Strain Fitness in Escherichia coli BL21 around ECD_04212

Experiment: Laval1374

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_04211 and ECD_04212 overlap by 1 nucleotidesECD_04212 and ECD_04213 are separated by 161 nucleotides ECD_04211: ECD_04211 - 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC, at 4,483,222 to 4,484,268 _04211 ECD_04212: ECD_04212 - 5-methylcytosine-specific restriction enzyme McrBC, subunit McrB, at 4,484,268 to 4,485,647 _04212 ECD_04213: ECD_04213 - toxic peptide regulated by antisense sRNA symR, at 4,485,809 to 4,486,150 _04213 Position (kb) 4484 4485 4486Strain fitness (log2 ratio) -1 0 1at 4483.285 kb on + strandat 4483.351 kb on + strand, within ECD_04211at 4483.362 kb on - strand, within ECD_04211at 4484.251 kb on + strandat 4484.299 kb on - strandat 4484.669 kb on - strand, within ECD_04212at 4484.942 kb on - strand, within ECD_04212at 4485.734 kb on + strandat 4485.761 kb on + strandat 4485.952 kb on + strand, within ECD_04213at 4485.953 kb on - strand, within ECD_04213at 4486.051 kb on - strand, within ECD_04213at 4486.071 kb on + strand, within ECD_04213at 4486.154 kb on + strandat 4486.154 kb on + strandat 4486.564 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Laval1374
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4,483,285 + -0.1
4,483,351 + ECD_04211 0.12 -0.3
4,483,362 - ECD_04211 0.13 -0.3
4,484,251 + -0.6
4,484,299 - -0.7
4,484,669 - ECD_04212 0.29 +0.8
4,484,942 - ECD_04212 0.49 +1.5
4,485,734 + +0.4
4,485,761 + +0.1
4,485,952 + ECD_04213 0.42 +0.2
4,485,953 - ECD_04213 0.42 -0.3
4,486,051 - ECD_04213 0.71 +1.2
4,486,071 + ECD_04213 0.77 -0.2
4,486,154 + +0.5
4,486,154 + +0.7
4,486,564 + -0.3

Or see this region's nucleotide sequence