Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purD and purH are separated by 14 nucleotides purH and LU632_RS01025 are separated by 590 nucleotides
LU632_RS01015: purD - phosphoribosylamine--glycine ligase, at 217,496 to 218,776
purD
LU632_RS01020: purH - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 218,791 to 220,380
purH
LU632_RS01025: LU632_RS01025 - 16S ribosomal RNA, at 220,971 to 222,512
_RS01025
Position (kb) 218
219
220
221 Strain fitness (log2 ratio) -2
-1
0
1 at 217.833 kb on - strand, within purD at 217.915 kb on + strand, within purD at 218.131 kb on + strand, within purD at 218.132 kb on - strand, within purD at 218.132 kb on - strand, within purD at 218.227 kb on + strand, within purD at 218.227 kb on + strand, within purD at 218.320 kb on + strand, within purD at 218.321 kb on - strand, within purD at 218.366 kb on - strand, within purD at 218.366 kb on - strand, within purD at 218.372 kb on + strand, within purD at 218.373 kb on - strand, within purD at 218.377 kb on - strand, within purD at 218.377 kb on - strand, within purD at 218.621 kb on - strand, within purD at 218.766 kb on - strand at 218.793 kb on - strand at 219.030 kb on + strand, within purH at 219.067 kb on - strand, within purH at 219.100 kb on - strand, within purH at 219.112 kb on + strand, within purH at 219.113 kb on - strand, within purH at 219.193 kb on + strand, within purH at 219.194 kb on - strand, within purH at 219.316 kb on - strand, within purH at 219.334 kb on - strand, within purH at 219.423 kb on + strand, within purH at 219.471 kb on - strand, within purH at 219.597 kb on - strand, within purH at 219.661 kb on + strand, within purH at 219.662 kb on - strand, within purH at 219.662 kb on - strand, within purH at 219.662 kb on - strand, within purH at 219.665 kb on + strand, within purH at 219.665 kb on + strand, within purH at 219.666 kb on - strand, within purH at 219.780 kb on - strand, within purH at 219.803 kb on + strand, within purH at 219.834 kb on + strand, within purH at 219.857 kb on - strand, within purH at 219.891 kb on + strand, within purH at 220.105 kb on + strand, within purH at 220.106 kb on - strand, within purH at 220.388 kb on - strand at 220.409 kb on + strand at 220.410 kb on - strand at 220.414 kb on - strand at 220.422 kb on + strand at 220.423 kb on - strand at 220.650 kb on - strand at 220.650 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 217,833 - purD LU632_RS01015 0.26 -1.2 217,915 + purD LU632_RS01015 0.33 -0.6 218,131 + purD LU632_RS01015 0.50 -1.1 218,132 - purD LU632_RS01015 0.50 -0.4 218,132 - purD LU632_RS01015 0.50 +0.2 218,227 + purD LU632_RS01015 0.57 -0.4 218,227 + purD LU632_RS01015 0.57 -0.3 218,320 + purD LU632_RS01015 0.64 +0.2 218,321 - purD LU632_RS01015 0.64 -0.7 218,366 - purD LU632_RS01015 0.68 -0.1 218,366 - purD LU632_RS01015 0.68 -1.1 218,372 + purD LU632_RS01015 0.68 -0.4 218,373 - purD LU632_RS01015 0.68 -0.1 218,377 - purD LU632_RS01015 0.69 -0.3 218,377 - purD LU632_RS01015 0.69 -0.7 218,621 - purD LU632_RS01015 0.88 -0.1 218,766 - +0.5 218,793 - +0.0 219,030 + purH LU632_RS01020 0.15 -0.3 219,067 - purH LU632_RS01020 0.17 -1.1 219,100 - purH LU632_RS01020 0.19 -0.7 219,112 + purH LU632_RS01020 0.20 -2.1 219,113 - purH LU632_RS01020 0.20 -0.4 219,193 + purH LU632_RS01020 0.25 -0.5 219,194 - purH LU632_RS01020 0.25 -0.7 219,316 - purH LU632_RS01020 0.33 +0.9 219,334 - purH LU632_RS01020 0.34 -1.0 219,423 + purH LU632_RS01020 0.40 -1.6 219,471 - purH LU632_RS01020 0.43 -2.6 219,597 - purH LU632_RS01020 0.51 -1.4 219,661 + purH LU632_RS01020 0.55 +0.6 219,662 - purH LU632_RS01020 0.55 -0.8 219,662 - purH LU632_RS01020 0.55 -0.7 219,662 - purH LU632_RS01020 0.55 +0.3 219,665 + purH LU632_RS01020 0.55 +0.9 219,665 + purH LU632_RS01020 0.55 -0.1 219,666 - purH LU632_RS01020 0.55 +1.5 219,780 - purH LU632_RS01020 0.62 -0.7 219,803 + purH LU632_RS01020 0.64 -0.0 219,834 + purH LU632_RS01020 0.66 -1.0 219,857 - purH LU632_RS01020 0.67 +0.5 219,891 + purH LU632_RS01020 0.69 -1.8 220,105 + purH LU632_RS01020 0.83 -1.9 220,106 - purH LU632_RS01020 0.83 +0.5 220,388 - -0.3 220,409 + -0.3 220,410 - -0.2 220,414 - +0.5 220,422 + -0.1 220,423 - -0.2 220,650 - +0.3 220,650 - -0.2
Or see this region's nucleotide sequence