Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS05830 and ABIE41_RS05835 are separated by 17 nucleotides ABIE41_RS05835 and ABIE41_RS05840 are separated by 87 nucleotides ABIE41_RS05840 and ABIE41_RS05845 are separated by 278 nucleotides
ABIE41_RS05830: ABIE41_RS05830 - zinc ABC transporter ATP-binding protein AztA, at 1,199,533 to 1,200,267
_RS05830
ABIE41_RS05835: ABIE41_RS05835 - hypothetical protein, at 1,200,285 to 1,200,434
_RS05835
ABIE41_RS05840: ABIE41_RS05840 - glucan biosynthesis protein D, at 1,200,522 to 1,202,102
_RS05840
ABIE41_RS05845: ABIE41_RS05845 - TAXI family TRAP transporter solute-binding subunit, at 1,202,381 to 1,203,349
_RS05845
Position (kb) 1200
1201
1202
1203 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1199.662 kb on + strand, within ABIE41_RS05830 at 1199.662 kb on + strand, within ABIE41_RS05830 at 1199.663 kb on - strand, within ABIE41_RS05830 at 1199.663 kb on - strand, within ABIE41_RS05830 at 1200.011 kb on + strand, within ABIE41_RS05830 at 1200.011 kb on + strand, within ABIE41_RS05830 at 1200.011 kb on + strand, within ABIE41_RS05830 at 1200.011 kb on + strand, within ABIE41_RS05830 at 1200.011 kb on + strand, within ABIE41_RS05830 at 1200.011 kb on + strand, within ABIE41_RS05830 at 1200.012 kb on - strand, within ABIE41_RS05830 at 1200.012 kb on - strand, within ABIE41_RS05830 at 1200.012 kb on - strand, within ABIE41_RS05830 at 1200.059 kb on + strand, within ABIE41_RS05830 at 1200.060 kb on - strand, within ABIE41_RS05830 at 1200.060 kb on - strand, within ABIE41_RS05830 at 1200.231 kb on - strand at 1200.231 kb on - strand at 1200.231 kb on - strand at 1200.231 kb on - strand at 1200.235 kb on + strand at 1200.235 kb on + strand at 1200.236 kb on - strand at 1200.236 kb on - strand at 1200.236 kb on - strand at 1200.536 kb on - strand at 1200.536 kb on - strand at 1200.536 kb on - strand at 1200.536 kb on - strand at 1200.541 kb on + strand at 1200.541 kb on + strand at 1200.683 kb on - strand, within ABIE41_RS05840 at 1200.683 kb on - strand, within ABIE41_RS05840 at 1200.683 kb on - strand, within ABIE41_RS05840 at 1200.688 kb on + strand, within ABIE41_RS05840 at 1200.689 kb on - strand, within ABIE41_RS05840 at 1200.892 kb on + strand, within ABIE41_RS05840 at 1200.893 kb on - strand, within ABIE41_RS05840 at 1200.893 kb on - strand, within ABIE41_RS05840 at 1200.904 kb on + strand, within ABIE41_RS05840 at 1200.905 kb on - strand, within ABIE41_RS05840 at 1200.905 kb on - strand, within ABIE41_RS05840 at 1201.054 kb on + strand, within ABIE41_RS05840 at 1201.054 kb on + strand, within ABIE41_RS05840 at 1201.072 kb on + strand, within ABIE41_RS05840 at 1201.072 kb on + strand, within ABIE41_RS05840 at 1201.072 kb on + strand, within ABIE41_RS05840 at 1201.073 kb on - strand, within ABIE41_RS05840 at 1201.276 kb on + strand, within ABIE41_RS05840 at 