Strain Fitness in Escherichia coli BL21 around ECD_00698

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00696 and ECD_00697 overlap by 4 nucleotidesECD_00697 and ECD_00698 are separated by 3 nucleotidesECD_00698 and ECD_00699 are separated by 64 nucleotides ECD_00696: ECD_00696 - acyl-CoA thioester hydrolase, at 733,413 to 733,817 _00696 ECD_00697: ECD_00697 - membrane spanning protein in TolA-TolQ-TolR complex, at 733,814 to 734,506 _00697 ECD_00698: ECD_00698 - membrane spanning protein in TolA-TolQ-TolR complex, at 734,510 to 734,938 _00698 ECD_00699: ECD_00699 - membrane anchored protein in TolA-TolQ-TolR complex, at 735,003 to 736,268 _00699 Position (kb) 734 735Strain fitness (log2 ratio) -1 0 1 2at 733.588 kb on + strand, within ECD_00696at 733.592 kb on + strand, within ECD_00696at 733.608 kb on + strand, within ECD_00696at 733.657 kb on + strand, within ECD_00696at 733.697 kb on + strand, within ECD_00696at 733.715 kb on + strand, within ECD_00696at 734.936 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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733,588 + ECD_00696 0.43 -0.0
733,592 + ECD_00696 0.44 +1.4
733,608 + ECD_00696 0.48 -0.0
733,657 + ECD_00696 0.60 +1.8
733,697 + ECD_00696 0.70 -0.0
733,715 + ECD_00696 0.75 -0.3
734,936 + +0.4

Or see this region's nucleotide sequence