Experiment: MJM9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS00505 and sicA are separated by 134 nucleotides sicA and sctE are separated by 14 nucleotides sctE and LU632_RS00520 are separated by 34 nucleotides
LU632_RS00505: LU632_RS00505 - EscU/YscU/HrcU family type III secretion system export apparatus switch protein, at 102,693 to 103,784
_RS00505
LU632_RS00510: sicA - type III secretion system translocator chaperone SicA, at 103,919 to 104,455
sicA
LU632_RS00515: sctE - type III secretion system translocon subunit SctE, at 104,470 to 106,341
sctE
LU632_RS00520: LU632_RS00520 - hypothetical protein, at 106,376 to 107,581
_RS00520
Position (kb)
104
105
106
107 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 103.541 kb on + strand, within LU632_RS00505 at 103.695 kb on - strand at 103.695 kb on - strand at 103.789 kb on + strand at 103.814 kb on - strand at 103.840 kb on - strand at 104.062 kb on + strand, within sicA at 104.213 kb on + strand, within sicA at 104.308 kb on - strand, within sicA at 104.353 kb on + strand, within sicA at 104.636 kb on + strand at 104.829 kb on + strand, within sctE at 104.980 kb on + strand, within sctE at 105.002 kb on - strand, within sctE at 105.116 kb on - strand, within sctE at 105.217 kb on - strand, within sctE at 105.220 kb on + strand, within sctE at 105.335 kb on + strand, within sctE at 105.336 kb on - strand, within sctE at 105.339 kb on + strand, within sctE at 105.381 kb on + strand, within sctE at 105.591 kb on + strand, within sctE at 105.727 kb on - strand, within sctE at 105.812 kb on + strand, within sctE at 105.812 kb on + strand, within sctE at 105.813 kb on - strand, within sctE at 105.845 kb on + strand, within sctE at 105.859 kb on + strand, within sctE at 105.952 kb on - strand, within sctE at 106.247 kb on + strand at 106.414 kb on + strand at 106.683 kb on + strand, within LU632_RS00520 at 106.684 kb on - strand, within LU632_RS00520 at 106.684 kb on - strand, within LU632_RS00520 at 106.738 kb on + strand, within LU632_RS00520 at 106.752 kb on + strand, within LU632_RS00520 at 106.758 kb on + strand, within LU632_RS00520 at 106.774 kb on + strand, within LU632_RS00520 at 106.793 kb on + strand, within LU632_RS00520 at 106.793 kb on + strand, within LU632_RS00520 at 106.794 kb on - strand, within LU632_RS00520 at 106.794 kb on - strand, within LU632_RS00520 at 106.862 kb on - strand, within LU632_RS00520 at 106.927 kb on + strand, within LU632_RS00520 at 106.927 kb on + strand, within LU632_RS00520 at 106.928 kb on - strand, within LU632_RS00520 at 106.931 kb on - strand, within LU632_RS00520 at 106.992 kb on + strand, within LU632_RS00520 at 106.992 kb on + strand, within LU632_RS00520 at 107.011 kb on + strand, within LU632_RS00520 at 107.011 kb on + strand, within LU632_RS00520 at 107.012 kb on - strand, within LU632_RS00520 at 107.060 kb on - strand, within LU632_RS00520 at 107.062 kb on + strand, within LU632_RS00520 at 107.065 kb on + strand, within LU632_RS00520 at 107.065 kb on + strand, within LU632_RS00520 at 107.134 kb on + strand, within LU632_RS00520 at 107.236 kb on - strand, within LU632_RS00520
Per-strain Table
Position Strand Gene LocusTag Fraction MJM9 remove 103,541 + LU632_RS00505 0.78 -0.1 103,695 - -0.6 103,695 - +0.1 103,789 + -0.7 103,814 - +0.8 103,840 - +0.6 104,062 + sicA LU632_RS00510 0.27 +0.3 104,213 + sicA LU632_RS00510 0.55 +0.1 104,308 - sicA LU632_RS00510 0.72 +0.1 104,353 + sicA LU632_RS00510 0.81 +0.2 104,636 + -0.4 104,829 + sctE LU632_RS00515 0.19 +0.5 104,980 + sctE LU632_RS00515 0.27 +0.2 105,002 - sctE LU632_RS00515 0.28 -0.4 105,116 - sctE LU632_RS00515 0.35 +1.3 105,217 - sctE LU632_RS00515 0.40 -0.8 105,220 + sctE LU632_RS00515 0.40 +0.1 105,335 + sctE LU632_RS00515 0.46 -0.1 105,336 - sctE LU632_RS00515 0.46 -0.1 105,339 + sctE LU632_RS00515 0.46 -0.4 105,381 + sctE LU632_RS00515 0.49 -0.1 105,591 + sctE LU632_RS00515 0.60 +0.5 105,727 - sctE LU632_RS00515 0.67 +0.0 105,812 + sctE LU632_RS00515 0.72 -0.6 105,812 + sctE LU632_RS00515 0.72 +0.2 105,813 - sctE LU632_RS00515 0.72 -0.3 105,845 + sctE LU632_RS00515 0.73 -0.0 105,859 + sctE LU632_RS00515 0.74 -0.5 105,952 - sctE LU632_RS00515 0.79 -0.4 106,247 + +0.6 106,414 + -0.5 106,683 + LU632_RS00520 0.25 +0.1 106,684 - LU632_RS00520 0.26 -1.7 106,684 - LU632_RS00520 0.26 -0.4 106,738 + LU632_RS00520 0.30 -0.1 106,752 + LU632_RS00520 0.31 +0.6 106,758 + LU632_RS00520 0.32 -0.5 106,774 + LU632_RS00520 0.33 +2.5 106,793 + LU632_RS00520 0.35 -0.4 106,793 + LU632_RS00520 0.35 -2.4 106,794 - LU632_RS00520 0.35 -1.3 106,794 - LU632_RS00520 0.35 -0.5 106,862 - LU632_RS00520 0.40 +0.5 106,927 + LU632_RS00520 0.46 -0.1 106,927 + LU632_RS00520 0.46 +1.3 106,928 - LU632_RS00520 0.46 -0.7 106,931 - LU632_RS00520 0.46 +0.6 106,992 + LU632_RS00520 0.51 +0.9 106,992 + LU632_RS00520 0.51 -0.1 107,011 + LU632_RS00520 0.53 +0.0 107,011 + LU632_RS00520 0.53 -0.9 107,012 - LU632_RS00520 0.53 -0.1 107,060 - LU632_RS00520 0.57 -0.2 107,062 + LU632_RS00520 0.57 -0.5 107,065 + LU632_RS00520 0.57 +0.2 107,065 + LU632_RS00520 0.57 -0.3 107,134 + LU632_RS00520 0.63 +0.6 107,236 - LU632_RS00520 0.71 +0.0
Or see this region's nucleotide sequence