1201.276 kb on + strand, within ABIE41_RS05840 at 1201.276 kb on + strand, within ABIE41_RS05840 at 1201.276 kb on + strand, within ABIE41_RS05840 at 1201.276 kb on + strand, within ABIE41_RS05840 at 1201.277 kb on - strand, within ABIE41_RS05840 at 1201.277 kb on - strand, within ABIE41_RS05840 at 1201.277 kb on - strand, within ABIE41_RS05840 at 1201.277 kb on - strand, within ABIE41_RS05840 at 1201.277 kb on - strand, within ABIE41_RS05840 at 1201.277 kb on - strand, within ABIE41_RS05840 at 1201.420 kb on + strand, within ABIE41_RS05840 at 1201.421 kb on - strand, within ABIE41_RS05840 at 1201.421 kb on - strand, within ABIE41_RS05840 at 1201.464 kb on + strand, within ABIE41_RS05840 at 1201.464 kb on + strand, within ABIE41_RS05840 at 1201.464 kb on + strand, within ABIE41_RS05840 at 1201.464 kb on + strand, within ABIE41_RS05840 at 1201.464 kb on + strand, within ABIE41_RS05840 at 1201.464 kb on + strand, within ABIE41_RS05840 at 1201.464 kb on + strand, within ABIE41_RS05840 at 1201.464 kb on + strand, within ABIE41_RS05840 at 1201.464 kb on + strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.465 kb on - strand, within ABIE41_RS05840 at 1201.466 kb on + strand, within ABIE41_RS05840 at 1201.466 kb on + strand, within ABIE41_RS05840 at 1201.466 kb on + strand, within ABIE41_RS05840 at 1201.466 kb on + strand, within ABIE41_RS05840 at 1201.466 kb on + strand, within ABIE41_RS05840 at 1201.466 kb on + strand, within ABIE41_RS05840 at 1201.466 kb on + strand, within ABIE41_RS05840 at 1201.466 kb on + strand, within ABIE41_RS05840 at 1201.467 kb on - strand, within ABIE41_RS05840 at 1201.467 kb on - strand, within ABIE41_RS05840 at 1201.467 kb on - strand, within ABIE41_RS05840 at 1201.469 kb on - strand, within ABIE41_RS05840 at 1201.549 kb on + strand, within ABIE41_RS05840 at 1201.550 kb on - strand, within ABIE41_RS05840 at 1201.573 kb on + strand, within ABIE41_RS05840 at 1201.574 kb on - strand, within ABIE41_RS05840 at 1201.574 kb on - strand, within ABIE41_RS05840 at 1201.732 kb on + strand, within ABIE41_RS05840 at 1201.732 kb on + strand, within ABIE41_RS05840 at 1201.732 kb on + strand, within ABIE41_RS05840 at 1201.733 kb on - strand, within ABIE41_RS05840 at 1201.745 kb on - strand, within ABIE41_RS05840 at 1201.948 kb on + strand at 1202.329 kb on + strand at 1202.329 kb on + strand at 1202.330 kb on - strand at 1202.377 kb on + strand at 1202.378 kb on - strand at 1202.378 kb on - strand at 1202.380 kb on + strand at 1202.380 kb on + strand at 1202.380 kb on + strand at 1202.380 kb on + strand at 1202.380 kb on + strand at 1202.381 kb on - strand at 1202.381 kb on - strand at 1202.381 kb on - strand at 1202.381 kb on - strand at 1202.381 kb on - strand at 1202.507 kb on + strand, within ABIE41_RS05845 at 1202.507 kb on + strand, within ABIE41_RS05845 at 1202.507 kb on + strand, within