Strain Fitness in Escherichia coli BL21 around ECD_02396

Experiment: Bas34

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02395 and ECD_02396 are separated by 351 nucleotidesECD_02396 and ECD_02397 are separated by 310 nucleotidesECD_02397 and ECD_02398 are separated by 15 nucleotides ECD_02395: ECD_02395 - cyclic-di-GMP phosphodiesterase, anaerobic, at 2,491,175 to 2,493,418 _02395 ECD_02396: ECD_02396 - uncharacterized protein, at 2,493,770 to 2,493,961 _02396 ECD_02397: ECD_02397 - outer membrane integrity lipoprotein, at 2,494,272 to 2,494,790 _02397 ECD_02398: ECD_02398 - nalidixic acid resistance protein, putative periplasmic protein, at 2,494,806 to 2,495,345 _02398 Position (kb) 2493 2494Strain fitness (log2 ratio) -1 0 1at 2492.922 kb on - strand, within ECD_02395at 2493.045 kb on - strand, within ECD_02395at 2493.047 kb on - strand, within ECD_02395at 2493.047 kb on - strand, within ECD_02395at 2493.727 kb on + strandat 2493.731 kb on + strandat 2493.753 kb on - strandat 2493.831 kb on + strand, within ECD_02396at 2493.872 kb on + strand, within ECD_02396at 2493.872 kb on + strand, within ECD_02396at 2493.872 kb on + strand, within ECD_02396at 2494.421 kb on + strand, within ECD_02397at 2494.421 kb on + strand, within ECD_02397at 2494.608 kb on - strand, within ECD_02397at 2494.671 kb on + strand, within ECD_02397at 2494.740 kb on + strandat 2494.791 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas34
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2,492,922 - ECD_02395 0.78 -0.3
2,493,045 - ECD_02395 0.83 -0.6
2,493,047 - ECD_02395 0.83 +0.9
2,493,047 - ECD_02395 0.83 +0.1
2,493,727 + -0.2
2,493,731 + -0.1
2,493,753 - -0.6
2,493,831 + ECD_02396 0.32 -0.1
2,493,872 + ECD_02396 0.53 -0.0
2,493,872 + ECD_02396 0.53 -0.4
2,493,872 + ECD_02396 0.53 -0.2
2,494,421 + ECD_02397 0.29 -1.6
2,494,421 + ECD_02397 0.29 -0.7
2,494,608 - ECD_02397 0.65 -1.1
2,494,671 + ECD_02397 0.77 +1.4
2,494,740 + +0.4
2,494,791 + -0.3

Or see this region's nucleotide sequence