Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt crcB and LU632_RS01325 are separated by 76 nucleotides LU632_RS01325 and LU632_RS01330 are separated by 11 nucleotides LU632_RS01330 and LU632_RS01335 are separated by 6 nucleotides
LU632_RS01320: crcB - fluoride efflux transporter CrcB, at 273,679 to 274,059
crcB
LU632_RS01325: LU632_RS01325 - MFS transporter, at 274,136 to 274,643
_RS01325
LU632_RS01330: LU632_RS01330 - sugar porter family MFS transporter, at 274,655 to 274,951
_RS01330
LU632_RS01335: LU632_RS01335 - MFS transporter, at 274,958 to 275,260
_RS01335
Position (kb)
274
275 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 273.152 kb on - strand at 273.152 kb on - strand at 273.152 kb on - strand at 273.158 kb on - strand at 273.158 kb on - strand at 273.239 kb on - strand at 273.258 kb on + strand at 273.258 kb on + strand at 273.259 kb on - strand at 273.259 kb on - strand at 273.259 kb on - strand at 273.259 kb on - strand at 273.283 kb on - strand at 273.283 kb on - strand at 273.314 kb on - strand at 273.602 kb on + strand at 273.820 kb on - strand, within crcB at 273.897 kb on - strand, within crcB at 273.897 kb on - strand, within crcB at 273.906 kb on + strand, within crcB at 274.057 kb on + strand at 274.084 kb on - strand at 274.084 kb on - strand at 274.084 kb on - strand at 274.132 kb on + strand at 274.202 kb on + strand, within LU632_RS01325 at 274.492 kb on + strand, within LU632_RS01325 at 274.492 kb on + strand, within LU632_RS01325 at 274.530 kb on + strand, within LU632_RS01325 at 274.530 kb on + strand, within LU632_RS01325 at 274.530 kb on + strand, within LU632_RS01325 at 274.534 kb on - strand, within LU632_RS01325 at 274.534 kb on - strand, within LU632_RS01325 at 274.568 kb on + strand, within LU632_RS01325 at 274.625 kb on + strand at 274.648 kb on - strand at 274.671 kb on - strand at 274.671 kb on - strand at 274.728 kb on + strand, within LU632_RS01330 at 274.728 kb on + strand, within LU632_RS01330 at 274.729 kb on - strand, within LU632_RS01330 at 274.729 kb on - strand, within LU632_RS01330 at 274.741 kb on + strand, within LU632_RS01330 at 274.741 kb on + strand, within LU632_RS01330 at 274.744 kb on + strand, within LU632_RS01330 at 274.882 kb on + strand, within LU632_RS01330 at 274.882 kb on + strand, within LU632_RS01330 at 274.883 kb on - strand, within LU632_RS01330 at 274.883 kb on - strand, within LU632_RS01330 at 274.883 kb on - strand, within LU632_RS01330 at 274.883 kb on - strand, within LU632_RS01330 at 274.883 kb on - strand, within LU632_RS01330 at 274.969 kb on + strand at 274.983 kb on - strand at 275.095 kb on + strand, within LU632_RS01335 at 275.095 kb on + strand, within LU632_RS01335 at 275.096 kb on - strand, within LU632_RS01335 at 275.159 kb on + strand, within LU632_RS01335 at 275.176 kb on - strand, within LU632_RS01335 at 275.176 kb on - strand, within LU632_RS01335 at 275.176 kb on - strand, within LU632_RS01335 at 275.221 kb on + strand, within LU632_RS01335 at 275.299 kb on + strand at 275.300 kb on - strand at 275.303 kb on + strand at 275.303 kb on + strand at 275.303 kb on + strand at 275.303 kb on + strand at 275.304 kb on - strand at 275.304 kb on - strand at 275.304 kb on - strand at 275.304 kb on - strand at 275.304 kb on - strand at 275.332 kb on - strand at 275.332 kb on - strand at 275.332 kb on - strand at 275.332 kb on - strand at 275.332 kb on - strand at 275.332 kb on - strand at 275.333 kb on + strand at 275.333 kb on + strand at 275.334 kb on - strand at 275.334 kb on - strand at 275.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 273,152 - -1.0 273,152 - +0.3 273,152 - -0.0 273,158 - +0.0 273,158 - -0.7 273,239 - +0.3 273,258 + -0.4 273,258 + +0.3 273,259 - +0.7 273,259 - -0.1 273,259 - -0.3 273,259 - -0.0 273,283 - -0.8 273,283 - -0.4 273,314 - -2.6 273,602 + +0.0 273,820 - crcB LU632_RS01320 0.37 +0.0 273,897 - crcB LU632_RS01320 0.57 -1.0 273,897 - crcB LU632_RS01320 0.57 -1.4 273,906 + crcB LU632_RS01320 0.60 +0.3 274,057 + -0.3 274,084 - -1.6 274,084 - -0.6 274,084 - +1.2 274,132 + -0.3 274,202 + LU632_RS01325 0.13 -0.3 274,492 + LU632_RS01325 0.70 +0.6 274,492 + LU632_RS01325 0.70 +0.1 274,530 + LU632_RS01325 0.78 +0.3 274,530 + LU632_RS01325 0.78 +0.2 274,530 + LU632_RS01325 0.78 -0.3 274,534 - LU632_RS01325 0.78 +0.2 274,534 - LU632_RS01325 0.78 +0.5 274,568 + LU632_RS01325 0.85 +2.2 274,625 + +0.6 274,648 - -0.4 274,671 - -0.8 274,671 - +0.6 274,728 + LU632_RS01330 0.25 -1.0 274,728 + LU632_RS01330 0.25 -0.1 274,729 - LU632_RS01330 0.25 -0.1 274,729 - LU632_RS01330 0.25 +0.6 274,741 + LU632_RS01330 0.29 -0.2 274,741 + LU632_RS01330 0.29 +0.9 274,744 + LU632_RS01330 0.30 -0.3 274,882 + LU632_RS01330 0.76 +0.2 274,882 + LU632_RS01330 0.76 -1.4 274,883 - LU632_RS01330 0.77 -0.3 274,883 - LU632_RS01330 0.77 -0.2 274,883 - LU632_RS01330 0.77 -0.1 274,883 - LU632_RS01330 0.77 -0.1 274,883 - LU632_RS01330 0.77 -0.3 274,969 + -0.5 274,983 - +0.9 275,095 + LU632_RS01335 0.45 +1.7 275,095 + LU632_RS01335 0.45 -0.2 275,096 - LU632_RS01335 0.46 -0.6 275,159 + LU632_RS01335 0.66 -0.5 275,176 - LU632_RS01335 0.72 -0.6 275,176 - LU632_RS01335 0.72 -0.2 275,176 - LU632_RS01335 0.72 +0.9 275,221 + LU632_RS01335 0.87 -0.3 275,299 + -0.4 275,300 - +0.9 275,303 + -0.3 275,303 + +0.0 275,303 + -1.7 275,303 + +0.7 275,304 - -0.2 275,304 - -0.3 275,304 - +0.0 275,304 - -0.4 275,304 - -0.2 275,332 - +0.2 275,332 - +0.5 275,332 - +0.9 275,332 - +0.1 275,332 - -0.4 275,332 - -0.4 275,333 + -0.3 275,333 + -0.3 275,334 - +0.2 275,334 - -1.0 275,334 - -0.1
Or see this region's nucleotide sequence