470 at 1243.773 kb on + strand, within LU632_RS06470 at 1244.011 kb on + strand, within LU632_RS06470 at 1244.062 kb on - strand, within LU632_RS06470 at 1244.231 kb on + strand at 1244.231 kb on + strand at 1244.279 kb on + strand at 1244.335 kb on + strand at 1244.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 1,241,852 + truD LU632_RS06460 0.57 +1.6 1,241,857 - truD LU632_RS06460 0.58 +0.5 1,241,998 - truD LU632_RS06460 0.71 -1.1 1,241,998 - truD LU632_RS06460 0.71 -0.1 1,241,998 - truD LU632_RS06460 0.71 +0.3 1,242,159 - truD LU632_RS06460 0.86 +0.5 1,242,159 - truD LU632_RS06460 0.86 -0.9 1,242,249 - -0.6 1,242,263 + +0.1 1,242,360 + +0.2 1,242,418 + -0.6 1,242,418 + +1.5 1,242,418 + +0.7 1,242,419 - +0.3 1,242,419 - -2.6 1,242,459 - +0.9 1,242,546 + -0.0 1,242,585 + +0.3 1,242,586 - -0.2 1,242,644 + +0.1 1,242,705 + -0.9 1,242,716 - -0.5 1,242,779 + +1.1 1,242,779 + -0.4 1,242,779 + +0.4 1,242,780 - -1.2 1,242,862 + -0.1 1,243,063 + surE LU632_RS06465 0.30 -1.3 1,243,185 + surE LU632_RS06465 0.46 -0.1 1,243,414 + surE LU632_RS06465 0.76 -1.4 1,243,592 + -2.1 1,243,771 + LU632_RS06470 0.29 -0.3 1,243,771 + LU632_RS06470 0.29 -0.3 1,243,771 + LU632_RS06470 0.29 -0.1 1,243,772 - LU632_RS06470 0.29 -0.9 1,243,773 + LU632_RS06470 0.30 -0.7 1,243,773 + LU632_RS06470 0.30 -0.1 1,243,773 + LU632_RS06470 0.30 +0.7 1,244,011 + LU632_RS06470 0.67 +0.9 1,244,062 - LU632_RS06470 0.76 +1.9 1,244,231 + +0.5 1,244,231 + -1.1 1,244,279 + -0.5 1,244,335 + -0.3 1,244,386 + -1.1
Or see this region's nucleotide sequence