ABIE41_RS05845 at 1202.508 kb on - strand, within ABIE41_RS05845 at 1202.508 kb on - strand, within ABIE41_RS05845 at 1202.510 kb on + strand, within ABIE41_RS05845 at 1202.513 kb on - strand, within ABIE41_RS05845 at 1202.540 kb on + strand, within ABIE41_RS05845 at 1202.540 kb on + strand, within ABIE41_RS05845 at 1202.540 kb on + strand, within ABIE41_RS05845 at 1202.540 kb on + strand, within ABIE41_RS05845 at 1202.540 kb on + strand, within ABIE41_RS05845 at 1202.540 kb on + strand, within ABIE41_RS05845 at 1202.540 kb on + strand, within ABIE41_RS05845 at 1202.541 kb on - strand, within ABIE41_RS05845 at 1202.541 kb on - strand, within ABIE41_RS05845 at 1202.541 kb on - strand, within ABIE41_RS05845 at 1202.541 kb on - strand, within ABIE41_RS05845 at 1202.735 kb on + strand, within ABIE41_RS05845 at 1202.735 kb on + strand, within ABIE41_RS05845 at 1202.744 kb on + strand, within ABIE41_RS05845 at 1202.744 kb on + strand, within ABIE41_RS05845 at 1202.744 kb on + strand, within ABIE41_RS05845 at 1202.745 kb on - strand, within ABIE41_RS05845 at 1202.745 kb on - strand, within ABIE41_RS05845 at 1202.745 kb on - strand, within ABIE41_RS05845 at 1202.882 kb on + strand, within ABIE41_RS05845 at 1202.882 kb on + strand, within ABIE41_RS05845 at 1202.882 kb on + strand, within ABIE41_RS05845 at 1202.882 kb on + strand, within ABIE41_RS05845 at 1202.883 kb on - strand, within ABIE41_RS05845 at 1202.883 kb on - strand, within ABIE41_RS05845 at 1202.883 kb on - strand, within ABIE41_RS05845 at 1202.883 kb on - strand, within ABIE41_RS05845 at 1202.883 kb on - strand, within ABIE41_RS05845 at 1202.883 kb on - strand, within ABIE41_RS05845 at 1202.883 kb on - strand, within ABIE41_RS05845 at 1202.906 kb on + strand, within ABIE41_RS05845 at 1202.906 kb on + strand, within ABIE41_RS05845 at 1202.907 kb on - strand, within ABIE41_RS05845 at 1202.907 kb on - strand, within ABIE41_RS05845 at 1203.101 kb on + strand, within ABIE41_RS05845 at 1203.101 kb on + strand, within ABIE41_RS05845 at 1203.101 kb on + strand, within ABIE41_RS05845 at 1203.101 kb on + strand, within ABIE41_RS05845 at 1203.101 kb on + strand, within ABIE41_RS05845 at 1203.101 kb on + strand, within ABIE41_RS05845 at 1203.101 kb on + strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845 at 1203.102 kb on - strand, within ABIE41_RS05845
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM remove 1,199,662 + ABIE41_RS05830 0.18 +1.4 1,199,662 + ABIE41_RS05830 0.18 +0.4 1,199,663 - ABIE41_RS05830 0.18 -0.1 1,199,663 - ABIE41_RS05830 0.18 +0.5 1,200,011 + ABIE41_RS05830 0.65 -0.3 1,200,011 + ABIE41_RS05830 0.65 -0.1 1,200,011 + ABIE41_RS05830 0.65 +1.1 1,200,011 + ABIE41_RS05830 0.65 +0.0 1,200,011 + ABIE41_RS05830 0.65 -0.4 1,200,011 + ABIE41_RS05830 0.65 -0.1 1,200,012 - ABIE41_RS05830 0.65 +0.1 1,200,012 - ABIE41_RS05830 0.65 +0.7 1,200,012 - ABIE41_RS05830 0.65 +0.1 1,200,059 + ABIE41_RS05830 0.72 -0.1 1,200,060 - ABIE41_RS05830 0.72 -0.1 1,200,060 - ABIE41_RS05830 0.72 +0.1 1,200,231 - +0.0 1,200,231 - +0.2 1,200,231 - -0.4 1,200,231 - +0.6 1,200,235 + -1.0 1,200,235 + +0.0 1,200,236 - -0.3 1,200,236 - -2.2 1,200,236 - -1.4 1,200,536 - +0.3 1,200,536 - +2.2 1,200,536 - -2.6 1,200,536 - -0.6 1,200,541 + -2.1 1,200,541 + -1.1 1,200,683 - ABIE41_RS05840 0.10 -0.7 1,200,683 - ABIE41_RS05840 0.10 -0.4 1,200,683 - ABIE41_RS05840 0.10 -0.4 1,200,688 + ABIE41_RS05840 0.10 -1.2 1,200,689 - ABIE41_RS05840 0.11 -0.4 1,200,892 + ABIE41_RS05840 0.23 -1.2 1,200,893 - ABIE41_RS05840 0.23 -1.0 1,200,893 - ABIE41_RS05840 0.23 -1.0 1,200,904 + ABIE41_RS05840 0.24 +0.0 1,200,905 - ABIE41_RS05840 0.24 -2.1 1,200,905 - ABIE41_RS05840 0.24 +0.0 1,201,054 + ABIE41_RS05840 0.34 -2.5 1,201,054 + ABIE41_RS05840 0.34 -2.1 1,201,072 + ABIE41_RS05840 0.35 -0.4 1,201,072 + ABIE41_RS05840 0.35 -0.1 1,201,072 + ABIE41_RS05840 0.35 -2.2 1,201,073 - ABIE41_RS05840 0.35 -2.4 1,201,276 + ABIE41_RS05840 0.48 -2.2 1,201,276 + ABIE41_RS05840 0.48 -2.4 1,201,276 + ABIE41_RS05840 0.48 -0.4 1,201,276 + ABIE41_RS05840 0.48 -2.4 1,201,276 + ABIE41_RS05840 0.48 -2.5 1,201,277 - ABIE41_RS05840 0.48 -1.8 1,201,277 - ABIE41_RS05840 0.48 +0.6 1,201,277 - ABIE41_RS05840 0.48 -0.7 1,201,277 - ABIE41_RS05840 0.48 -1.6 1,201,277 - ABIE41_RS05840 0.48 -0.8 1,201,277 - ABIE41_RS05840 0.48 +0.3 1,201,420 + ABIE41_RS05840 0.57 -2.0 1,201,421 - ABIE41_RS05840 0.57 -0.2 1,201,421 - ABIE41_RS05840 0.57 -2.6 1,201,464 + ABIE41_RS05840 0.60 +0.3 1,201,464 + ABIE41_RS05840 0.60 -2.9 1,201,464 + ABIE41_RS05840 0.60 +0.3 1,201,464 + ABIE41_RS05840 0.60 -1.2 1,201,464 + ABIE41_RS05840 0.60 -2.1 1,201,464 + ABIE41_RS05840 0.60 -0.9 1,201,464 + ABIE41_RS05840 0.60 -0.4 1,201,464 + ABIE41_RS05840 0.60 +1.1 1,201,464 + ABIE41_RS05840 0.60 -2.0 1,201,465 - ABIE41_RS05840 0.60 -2.2 1,201,465 - ABIE41_RS05840 0.60 -1.7 1,201,465 - ABIE41_RS05840 0.60 -1.6 1,201,465 - ABIE41_RS05840 0.60 -1.0 1,201,465 - ABIE41_RS05840 0.60 +0.0 1,201,465 - ABIE41_RS05840 0.60 -1.0 1,201,465 - ABIE41_RS05840 0.60 -0.8 1,201,465 - ABIE41_RS05840 0.60 -0.4 1,201,465 - ABIE41_RS05840 0.60 -1.2 1,201,465 - ABIE41_RS05840 0.60 -2.2 1,201,465 - ABIE41_RS05840 0.60 -0.6 1,201,465 - ABIE41_RS05840 0.60 -0.6 1,201,466 + ABIE41_RS05840 0.60 +0.6 1,201,466 + ABIE41_RS05840 0.60 -2.3 1,201,466 + ABIE41_RS05840 0.60 -2.0 1,201,466 + ABIE41_RS05840 0.60 -2.7 1,201,466 + ABIE41_RS05840 0.60 -1.0 1,201,466 + ABIE41_RS05840 0.60 -1.9 1,201,466 + ABIE41_RS05840 0.60 -2.3 1,201,466 + ABIE41_RS05840 0.60 -0.3 1,201,467 - ABIE41_RS05840 0.60 -1.2 1,201,467 - ABIE41_RS05840 0.60 -1.4 1,201,467 - ABIE41_RS05840 0.60 -1.7 1,201,469 - ABIE41_RS05840 0.60 -0.4 1,201,549 + ABIE41_RS05840 0.65 -1.4 1,201,550 - ABIE41_RS05840 0.65 -0.4 1,201,573 + ABIE41_RS05840 