Strain Fitness in Escherichia coli BL21 around ECD_00454

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00453 and ECD_00454 are separated by 68 nucleotidesECD_00454 and ECD_00455 are separated by 229 nucleotidesECD_00455 and ECD_00456 are separated by 77 nucleotides ECD_00453: ECD_00453 - tRNA 2-selenouridine synthase, selenophosphate-dependent, at 499,277 to 500,371 _00453 ECD_00454: ECD_00454 - allD operon transcriptional activator, at 500,440 to 501,366 _00454 ECD_00455: ECD_00455 - ureidoglycolate lyase, releasing urea, at 501,596 to 502,078 _00455 ECD_00456: ECD_00456 - glyoxylate-inducible transcriptional repressor of all and gcl operons, at 502,156 to 502,971 _00456 Position (kb) 500 501 502Strain fitness (log2 ratio) -2 -1 0 1at 499.539 kb on + strand, within ECD_00453at 499.566 kb on + strand, within ECD_00453at 499.647 kb on - strand, within ECD_00453at 499.941 kb on - strand, within ECD_00453at 500.056 kb on + strand, within ECD_00453at 500.057 kb on - strand, within ECD_00453at 500.092 kb on + strand, within ECD_00453at 500.093 kb on - strand, within ECD_00453at 500.206 kb on + strand, within ECD_00453at 500.209 kb on - strand, within ECD_00453at 500.312 kb on - strandat 500.320 kb on + strandat 500.371 kb on + strandat 500.372 kb on - strandat 500.372 kb on - strandat 500.442 kb on - strandat 500.468 kb on - strandat 500.514 kb on + strandat 500.514 kb on + strandat 500.706 kb on + strand, within ECD_00454at 500.895 kb on + strand, within ECD_00454at 500.896 kb on - strand, within ECD_00454at 500.949 kb on - strand, within ECD_00454at 500.949 kb on - strand, within ECD_00454at 500.975 kb on + strand, within ECD_00454at 501.173 kb on - strand, within ECD_00454at 501.290 kb on - strandat 501.576 kb on + strandat 501.582 kb on - strandat 501.582 kb on - strandat 501.609 kb on + strandat 501.634 kb on + strandat 501.947 kb on + strand, within ECD_00455at 501.955 kb on + strand, within ECD_00455at 502.049 kb on - strandat 502.099 kb on - strandat 502.108 kb on + strandat 502.109 kb on - strandat 502.123 kb on + strandat 502.151 kb on + strandat 502.151 kb on + strandat 502.152 kb on - strandat 502.155 kb on + strandat 502.252 kb on + strand, within ECD_00456

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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499,539 + ECD_00453 0.24 -0.5
499,566 + ECD_00453 0.26 +0.2
499,647 - ECD_00453 0.34 +0.4
499,941 - ECD_00453 0.61 -0.5
500,056 + ECD_00453 0.71 +0.8
500,057 - ECD_00453 0.71 -0.2
500,092 + ECD_00453 0.74 +1.4
500,093 - ECD_00453 0.75 -0.1
500,206 + ECD_00453 0.85 +1.6
500,209 - ECD_00453 0.85 +0.9
500,312 - +0.6
500,320 + +0.8
500,371 + +1.4
500,372 - +0.1
500,372 - -1.6
500,442 - +1.2
500,468 - +0.7
500,514 + +0.2
500,514 + -0.1
500,706 + ECD_00454 0.29 +0.7
500,895 + ECD_00454 0.49 +1.3
500,896 - ECD_00454 0.49 +0.2
500,949 - ECD_00454 0.55 -0.5
500,949 - ECD_00454 0.55 -0.4
500,975 + ECD_00454 0.58 -0.5
501,173 - ECD_00454 0.79 -0.4
501,290 - -0.7
501,576 + -0.4
501,582 - -0.1
501,582 - -2.1
501,609 + -0.8
501,634 + -0.1
501,947 + ECD_00455 0.73 +0.2
501,955 + ECD_00455 0.74 +0.7
502,049 - +0.2
502,099 - +0.5
502,108 + +0.5
502,109 - -0.8
502,123 + -1.0
502,151 + +0.2
502,151 + +0.1
502,152 - -0.1
502,155 + +0.2
502,252 + ECD_00456 0.12 -2.5

Or see this region's nucleotide sequence