Strain Fitness in Escherichia coli BL21 around ECD_01166

Experiment: Bas34

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01165 and ECD_01166 are separated by 385 nucleotidesECD_01166 and ECD_01167 are separated by 94 nucleotides ECD_01165: ECD_01165 - alanine racemase, catabolic, PLP-binding, at 1,227,027 to 1,228,097 _01165 ECD_01166: ECD_01166 - putative cation/proton antiporter, at 1,228,483 to 1,230,219 _01166 ECD_01167: ECD_01167 - murein tetrapeptide carboxypeptidase; LD-carboxypeptidase A, at 1,230,314 to 1,231,228 _01167 Position (kb) 1228 1229 1230 1231Strain fitness (log2 ratio) -1 0 1 2at 1227.712 kb on + strand, within ECD_01165at 1230.025 kb on - strand, within ECD_01166at 1230.036 kb on - strand, within ECD_01166at 1230.036 kb on - strand, within ECD_01166at 1230.036 kb on - strand, within ECD_01166at 1230.052 kb on - strandat 1230.155 kb on - strandat 1230.252 kb on + strandat 1230.579 kb on + strand, within ECD_01167at 1230.595 kb on + strand, within ECD_01167at 1231.186 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas34
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1,227,712 + ECD_01165 0.64 +1.1
1,230,025 - ECD_01166 0.89 -0.4
1,230,036 - ECD_01166 0.89 -0.0
1,230,036 - ECD_01166 0.89 +0.1
1,230,036 - ECD_01166 0.89 +0.6
1,230,052 - +1.1
1,230,155 - +1.9
1,230,252 + -1.6
1,230,579 + ECD_01167 0.29 +0.8
1,230,595 + ECD_01167 0.31 +0.8
1,231,186 + +0.3

Or see this region's nucleotide sequence