Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS00875 and LU632_RS00880 are separated by 12 nucleotides LU632_RS00880 and LU632_RS00885 are separated by 147 nucleotides LU632_RS00885 and LU632_RS00890 are separated by 6 nucleotides LU632_RS00890 and tuf are separated by 113 nucleotides tuf and secE are separated by 352 nucleotides secE and nusG are separated by 1 nucleotides
LU632_RS00875: LU632_RS00875 - tRNA-Thr, at 191,221 to 191,296
_RS00875
LU632_RS00880: LU632_RS00880 - tRNA-Tyr, at 191,309 to 191,393
_RS00880
LU632_RS00885: LU632_RS00885 - tRNA-Gly, at 191,541 to 191,615
_RS00885
LU632_RS00890: LU632_RS00890 - tRNA-Thr, at 191,622 to 191,697
_RS00890
LU632_RS00895: tuf - elongation factor Tu, at 191,811 to 192,995
tuf
LU632_RS00900: secE - preprotein translocase subunit SecE, at 193,348 to 193,731
secE
LU632_RS00905: nusG - transcription termination/antitermination protein NusG, at 193,733 to 194,278
nusG
Position (kb) 191
192
193 Strain fitness (log2 ratio) -2
-1
0
1
2 at 190.831 kb on + strand at 190.832 kb on - strand at 190.836 kb on + strand at 190.892 kb on - strand at 190.895 kb on - strand at 190.931 kb on + strand at 190.932 kb on - strand at 190.972 kb on - strand at 191.068 kb on + strand at 191.072 kb on - strand at 191.103 kb on + strand at 191.481 kb on + strand at 191.481 kb on + strand at 191.702 kb on + strand at 191.702 kb on + strand at 191.702 kb on + strand at 191.702 kb on + strand at 191.703 kb on - strand at 191.703 kb on - strand at 191.729 kb on + strand at 191.730 kb on - strand at 191.775 kb on + strand at 192.072 kb on + strand, within tuf at 192.198 kb on + strand, within tuf at 192.198 kb on + strand, within tuf at 192.402 kb on - strand, within tuf at 192.405 kb on + strand, within tuf at 192.405 kb on + strand, within tuf at 192.405 kb on + strand, within tuf at 192.405 kb on + strand, within tuf at 192.405 kb on + strand, within tuf at 192.405 kb on + strand, within tuf at 192.405 kb on + strand, within tuf at 192.405 kb on + strand, within tuf at 192.405 kb on + strand, within tuf at 192.406 kb on - strand, within tuf at 192.406 kb on - strand, within tuf at 192.406 kb on - strand, within tuf at 192.956 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 190,831 + -0.0 190,832 - +0.9 190,836 + -0.5 190,892 - +0.5 190,895 - +0.2 190,931 + +0.2 190,932 - -0.3 190,972 - +0.4 191,068 + -0.3 191,072 - -0.2 191,103 + +1.1 191,481 + -0.1 191,481 + +0.1 191,702 + +0.6 191,702 + -0.8 191,702 + -1.7 191,702 + +0.1 191,703 - +2.5 191,703 - +0.5 191,729 + -0.7 191,730 - -0.1 191,775 + -0.1 192,072 + tuf LU632_RS00895 0.22 -1.3 192,198 + tuf LU632_RS00895 0.33 -0.2 192,198 + tuf LU632_RS00895 0.33 -0.7 192,402 - tuf LU632_RS00895 0.50 +0.3 192,405 + tuf LU632_RS00895 0.50 -0.8 192,405 + tuf LU632_RS00895 0.50 -1.0 192,405 + tuf LU632_RS00895 0.50 -0.4 192,405 + tuf LU632_RS00895 0.50 -0.0 192,405 + tuf LU632_RS00895 0.50 -1.9 192,405 + tuf LU632_RS00895 0.50 +0.5 192,405 + tuf LU632_RS00895 0.50 -1.1 192,405 + tuf LU632_RS00895 0.50 +0.3 192,405 + tuf LU632_RS00895 0.50 -0.3 192,406 - tuf LU632_RS00895 0.50 -0.9 192,406 - tuf LU632_RS00895 0.50 -0.7 192,406 - tuf LU632_RS00895 0.50 -2.4 192,956 + -1.3
Or see this region's nucleotide sequence