Strain Fitness in Escherichia coli BL21 around ECD_04177

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_04176 and ECD_04177 are separated by 64 nucleotidesECD_04177 and ECD_04178 are separated by 19 nucleotides ECD_04176: ECD_04176 - 9-O-acetyl N-acetylneuraminic acid esterase, at 4,445,117 to 4,446,097 _04176 ECD_04177: ECD_04177 - N-acetylneuraminic acid mutarotase, at 4,446,162 to 4,447,268 _04177 ECD_04178: ECD_04178 - N-acetylnuraminic acid outer membrane channel protein, at 4,447,288 to 4,448,004 _04178 Position (kb) 4446 4447 4448Strain fitness (log2 ratio) -1 0 1 2 3at 4445.250 kb on + strand, within ECD_04176at 4445.264 kb on + strand, within ECD_04176at 4445.265 kb on - strand, within ECD_04176at 4445.382 kb on + strand, within ECD_04176at 4445.452 kb on + strand, within ECD_04176at 4445.453 kb on - strand, within ECD_04176at 4445.456 kb on - strand, within ECD_04176at 4445.478 kb on - strand, within ECD_04176at 4445.503 kb on + strand, within ECD_04176at 4445.630 kb on + strand, within ECD_04176at 4445.700 kb on + strand, within ECD_04176at 4445.700 kb on + strand, within ECD_04176at 4445.701 kb on - strand, within ECD_04176at 4445.804 kb on - strand, within ECD_04176at 4445.830 kb on - strand, within ECD_04176at 4445.911 kb on + strand, within ECD_04176at 4445.911 kb on + strand, within ECD_04176at 4445.911 kb on + strand, within ECD_04176at 4445.912 kb on - strand, within ECD_04176at 4445.966 kb on + strand, within ECD_04176at 4445.974 kb on + strand, within ECD_04176at 4446.041 kb on - strandat 4446.070 kb on - strandat 4446.087 kb on - strandat 4446.260 kb on + strandat 4446.261 kb on - strandat 4446.280 kb on - strand, within ECD_04177at 4446.386 kb on - strand, within ECD_04177at 4446.516 kb on - strand, within ECD_04177at 4446.762 kb on - strand, within ECD_04177at 4446.847 kb on + strand, within ECD_04177at 4446.848 kb on - strand, within ECD_04177at 4446.940 kb on + strand, within ECD_04177at 4446.941 kb on - strand, within ECD_04177at 4446.949 kb on - strand, within ECD_04177at 4447.022 kb on + strand, within ECD_04177at 4447.377 kb on - strand, within ECD_04178at 4447.393 kb on - strand, within ECD_04178at 4447.485 kb on - strand, within ECD_04178at 4447.486 kb on + strand, within ECD_04178at 4447.521 kb on + strand, within ECD_04178at 4447.530 kb on - strand, within ECD_04178

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,445,250 + ECD_04176 0.14 -0.4
4,445,264 + ECD_04176 0.15 -0.1
4,445,265 - ECD_04176 0.15 -0.5
4,445,382 + ECD_04176 0.27 +0.6
4,445,452 + ECD_04176 0.34 +0.6
4,445,453 - ECD_04176 0.34 -0.3
4,445,456 - ECD_04176 0.35 +0.1
4,445,478 - ECD_04176 0.37 +0.4
4,445,503 + ECD_04176 0.39 +0.3
4,445,630 + ECD_04176 0.52 +0.2
4,445,700 + ECD_04176 0.59 -0.6
4,445,700 + ECD_04176 0.59 +0.3
4,445,701 - ECD_04176 0.60 +0.4
4,445,804 - ECD_04176 0.70 +0.9
4,445,830 - ECD_04176 0.73 +0.4
4,445,911 + ECD_04176 0.81 -0.2
4,445,911 + ECD_04176 0.81 -0.4
4,445,911 + ECD_04176 0.81 -0.2
4,445,912 - ECD_04176 0.81 +1.9
4,445,966 + ECD_04176 0.87 +0.6
4,445,974 + ECD_04176 0.87 +1.2
4,446,041 - -0.7
4,446,070 - -0.1
4,446,087 - -0.4
4,446,260 + +1.4
4,446,261 - +0.8
4,446,280 - ECD_04177 0.11 +0.5
4,446,386 - ECD_04177 0.20 +1.4
4,446,516 - ECD_04177 0.32 +1.6
4,446,762 - ECD_04177 0.54 -1.0
4,446,847 + ECD_04177 0.62 +3.0
4,446,848 - ECD_04177 0.62 -0.6
4,446,940 + ECD_04177 0.70 +0.7
4,446,941 - ECD_04177 0.70 -0.7
4,446,949 - ECD_04177 0.71 +0.7
4,447,022 + ECD_04177 0.78 +1.4
4,447,377 - ECD_04178 0.12 -0.4
4,447,393 - ECD_04178 0.15 +0.4
4,447,485 - ECD_04178 0.27 +0.2
4,447,486 + ECD_04178 0.28 +0.4
4,447,521 + ECD_04178 0.32 +1.2
4,447,530 - ECD_04178 0.34 +0.0

Or see this region's nucleotide sequence