0.66 +0.2 1,201,574 - ABIE41_RS05840 0.67 -0.1 1,201,574 - ABIE41_RS05840 0.67 +1.0 1,201,732 + ABIE41_RS05840 0.77 -1.0 1,201,732 + ABIE41_RS05840 0.77 -1.0 1,201,732 + ABIE41_RS05840 0.77 -0.7 1,201,733 - ABIE41_RS05840 0.77 -0.5 1,201,745 - ABIE41_RS05840 0.77 -1.5 1,201,948 + -1.0 1,202,329 + +0.6 1,202,329 + +0.2 1,202,330 - -0.1 1,202,377 + +0.1 1,202,378 - +0.1 1,202,378 - -0.4 1,202,380 + +0.8 1,202,380 + -0.9 1,202,380 + -0.2 1,202,380 + +1.4 1,202,380 + -0.3 1,202,381 - -0.3 1,202,381 - +0.0 1,202,381 - -0.9 1,202,381 - +0.6 1,202,381 - -0.2 1,202,507 + ABIE41_RS05845 0.13 +0.0 1,202,507 + ABIE41_RS05845 0.13 +0.4 1,202,507 + ABIE41_RS05845 0.13 -0.2 1,202,508 - ABIE41_RS05845 0.13 +1.1 1,202,508 - ABIE41_RS05845 0.13 +0.3 1,202,510 + ABIE41_RS05845 0.13 +0.3 1,202,513 - ABIE41_RS05845 0.14 -0.0 1,202,540 + ABIE41_RS05845 0.16 +0.7 1,202,540 + ABIE41_RS05845 0.16 +0.0 1,202,540 + ABIE41_RS05845 0.16 +0.0 1,202,540 + ABIE41_RS05845 0.16 -0.8 1,202,540 + ABIE41_RS05845 0.16 -0.3 1,202,540 + ABIE41_RS05845 0.16 -0.2 1,202,540 + ABIE41_RS05845 0.16 -0.1 1,202,541 - ABIE41_RS05845 0.17 +0.4 1,202,541 - ABIE41_RS05845 0.17 -0.7 1,202,541 - ABIE41_RS05845 0.17 -0.0 1,202,541 - ABIE41_RS05845 0.17 -0.5 1,202,735 + ABIE41_RS05845 0.37 +1.3 1,202,735 + ABIE41_RS05845 0.37 -0.1 1,202,744 + ABIE41_RS05845 0.37 +0.2 1,202,744 + ABIE41_RS05845 0.37 +1.4 1,202,744 + ABIE41_RS05845 0.37 +1.4 1,202,745 - ABIE41_RS05845 0.38 -0.2 1,202,745 - ABIE41_RS05845 0.38 -0.0 1,202,745 - ABIE41_RS05845 0.38 +1.0 1,202,882 + ABIE41_RS05845 0.52 +0.0 1,202,882 + ABIE41_RS05845 0.52 +0.1 1,202,882 + ABIE41_RS05845 0.52 +0.5 1,202,882 + ABIE41_RS05845 0.52 +0.6 1,202,883 - ABIE41_RS05845 0.52 +0.1 1,202,883 - ABIE41_RS05845 0.52 -0.5 1,202,883 - ABIE41_RS05845 0.52 -0.5 1,202,883 - ABIE41_RS05845 0.52 +1.0 1,202,883 - ABIE41_RS05845 0.52 +0.2 1,202,883 - ABIE41_RS05845 0.52 +0.0 1,202,883 - ABIE41_RS05845 0.52 -0.4 1,202,906 + ABIE41_RS05845 0.54 +1.6 1,202,906 + ABIE41_RS05845 0.54 +0.7 1,202,907 - ABIE41_RS05845 0.54 -0.2 1,202,907 - ABIE41_RS05845 0.54 +0.1 1,203,101 + ABIE41_RS05845 0.74 -0.3 1,203,101 + ABIE41_RS05845 0.74 -0.5 1,203,101 + ABIE41_RS05845 0.74 +0.3 1,203,101 + ABIE41_RS05845 0.74 +0.9 1,203,101 + ABIE41_RS05845 0.74 -1.4 1,203,101 + ABIE41_RS05845 0.74 -0.1 1,203,101 + ABIE41_RS05845 0.74 -0.0 1,203,102 - ABIE41_RS05845 0.74 -0.6 1,203,102 - ABIE41_RS05845 0.74 +0.1 1,203,102 - ABIE41_RS05845 0.74 -0.5 1,203,102 - ABIE41_RS05845 0.74 -0.4 1,203,102 - ABIE41_RS05845 0.74 -0.5 1,203,102 - ABIE41_RS05845 0.74 +0.3 1,203,102 - ABIE41_RS05845 0.74 +1.0 1,203,102 - ABIE41_RS05845 0.74 +0.2 1,203,102 - ABIE41_RS05845 0.74 +0.3 1,203,102 - ABIE41_RS05845 0.74 -0.7 1,203,102 - ABIE41_RS05845 0.74 +0.6 1,203,102 - ABIE41_RS05845 0.74 +0.2 1,203,102 - ABIE41_RS05845 0.74 -0.8
Or see this region's nucleotide sequence